{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:18:08Z","timestamp":1790295488274,"version":"4.1.0"},"reference-count":89,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T00:00:00Z","timestamp":1774915200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T00:00:00Z","timestamp":1774915200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Coagulase\u2010negative staphylococci (CoNS) are ubiquitous skin commensals but can cause opportunistic infections and may serve as reservoirs for mobile genetic elements such as the staphylococcal cassette chromosome\n                    <jats:italic>mec<\/jats:italic>\n                    (SCC\n                    <jats:italic>mec<\/jats:italic>\n                    ) associated with healthcare\u2010adapted lineages. In this longitudinal, single\u2010participant case study, we characterized CoNS isolates obtained over time from a healthy 32\u2010year\u2010old male who experienced two infection episodes 6 months apart (left ear, followed by the left eye), together with post\u2010recovery sampling from additional body sites. Six CoNS isolates were recovered, tested for antimicrobial susceptibility and subjected to whole\u2010genome sequencing. Genome\u2010based identification showed that the ear and arm isolates were\n                    <jats:italic>Staphylococcus capitis<\/jats:italic>\n                    (subsp.\n                    <jats:italic>urealyticus<\/jats:italic>\n                    and subsp.\n                    <jats:italic>capitis<\/jats:italic>\n                    ), whereas three isolates from the symptomatic left eye were\n                    <jats:italic>Staphylococcus epidermidis<\/jats:italic>\n                    . The three\n                    <jats:italic>S. capitis<\/jats:italic>\n                    isolates belonged to distinct sequence types (ST1, ST2, and ST10), while the three\n                    <jats:italic>S. epidermidis<\/jats:italic>\n                    isolates shared a novel sequence type, ST1284. A non\u2010typable, mosaic SCC\n                    <jats:italic>mec<\/jats:italic>\n                    type IV element homologous to SCC\n                    <jats:italic>mec<\/jats:italic>\n                    IVa\/IVn was identified in the multidrug\u2010resistant\n                    <jats:italic>S. capitis<\/jats:italic>\n                    subsp.\n                    <jats:italic>urealyticus<\/jats:italic>\n                    ST1 isolate E_e2, recovered from the infected ear. Core\u2010genome phylogeny and genomic signatures placed E_e2 within the proto\u2010NRCS\u2010A clade, indicating that a lineage closely related to hospital\u2010associated outbreak strains can be encountered in a community\u2010dwelling individual without known prior healthcare exposure. These findings provide a within\u2010host genomic snapshot of CoNS dynamics across contiguous anatomical sites, and while the study does not establish causality for infection or permit population\u2010level inference, it highlights the need for broader, systematic community surveillance of CoNS lineages and their mobile genetic elements.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.70277","type":"journal-article","created":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T04:48:35Z","timestamp":1775018915000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Of Commensals and Opportunists: Genomics of Coagulase\u2010Negative Staphylococci During Sequential Ear and Eye Infections in a Healthy Adult"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2239-2004","authenticated-orcid":false,"given":"Soo Sum","family":"Lean","sequence":"first","affiliation":[{"name":"Faculty of Medicine and Institute for Life Sciences, University of Southampton Southampton UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9644-6925","authenticated-orcid":false,"given":"Chew Chieng","family":"Yeo","sequence":"additional","affiliation":[{"name":"Centre for Research in Infectious Diseases &amp; Biotechnology (CeRIDB), Faculty of Medicine Universiti Sultan Zainal Abidin Kuala Terengganu Terengganu Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zain","family":"Illyaaseen","sequence":"additional","affiliation":[{"name":"Department of Pre\u2010Clinical Sciences, M. Kandiah Faculty of Medicine and Health Sciences Universiti Tunku Abdul Rahman Kajang Selangor Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sargit","family":"Kaur","sequence":"additional","affiliation":[{"name":"Department of Pre\u2010Clinical Sciences, M. Kandiah Faculty of Medicine and Health Sciences Universiti Tunku Abdul Rahman Kajang Selangor Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1011-762X","authenticated-orcid":false,"given":"Yun Fong","family":"Ngeow","sequence":"additional","affiliation":[{"name":"Department of Pre\u2010Clinical Sciences, M. Kandiah Faculty of Medicine and Health Sciences Universiti Tunku Abdul Rahman Kajang Selangor Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7009-1548","authenticated-orcid":false,"given":"Stuart C.","family":"Clarke","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and Institute for Life Sciences, University of Southampton Southampton UK"},{"name":"Centre for Research in Infectious Diseases &amp; Biotechnology (CeRIDB), Faculty of Medicine Universiti Sultan Zainal Abidin Kuala Terengganu Terengganu Malaysia"},{"name":"Department of Biological Sciences, Faculty of Science Universiti Tunku Abdul Rahman Kampar Perak Malaysia"},{"name":"Institute for Research, Development and Innovation International Medical University  Kuala Lumpur Malaysia"},{"name":"NIHR Southampton Biomedical Research Centre University Hospital Southampton Foundation NHS Trust Southampton UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7228-096X","authenticated-orcid":false,"given":"Hien Fuh","family":"Ng","sequence":"additional","affiliation":[{"name":"Department of Pre\u2010Clinical Sciences, M. 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