{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T01:05:57Z","timestamp":1791594357180,"version":"4.3.4"},"reference-count":50,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,4,19]],"date-time":"2026-04-19T00:00:00Z","timestamp":1776556800000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Extended\u2010spectrum \u03b2\u2010lactamase (ESBL)\u2013producing Gram\u2010negative bacteria pose a severe therapeutic challenge globally.\n                    <jats:italic>Streptomyces<\/jats:italic>\n                    remain one of the most prolific natural sources of antibacterial and antioxidant secondary metabolites, yet their activity against ESBL\u2010producing pathogens remains under\u2010explored. Soil\u2010derived\n                    <jats:italic>Streptomyces<\/jats:italic>\n                    isolates were screened for bioactivity, and the most potent strain (SM7) was identified by 16S rRNA sequencing. Secondary metabolites were extracted using ethyl\u2010acetate and evaluated for antibacterial activity against ESBL\u2010producing\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    ,\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    , and\n                    <jats:italic>Enterobacter cloacae<\/jats:italic>\n                    using agar diffusion, MIC, and MBC assays. Antioxidant activity was assessed using DPPH and ABTS assays, while GC\u2013MS and molecular docking were employed to identify and characterize bioactive compounds.\n                    <jats:italic>Streptomyces sp<\/jats:italic>\n                    . SM7 exhibited strong antibacterial activity, producing inhibition zones of 21.4\u2009\u00b1\u20090.6\u2009mm, 19.2\u2009\u00b1\u20090.4\u2009mm, and 17.6\u2009\u00b1\u20090.5\u2009mm against ESBL\u2010producing\n                    <jats:italic>E. coli<\/jats:italic>\n                    ,\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    , and\n                    <jats:italic>E. cloacae<\/jats:italic>\n                    , respectively. MIC values ranged from 62.5 to 250\u2009\u00b5g\/mL, with bactericidal MBC\/MIC ratios of 2. The extract showed potent antioxidant activity with DPPH and ABTS IC\u2085\u2080 values of 48.9\u2009\u00b5g\/mL and 61.4\u2009\u00b5g\/mL, respectively. GC\u2013MS identified 18 bioactive compounds, with 2,4\u2010di\u2010tert\u2010butylphenol (18.6%) as the major constituent, which exhibited a docking affinity of \u22127.1\u2009kcal\/mol against bacterial DHFR.\n                    <jats:italic>Streptomyces sp<\/jats:italic>\n                    . SM7 produces phenolic\u2010 and fatty\u2010acid\u2010rich metabolites with potent bactericidal and antioxidant activities against ESBL\u2010producing pathogens, highlighting its promise as a natural source of next\u2010generation antimicrobial agents. These findings support\n                    <jats:italic>Streptomyces<\/jats:italic>\n                    sp. SM7 as a promising lead for downstream purification, mechanism\u2010guided optimization, and future drug\u2010development efforts targeting difficult\u2010to\u2010treat ESBL\u2010producing Enterobacterales.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.70282","type":"journal-article","created":{"date-parts":[[2026,4,19]],"date-time":"2026-04-19T14:45:09Z","timestamp":1776609909000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Antioxidant and Antibacterial Activities of Secondary Metabolites Produced by\n                    <i>Streptomyces<\/i>\n                    Isolates Against Extended\u2010Spectrum \u03b2\u2010Lactamase\u2010Producing Bacteria"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5546-5778","authenticated-orcid":false,"given":"Abdirasak Sharif","family":"Ali","sequence":"first","affiliation":[{"name":"Faculty of Medicine and Health Sciences SIMAD 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