{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T10:48:11Z","timestamp":1782470891927,"version":"3.54.5"},"reference-count":65,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T00:00:00Z","timestamp":1779235200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T00:00:00Z","timestamp":1779235200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Food\u2010producing animals act as reservoirs of antimicrobial\u2010resistant bacteria, which may serve as sources of infection in humans. A selection of ESBL\/AmpC\u2010producing\n                    <jats:italic>E. coli<\/jats:italic>\n                    isolates recovered from healthy (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200937) and diseased animals (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200926) were subjected to long\u2010read whole\u2010genome sequencing to characterize their genomic diversity, phylogeny, resistome, mobilome, and the genetic environment of antimicrobial resistance genes (ARGs). Isolates showed high genetic diversity (36 STs, seven phylogroups, 42 serotypes, 24\n                    <jats:italic>fimH<\/jats:italic>\n                    types) and a diverse resistome, with CTX\u2010M enzymes as the most prevalent ESBL type and\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX\u2010M\u201014<\/jats:sub>\n                    and\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX\u2010M\u201065<\/jats:sub>\n                    predominating. Most ARGs were plasmid\u2010encoded (71.9%), with IncHI2 plasmids harboring the highest number of ARGs (8\u201320) and carrying complex multidrug resistance islands interspersed with integrons, transposons, and insertion sequences. All 15 IncHI2 plasmids were found exclusively in bovine isolates of multiple phylogroups (5) and STs (13), and most (13\/15) carried\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX\u2010M\u201065<\/jats:sub>\n                    . This finding suggests a dissemination of this ESBL gene through a shared plasmid rather than a single dominant clone. This study reveals an emerging circulation of\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX\u2010M\u201065<\/jats:sub>\n                    in bovine\n                    <jats:italic>E. coli<\/jats:italic>\n                    in Spain and identifies IncHI2 plasmids as major vehicles driving its spread. The presence of these plasmids in genetically diverse and potentially zoonotic linages reinforces their relevance from a One Health and food\u2010chain perspective.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.70314","type":"journal-article","created":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T06:30:48Z","timestamp":1779345048000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Emerging Dissemination of\n                    <i>bla<\/i>\n                    <sub>CTX\u2010M\u201065<\/sub>\n                    in Bovine\n                    <i>E. coli<\/i>\n                    in Spain Associated With IncHI2 Plasmids"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5136-956X","authenticated-orcid":false,"given":"Medelin","family":"Ocejo","sequence":"first","affiliation":[{"name":"Animal Health Department, NEIKER \u2013 Basque Institute for Agricultural Research and Development, Basque Research and Technology Alliance (BRTA) Bizkaia Science and Technology Park 812\u2009L Derio Bizkaia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8896-6245","authenticated-orcid":false,"given":"Beatriz","family":"Oporto","sequence":"additional","affiliation":[{"name":"Animal Health Department, NEIKER \u2013 Basque Institute for Agricultural Research and Development, Basque Research and Technology Alliance (BRTA) Bizkaia Science and Technology Park 812\u2009L Derio Bizkaia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7089-9670","authenticated-orcid":false,"given":"Ana","family":"Hurtado","sequence":"additional","affiliation":[{"name":"Animal Health Department, NEIKER \u2013 Basque Institute for Agricultural Research and Development, Basque Research and Technology Alliance (BRTA) Bizkaia Science and Technology Park 812\u2009L Derio Bizkaia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,20]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-59870-2"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1099\/MGEN.0.000192"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/JAC\/DKX146"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkaa345"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3201\/EID2209.160140"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2012.01.011"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/JAC\/DKS004"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2006.08.011"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/JACAMR\/DLAB092"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2020.e03206"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/JOURNAL.PONE.0011147"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/1541-4337.12438"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/ANTIBIOTICS14080753"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.PLASMID.2019.01.003"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.2903\/J.EFSA.2025.9237"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/S13568-019-0920-4"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/FMICB.2021.626774"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02068-17"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(02)00150-1"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/JAC\/DKQ101"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00152-08"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00995-17"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1371\/JOURNAL.PONE.0157693"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00036-08"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00008-15"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx1321"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/CID\/CIU785"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.3389\/FMICB.2021.653595"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1099\/MGEN.0.000475"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/J.1600-0463.2011.02856.X"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/9781555819804.ch25"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-10210-3"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/NAR\/GKAE1095"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.3390\/MICROORGANISMS10040700"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.7717\/PEERJ.5233"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv421"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1101\/GR.186072.114"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.4.1263-1270.2001"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00088-17"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/FMICB.2018.01549"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1371\/JOURNAL.PONE.0122369"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.1.1-11.2002"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1128\/9781555819804.ch13"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1099\/MGEN.0.000206"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00737-17"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1099\/MGEN.0.000685"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu153"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drup.2012.12.001"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1371\/JOURNAL.PONE.0163962"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1093\/BIOINFORMATICS\/BTU033"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1371\/JOURNAL.PONE.0077644"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00742-20"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.3389\/FMICB.2022.936843"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1186\/S13059-014-0524-X"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.3390\/ANTIBIOTICS4040567"},{"key":"e_1_2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