{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T10:48:29Z","timestamp":1782470909744,"version":"3.54.5"},"reference-count":44,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,5,24]],"date-time":"2026-05-24T00:00:00Z","timestamp":1779580800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,24]],"date-time":"2026-05-24T00:00:00Z","timestamp":1779580800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["101054719"],"award-info":[{"award-number":["101054719"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    While several bacteriocins have been identified from gut\u2010isolated cultures, there remains a need for the discovery of bacteriocins with varying inhibition spectra for strain applications such as microbiome editing, pathogen elimination and colonization resistance. With this in mind, here we describe a new antibacterial sactipeptide gnavucin D, produced by\n                    <jats:italic>Mediterraneibacter gnavus<\/jats:italic>\n                    HB038 isolated from a healthy 2\u2010year\u2010old child. Gnavucin D has activity against the pathogens\n                    <jats:italic>Clostridium perfringens<\/jats:italic>\n                    ,\n                    <jats:italic>Streptococcus agalactiae, Bacillus cereus<\/jats:italic>\n                    , and vancomycin\u2010resistant\n                    <jats:italic>Enterococcus<\/jats:italic>\n                    . The gene cluster includes five structural genes in tandem that encode for three different core peptides (29 amino acids each) with molecular masses of 2704.21 (D1), 2734.23 (D2\/D3), and 2732.21 (D4\/D5) Da. The nearest relative to these bacteriocins was found to be another sactipeptide thurincin H produced by\n                    <jats:italic>Bacillus thuringiensis<\/jats:italic>\n                    with which it shares 30% identity. Although the amino acids encoding the gnavucin and thurincin are similar with regard to putative functions, their homology to each other is low, varying from 30% to 55%. Interestingly, all these bacteriocins had short leader peptides of only 9 amino acids. Gnavucin D was found to be extremely stable to temperature, pH and proteolysis which is possibly a reflection of the sulfur to carbon post\u2010translational modifications. The observed molecular masses of the 3 different peptides correspond to four modifications, yielding a structurally restricted, most likely double\u2010hairpin conformation which is characteristic of such sactipeptides. Consequently, gnavucin D can be a promising candidate for selective antibacterial activity against human pathogens.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.70315","type":"journal-article","created":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T05:15:35Z","timestamp":1779686135000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Production of Multiple Variants of the Antimicrobial Sactipeptide Gnavucin D by the Human Gut Isolate\n                    <i>Mediterraneibacter gnavus<\/i>\n                    HB038"],"prefix":"10.1002","volume":"15","author":[{"given":"Mengfan","family":"Ding","sequence":"first","affiliation":[{"name":"APC Microbiome Ireland University College Cork Cork Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5419-9922","authenticated-orcid":false,"given":"Felipe Miceli","family":"de Farias","sequence":"additional","affiliation":[{"name":"APC Microbiome Ireland University College Cork Cork Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula M.","family":"O'Connor","sequence":"additional","affiliation":[{"name":"Teagasc Food Research Centre, Moorepark, Fermoy Cork Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohui","family":"Huang","sequence":"additional","affiliation":[{"name":"APC Microbiome Ireland University College Cork Cork Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fiona C.","family":"Ross","sequence":"additional","affiliation":[{"name":"APC Microbiome Ireland University College Cork Cork Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elaine C.","family":"Kennedy","sequence":"additional","affiliation":[{"name":"Department of Pediatrics and Child Health University College Cork Cork Ireland"},{"name":"INFANT Research Centre University College Cork Cork Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colin P.","family":"Hawkes","sequence":"additional","affiliation":[{"name":"Department of Pediatrics and Child Health University College Cork Cork Ireland"},{"name":"INFANT Research Centre University College Cork Cork Ireland"},{"name":"Perelman School of Medicine University of Pennsylvania Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colin","family":"Hill","sequence":"additional","affiliation":[{"name":"APC Microbiome Ireland University College Cork Cork Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Stanton","sequence":"additional","affiliation":[{"name":"APC Microbiome Ireland University College Cork Cork Ireland"},{"name":"Teagasc Food Research Centre, Moorepark, Fermoy Cork Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reynolds Paul","family":"Ross","sequence":"additional","affiliation":[{"name":"APC Microbiome Ireland University College Cork Cork Ireland"},{"name":"Teagasc Food Research Centre, Moorepark, Fermoy Cork Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,24]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12602-016-9242-x"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12602-010-9042-7"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA119.009416"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkad344"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aaw9969"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41557-022-01063-3"},{"issue":"1","key":"e_1_2_12_8_1","first-page":"A.3C.1","article-title":"Gram Staining","author":"Coico R.","year":"2006","journal-title":"Current Protocols in Microbiology"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fuad014"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab225"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btab007"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2017.01.004"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-021-00569-w"},{"key":"e_1_2_12_14_1","article-title":"Thuricin CD, an Antimicrobial for Specifically Targeting Clostridium Difficile","author":"Hill C.","year":"2014","journal-title":"In: Google Patents"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.001383"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi0359527"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2024-089206"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature15524"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.052258-0"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac0105181"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2009.01749.x"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9112276"},{"key":"e_1_2_12_23_1","unstructured":"Mathur H.(2014). Thuricin CD: A Potential Therapeutic Targeted Against Clostridium Difficile\u2010Associated Diarrhea (CDAD)."},{"key":"e_1_2_12_24_1","first-page":"8","article-title":"Insights Into the Mode of Action of the Sactibiotic Thuricin CD","volume":"696","author":"Mathur H.","year":"2017","journal-title":"Frontiers in Microbiology"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.17327"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/aem.00809-24"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms251910788"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201908490"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2024.1470988"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.2c00210"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12602-022-09952-2"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0913554107"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2004045117"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22063253"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1142\/13005"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-025-04372-6"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2023.107563"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkl495"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12602-010-9061-4"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-024-09656-8"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/9990635"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky383"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/1574-6968.12486"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp033"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-29325-1"}],"container-title":["MicrobiologyOpen"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mbo3.70315","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/mbo3.70315","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mbo3.70315","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T09:56:36Z","timestamp":1782467796000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mbo3.70315"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,24]]},"references-count":44,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1002\/mbo3.70315"],"URL":"https:\/\/doi.org\/10.1002\/mbo3.70315","archive":["Portico"],"relation":{},"ISSN":["2045-8827","2045-8827"],"issn-type":[{"value":"2045-8827","type":"print"},{"value":"2045-8827","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,24]]},"assertion":[{"value":"2026-02-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70315"}}