{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T10:47:40Z","timestamp":1782470860113,"version":"3.54.5"},"reference-count":19,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,6,21]],"date-time":"2026-06-21T00:00:00Z","timestamp":1782000000000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002742","name":"Banaras Hindu University","doi-asserted-by":"publisher","award":["III\/2022\u201023\/49235"],"award-info":[{"award-number":["III\/2022\u201023\/49235"]}],"id":[{"id":"10.13039\/501100002742","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Aspergillus nidulans<\/jats:italic>\n                    is a fungal pathogen that causes respiratory issues in individuals with compromised immune systems. It is identified through culture characteristics and microscopic features such as Cleistothecia and H\u00fclle Cells. However, similar traits are found in cryptic\n                    <jats:italic>Aspergillus<\/jats:italic>\n                    species, such as\n                    <jats:italic>stellatus, cristatus<\/jats:italic>\n                    , and\n                    <jats:italic>oryzae<\/jats:italic>\n                    . In a case series from a hospital in North India,\n                    <jats:italic>A. nidulans<\/jats:italic>\n                    strains isolated from patients with pulmonary aspergillosis were phenotypically identified and then underwent molecular characterization through sequencing of the amplified ITS1\u20105.8S rDNA\u2010ITS2 region and antifungal susceptibility testing (AST) according to the CLSI M38A3 guidelines. The molecular characteristics and antifungal profiles of the nine phenotypic\n                    <jats:italic>A. nidulans<\/jats:italic>\n                    were as follows:\n                    <jats:italic>A. stellatus<\/jats:italic>\n                    (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20095),\n                    <jats:italic>A. nidulans<\/jats:italic>\n                    (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20092),\n                    <jats:italic>A. cristatus<\/jats:italic>\n                    (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20091), and\n                    <jats:italic>A. oryzae<\/jats:italic>\n                    (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20091). Phylogenetic analysis revealed a close relationship between\n                    <jats:italic>A. stellatus, A. nidulans<\/jats:italic>\n                    , and\n                    <jats:italic>A. cristatus<\/jats:italic>\n                    , while\n                    <jats:italic>A. oryzae<\/jats:italic>\n                    showed significant divergence. Furthermore, the minimum inhibitory concentration (MIC) of antifungals was the lowest for caspofungin, followed by voriconazole. However, a higher amphotericin B MIC (2\u2009\u00b5g\/mL) was observed for\n                    <jats:italic>A. stellatus<\/jats:italic>\n                    . Thus, sequencing the ITS1\u20105.8S rDNA\u2010ITS2 region can accurately identify cryptic species with superior taxonomic resolution. Additionally, the MIC of amphotericin B against\n                    <jats:italic>A. stellatus<\/jats:italic>\n                    underscores the importance of precise molecular identification and antifungal MIC profiling in each case of pulmonary aspergillosis.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.70338","type":"journal-article","created":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T04:57:47Z","timestamp":1782104267000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Characterization and Antifungal Profiling of Nine Phenotypic\n                    <i>Aspergillus nidulans<\/i>\n                    Isolates: A Case Series From North India"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-0399-8505","authenticated-orcid":false,"given":"Aishwarya","family":"Nikhil","sequence":"first","affiliation":[{"name":"Mycology Research Group, Department of Microbiology, Institute of Medical Sciences Banaras Hindu University Varanasi Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atul Kumar","family":"Tiwari","sequence":"additional","affiliation":[{"name":"School of Chemistry Tel Aviv University Tel Aviv Yafo Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pearl","family":"Parashar","sequence":"additional","affiliation":[{"name":"Mycology Research Group, Department of Microbiology, Institute of Medical Sciences Banaras Hindu University Varanasi Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohit","family":"Bhatia","sequence":"additional","affiliation":[{"name":"Department of TB and Respiratory Medicine, Sir Sunderlal Hospital (BHU) Varanasi Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ragini","family":"Tilak","sequence":"additional","affiliation":[{"name":"Mycology Research Group, Department of Microbiology, Institute of Medical Sciences Banaras Hindu University Varanasi Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deepak","family":"Kumar","sequence":"additional","affiliation":[{"name":"Viral Research Diagnostic Laboratory, Department of Microbiology, Institute of Medical Science Banaras Hindu University Varanasi Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sudhir Kumar","family":"Singh","sequence":"additional","affiliation":[{"name":"Viral Research Diagnostic Laboratory, Department of Microbiology, Institute of Medical Science Banaras Hindu University Varanasi Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger J.","family":"Narayan","sequence":"additional","affiliation":[{"name":"Joint Department of Biomedical Engineering North Carolina State University Raleigh North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Munesh K.","family":"Gupta","sequence":"additional","affiliation":[{"name":"Mycology Research Group, Department of Microbiology, Institute of Medical Sciences Banaras Hindu University Varanasi Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,21]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.31018\/jans.v16i2.5449"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.2217\/17460913.1.4.435"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-10-421"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.simyco.2016.10.001"},{"key":"e_1_2_11_6_1","volume-title":"CLSI Document M38\u2010A2","author":"Clinical Laboratory Standards Institute","year":"2008"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myac072.S4.1d"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.3114\/sim.2007.59.01"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/13693780500064722"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hlife.2025.11.005"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/20499361241252537"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mycmed.2020.101004"},{"key":"e_1_2_11_13_1","unstructured":"Pa W. 2002. \u201cReference Method for Broth Dilution Antifungal Susceptibility Testing of Molds Approved Standard. CLSI Document M38\u2010A3.\u201d"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2022.09.013"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.simyco.2014.07.004"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/14787210.2024.2403017"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/00275514.2019.1698923"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof8111205"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/myc.13019"},{"key":"e_1_2_11_20_1","volume-title":"Larone's Medically Important Fungi: A Guide To Identification","author":"Westblade L. 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