{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T10:47:41Z","timestamp":1782470861871,"version":"3.54.5"},"reference-count":68,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T00:00:00Z","timestamp":1781568000000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The rapid emergence of multidrug resistant Enterobacterales, especially extended\u2010spectrum \u03b2\u2010lactamase (ESBL)\u2010producing strains, poses a significant One Health challenge in low\u2010 and middle\u2010income countries, including Bangladesh. This study aimed to investigate the prevalence, antimicrobial resistance patterns, and resistant genes profile of\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    and\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    isolated from quails in the Sylhet district of Bangladesh. A cross\u2010sectional study was conducted with 404 cloacal swabs were collected from quails across multiple retail locations. Isolates were identified using culture, biochemical tests, and PCR. Antimicrobial susceptibility was assessed by the Kirby\u2013Bauer disk diffusion method against 12 antibiotics. Resistance and virulence genes, including\n                    <jats:italic>stx1<\/jats:italic>\n                    ,\n                    <jats:italic>tetA<\/jats:italic>\n                    ,\n                    <jats:italic>strA<\/jats:italic>\n                    ,\n                    <jats:italic>AAC(3)\u2010iv<\/jats:italic>\n                    ,\n                    <jats:italic>sul1<\/jats:italic>\n                    , and\n                    <jats:italic>\u03b2\u2010<\/jats:italic>\n                    lactamase genes, were detected using multiplex and monoplex PCR. Multiple antibiotic resistance index (MARI) and MDR status were determined.\n                    <jats:italic>E. coli<\/jats:italic>\n                    was detected in 63.37% (256\/404) of samples, of which 53.91% were Shiga toxin\u2010producing (\n                    <jats:italic>stx1<\/jats:italic>\n                    ).\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    was confirmed in 11.14% (45) of isolates. Both organisms showed 100% resistance to ampicillin and amoxicillin\u2013clavulanic acid. Nearly all isolates were multidrug resistant, with mean MARI values of 0.58 for\n                    <jats:italic>E. coli<\/jats:italic>\n                    and 0.48 for\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    . High frequencies of resistance genes were observed, with strong phenotype\u2013genotype alignment. In\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    , the\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>TEM<\/jats:sub>\n                    (65.96%),\n                    <jats:italic>Multicase<\/jats:italic>\n                    <jats:sub>MOX<\/jats:sub>\n                    (44.68%) and\n                    <jats:italic>Multicase<\/jats:italic>\n                    <jats:sub>DHA<\/jats:sub>\n                    (10.63%) genes predominated. Overall, the findings highlight quails in Bangladesh as an important and previously under recognized reservoir of multidrug resistant\n                    <jats:italic>E. coli<\/jats:italic>\n                    and\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    , underscoring the urgent need for strengthened antimicrobial stewardship and One Health based surveillance strategies.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.70340","type":"journal-article","created":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T06:36:11Z","timestamp":1781678171000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Prevalence, Antimicrobial Resistance, and Resistance Gene Profiles of Extended\u2010Spectrum \u03b2\u2010Lactamase\u2010Producing\n                    <i>Escherichia coli<\/i>\n                    and\n                    <i>Klebsiella pneumoniae<\/i>\n                    Isolated From Quails in Sylhet, Bangladesh"],"prefix":"10.1002","volume":"15","author":[{"given":"Md. Al","family":"Mamun","sequence":"first","affiliation":[{"name":"Department of Medicine Sylhet Agricultural University Sylhet Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9785-2549","authenticated-orcid":false,"given":"Hemayet","family":"Hossain","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science and Animal Husbandry Teesta University Rangpur Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Shahidur Rahman","family":"Chowdhury","sequence":"additional","affiliation":[{"name":"Department of Medicine Sylhet Agricultural University Sylhet Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shahida Begum","family":"Nargis","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Sylhet Agricultural University, Bangladesh Sylhet Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Munni Rani","family":"Das","sequence":"additional","affiliation":[{"name":"Department of Medicine Sylhet Agricultural University Sylhet Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rakibul","family":"Hasan","sequence":"additional","affiliation":[{"name":"Department of Medicine Sylhet Agricultural University Sylhet Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nasrin Akter","family":"Liza","sequence":"additional","affiliation":[{"name":"Department of Medicine Sylhet Agricultural University Sylhet Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuvojit","family":"Mitra","sequence":"additional","affiliation":[{"name":"Department of Medicine Sylhet Agricultural University Sylhet Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0045-9669","authenticated-orcid":false,"given":"Md Bashir","family":"Uddin","sequence":"additional","affiliation":[{"name":"Department of Medicine Sylhet Agricultural University Sylhet Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8506-1202","authenticated-orcid":false,"given":"Md. Mukter","family":"Hossain","sequence":"additional","affiliation":[{"name":"Department of Medicine Sylhet Agricultural University Sylhet Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2062-8471","authenticated-orcid":false,"given":"Md. Mahfujur","family":"Rahman","sequence":"additional","affiliation":[{"name":"Department of Medicine Sylhet Agricultural University Sylhet Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,16]]},"reference":[{"issue":"3","key":"e_1_2_12_2_1","first-page":"537","article-title":"Isolation, Identification and Antibiogram Study of Bacteria Associated With Quail Feces in Sylhet Sadar, Bangladesh","volume":"31","author":"Al\u2010Amin M. 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