{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T10:46:48Z","timestamp":1782470808818,"version":"3.54.5"},"reference-count":84,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,6,21]],"date-time":"2026-06-21T00:00:00Z","timestamp":1782000000000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    is a major cause of hospital\u2010acquired infections and contributes to high mortality. We examined the molecular epidemiology, antibiotic resistance, and virulence features of\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    from the neonatal ward environment of a hospital in Chlef, Algeria, using whole\u2010genome sequencing (WGS). Antibiotic susceptibility was assessed with the Vitek 2 automated system (AST\u2010N365 card). Genomic DNA was extracted with the GF\u20101 kit, and WGS was performed by GENEWIZ Europe using the NEBNext Ultra II DNA Library Prep Kit. Species identification and virulence genes were determined using Kleborate, antimicrobial resistance genes were detected with AMRfinder Plus. From 9790 publicly available\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    genomes, the 112 closest matches were selected, and core\u2010genome MLST and a minimum\u2010spanning tree were generated in SeqSphere\u2009+\u2009v10.5.04 using a MLST and cgMLST scheme. All isolates were multidrug\u2010resistant, and WGS identified 22 resistance genes. Core genes detected across all samples included the\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX\u2010M\u201015<\/jats:sub>\n                    ,\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>TEM\u20101<\/jats:sub>\n                    ,\n                    <jats:italic>oqxA, oqxB, aph(6)\u2010Id, aph(3\u201d)\u2010Ib, sul2, fosA5, emrD<\/jats:italic>\n                    and\n                    <jats:italic>kdeA<\/jats:italic>\n                    . Other genes, including\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>SHV\u20101<\/jats:sub>\n                    ,\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>SHV\u201011<\/jats:sub>\n                    ,\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>DHA\u20101<\/jats:sub>\n                    ,\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>OXA\u20101<\/jats:sub>\n                    ,\n                    <jats:italic>qnrB4, qnrS1<\/jats:italic>\n                    and\n                    <jats:italic>aac(6\u2032)\u2010Ib\u2010cr5<\/jats:italic>\n                    were also observed. Yersiniabactin\u2010associated virulence genes\n                    <jats:italic>ybt<\/jats:italic>\n                    and\n                    <jats:italic>irp<\/jats:italic>\n                    were detected in KP32, KP37 and KP57 (high risk clone ST13) while\n                    <jats:italic>qacE<\/jats:italic>\n                    was present only in KP55 (novel strain ST8932). This study highlights the persistence of\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX\u2010M\u201015<\/jats:sub>\n                    ,\n                    <jats:italic>qnr<\/jats:italic>\n                    , and\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>DHA\u20101<\/jats:sub>\n                    in\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    from the neonatal ward environment. The presence of the high\u2010risk strain ST13 is particularly concerning, as its identical allelic profiles indicate likely clonal spread within the hospital. These results suggest the hospital environment may act as a reservoir for multidrug\u2010resistant\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1002\/mbo3.70346","type":"journal-article","created":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T04:49:42Z","timestamp":1782103782000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Role of Neonatal Ward Environment as a Reservoir for the Dissemination of Multidrug\u2010Resistant\n                    <i>Klebsiella pneumoniae<\/i>\n                    in Algeria"],"prefix":"10.1002","volume":"15","author":[{"given":"Meriem","family":"Derkaoui","sequence":"first","affiliation":[{"name":"Department of Biology, Laboratory of Molecular Biology, Genomics and Bioinformatics, Faculty of Nature and Life Sciences University Hassiba Benbouali Chlef Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin Alexander","family":"Fischer","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Division of Nosocomial Pathogens and Antimicrobial Resistances Robert Koch Institute  Wernigerode Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed","family":"Sebaihia","sequence":"additional","affiliation":[{"name":"Department of Biology, Laboratory of Molecular Biology, Genomics and Bioinformatics, Faculty of Nature and Life Sciences University Hassiba Benbouali Chlef Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachida","family":"Namoune","sequence":"additional","affiliation":[{"name":"Department of Biology, Laboratory of Molecular Biology, Genomics and Bioinformatics, Faculty of Nature and Life Sciences University Hassiba Benbouali Chlef Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatiha","family":"Bouheudjeur","sequence":"additional","affiliation":[{"name":"Department of Biology, Laboratory of Molecular Biology, Genomics and Bioinformatics, Faculty of Nature and Life Sciences University Hassiba Benbouali Chlef Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meryem","family":"Beloukarif","sequence":"additional","affiliation":[{"name":"Department of Biology, Laboratory of Molecular Biology, Genomics and Bioinformatics, Faculty of Nature and Life Sciences University Hassiba Benbouali Chlef Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bakhta","family":"Madaoui","sequence":"additional","affiliation":[{"name":"Public Hospital Establishment  Chlef Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1476-2108","authenticated-orcid":false,"given":"Bruno Silvester","family":"Lopes","sequence":"additional","affiliation":[{"name":"School of Health and Life Sciences, Life Sciences Teesside University  Middlesbrough UK"},{"name":"National Horizons Centre Teesside University Darlington UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,21]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0308523"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2023.10.010"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1179\/1973947813Y.0000000115"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3855\/jidc.6727"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22084042"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciab784"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1136\/archdischild-2022-324476"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000035638"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2013.0193"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2016.08.011"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2019.04.005"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.01070"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/msystems.00206-23"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2016.07.013"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-91456-0"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2023.05.018"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2019.0289"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-024-10344-w"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13756-017-0182-3"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cix270"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt086"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics13020127"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.3947\/ic.2022.0117"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0224065"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/msystems.00972-23"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(22)02185-7"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00036-08"},{"key":"e_1_2_11_29_1","doi-asserted-by":"crossref","unstructured":"Jeli\u0107 M. J.Hrenovi\u0107 S.Deki\u0107 I.Goi\u0107\u2010Bari\u0161i\u0107 andA.Tambi\u0107 Andra\u0161evi\u0107First Evidence of KPC\u2010Producing ST258 Klebsiella pneumoniae in River Water.103 147\u2013150(2019).","DOI":"10.1016\/j.jhin.2019.04.001"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2001.tb10762.x"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-023-08086-2"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0295286"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2022.0254"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-24448-3"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aah6500"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(15)00474-2"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.3855\/jidc.18907"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1080\/14767058.2024.2327828"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2011.03570.x"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkz146"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiph.2023.12.014"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpz1.323"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1093\/jacamr\/dlae050"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268812002099"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00261-16"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1178547"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2016.0153"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1179\/2047773214Y.0000000136"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms10020416"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(21)02724-0"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-016-1580-5"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.21037\/tp-2025-57"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics14020128"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-46684-z"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.3390\/idr13010025"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-016-0997-x"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2024.1328123"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2023.02.011"},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.17219\/acem\/94165"},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.3855\/jidc.17168"},{"key":"e_1_2_11_61_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules22122267"},{"key":"e_1_2_11_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2009.02.004"},{"key":"e_1_2_11_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2024.01.009"},{"key":"e_1_2_11_64_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv681"},{"key":"e_1_2_11_65_1","article-title":"MOB\u2010Suite: Software Tools for Clustering, Reconstruction and Typing of Plasmids From Draft Assemblies","volume":"4","year":"2018","journal-title":"Microbial Genomics"},{"key":"e_1_2_11_66_1","doi-asserted-by":"publisher","DOI":"10.1080\/09603123.2021.1885631"},{"key":"e_1_2_11_67_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0187832"},{"key":"e_1_2_11_68_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-021-00870-7"},{"key":"e_1_2_11_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(25)00118-5"},{"key":"e_1_2_11_70_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13756-020-00721-w"},{"key":"e_1_2_11_71_1","doi-asserted-by":"publisher","DOI":"10.20344\/amp.15174"},{"key":"e_1_2_11_72_1","doi-asserted-by":"publisher","DOI":"10.1051\/vetres:2001123"},{"key":"e_1_2_11_73_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-025-12120-w"},{"key":"e_1_2_11_74_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-17996-1"},{"key":"e_1_2_11_75_1","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2007.715"},{"key":"e_1_2_11_76_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2021.02.018"},{"key":"e_1_2_11_77_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms12010136"},{"key":"e_1_2_11_78_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2024.151601"},{"key":"e_1_2_11_79_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1005595"},{"key":"e_1_2_11_80_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41429-021-00413-6"},{"key":"e_1_2_11_81_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2019.06.004"},{"key":"e_1_2_11_82_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph19106039"},{"key":"e_1_2_11_83_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0254805"},{"key":"e_1_2_11_84_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2017.05.015"},{"key":"e_1_2_11_85_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2023.114965"}],"container-title":["MicrobiologyOpen"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mbo3.70346","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/mbo3.70346","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mbo3.70346","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T09:50:55Z","timestamp":1782467455000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mbo3.70346"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":84,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1002\/mbo3.70346"],"URL":"https:\/\/doi.org\/10.1002\/mbo3.70346","archive":["Portico"],"relation":{},"ISSN":["2045-8827","2045-8827"],"issn-type":[{"value":"2045-8827","type":"print"},{"value":"2045-8827","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6]]},"assertion":[{"value":"2026-02-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70346"}}