{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:45:22Z","timestamp":1791333922622,"version":"4.3.1"},"reference-count":62,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T00:00:00Z","timestamp":1782172800000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Extended\u2010spectrum beta\u2010lactamase\u2010producing\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    (ESBL\u2010KP) and carbapenem\u2010resistant\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    (CRKP) represent a global health risk, with ornamental birds serving as potential reservoirs for their dissemination. This study aimed to isolate and characterize ESBL\u2010KP and CRKP from ornamental birds and their surrounding environments. A total of 112 samples, including equal numbers of feces, cage swab, cage water, cage feed, store feed, handler's hand swab, nasal swab, and air samples, were collected equally from both households and pet bird shops. Overall, 52.68% (59\/112, CI\n                    <jats:sub>95<\/jats:sub>\n                    : 0.435\u20130.6168) isolates were confirmed as positive, where a higher occurrence was observed in pet store samples (32\/56, 57.14%) than in household samples (27\/56, 48.21%). In the disk diffusion test, 79.67% (47\/59, CI\n                    <jats:sub>95<\/jats:sub>\n                    : 0.6773\u20130.8796) isolates were identified as multidrug\u2010resistant, with high resistance to imipenem and cefotaxime (both 66.10%), meropenem and ceftriaxone (each 59.02%). The occurrence of ESBL\u2010producing isolates was higher in shop samples (26\/32, 81.25%) than in household samples (12\/27, 44.44%). In the Congo Red assay, 76.28% (45\/59, CI\n                    <jats:sub>95<\/jats:sub>\n                    : 0.6403\u20130.8531) isolates were identified as strong biofilm producers. Resistance genes,\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX\u2010M<\/jats:sub>\n                    (92.10%),\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>TEM<\/jats:sub>\n                    (84.21%),\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>SHV<\/jats:sub>\n                    (78.95%),\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>OXA\u201051<\/jats:sub>\n                    (51.42%), and\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>NDM<\/jats:sub>\n                    (34.29%), conferring resistance to beta\u2010lactams and carbapenems were detected in the\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    isolates, along with virulence genes,\n                    <jats:italic>uge<\/jats:italic>\n                    (100%),\n                    <jats:italic>entB<\/jats:italic>\n                    (83.05%),\n                    <jats:italic>mrkD<\/jats:italic>\n                    (81.36%), and\n                    <jats:italic>kfu<\/jats:italic>\n                    (30.51%). As a first report from Bangladesh, this study highlights the widespread occurrence of ESBL\u2010KP, CRKP and biofilm\u2010producing\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    in ornamental birds, emphasizing the urgent need for a One Health approach to prevent associated zoonotic and environmental risks.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.70348","type":"journal-article","created":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T04:10:03Z","timestamp":1782274203000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Detection and Characterization of ESBL\u2010Producing and Carbapenem\u2010Resistant\n                    <i>Klebsiella pneumoniae<\/i>\n                    in Ornamental Birds and Their Surrounding Environments"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-2789-3947","authenticated-orcid":false,"given":"Md Abdullah Evna","family":"Hasan","sequence":"first","affiliation":[{"name":"Department of Microbiology and Hygiene, Faculty of Veterinary Science Bangladesh Agricultural University Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1400-1250","authenticated-orcid":false,"given":"Md. Liton","family":"Rana","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hygiene, Faculty of Veterinary Science Bangladesh Agricultural University Mymensingh Bangladesh"},{"name":"National Engineering Research Center of Industrial Wastewater Detoxication and Resource Recovery, Research Center for Eco\u2010Environmental Sciences Chinese Academy of Sciences Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6870-4595","authenticated-orcid":false,"given":"Md Saiful","family":"Islam","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hygiene, Faculty of Veterinary Science Bangladesh Agricultural University Mymensingh Bangladesh"},{"name":"Department of Animal Science University of California \u2010 Davis Davis California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sadia Afrin","family":"Punom","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hygiene, Faculty of Veterinary Science Bangladesh Agricultural University Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Tabeer Hossain","family":"Antor","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hygiene, Faculty of Veterinary Science Bangladesh Agricultural University Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diruba Akter","family":"Jany","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hygiene, Faculty of Veterinary Science Bangladesh Agricultural University Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naeem Ahammed Ibrahim","family":"Fahim","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hygiene, Faculty of Veterinary Science Bangladesh Agricultural University Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S M","family":"Abu Sama Al Faruquee","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hygiene, Faculty of Veterinary Science Bangladesh Agricultural University Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Raisul","family":"Islam","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hygiene, Faculty of Veterinary Science Bangladesh Agricultural University Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuhayr","family":"Bakhtiyar","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hygiene, Faculty of Veterinary Science Bangladesh Agricultural University Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anindita Ash","family":"Prome","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hygiene, Faculty of Veterinary Science Bangladesh Agricultural University Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. 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