{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T08:21:16Z","timestamp":1787732476290,"version":"build-2784847793"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T00:00:00Z","timestamp":1783209600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T00:00:00Z","timestamp":1783209600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      Carbapenem\u2010resistant\n                      <jats:italic>Acinetobacter baumannii<\/jats:italic>\n                      (CRAB) has been designated a top\u2010priority critical pathogen by the World Health Organization, and the CDC has also classified it as a serious and immediate public health threat. The Cross\u2010sectional study was conducted to detect the\n                      <jats:italic>\n                        <jats:sub>bla<\/jats:sub>\n                      <\/jats:italic>\n                      <jats:sub>OXA\u201023<\/jats:sub>\n                      gene among carbapenem\u2010resistant\n                      <jats:italic>Acinetobacter baumannii<\/jats:italic>\n                      isolated from clinical specimens in intensive care units. The study was conducted at the Department of Microbiology, Institute of Pathology and Diagnostic Medicine, Khyber Medical University, from January 2024 to December 2024. Phenotypically confirmed Carbapenem\u2010resistant\n                      <jats:italic>Acinetobacter baumannii<\/jats:italic>\n                      isolates were genotypically verified via\n                      <jats:sub>\n                        <jats:italic>bla<\/jats:italic>\n                        OXA\u201051\n                      <\/jats:sub>\n                      detection, then screened for\n                      <jats:sub>\n                        <jats:italic>bla<\/jats:italic>\n                        OXA\u201023\n                      <\/jats:sub>\n                      using polymerase chain reaction, and PCR mapping was done for the upstream insertion of\n                      <jats:italic>ISAba1<\/jats:italic>\n                      .\n                      <jats:sub>\n                        <jats:italic>bla<\/jats:italic>\n                        OXA\u201023\n                      <\/jats:sub>\n                      gene with an upstream insertion of\n                      <jats:italic>ISAba1<\/jats:italic>\n                      is detected in all 52 (100%) isolates. ST19 is the most commonly identified ST, accounting for 31 (59.6%), followed by ST2 14 (26.9%) and ST1 7 (13.4%). Consequently, these findings emphasize the implementation of targeted infection control measures and routine surveillance screening within high\u2010risk ICU environments to mitigate further transmission.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/mbo3.70364","type":"journal-article","created":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T04:34:38Z","timestamp":1783312478000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Detection of\n                    <sub>OXA\u201023<\/sub>\n                    Among Carbapenem\u2010Resistant\n                    <i>Acinetobacter baumannii<\/i>\n                    Isolated From Intensive Care Units of a Tertiary Care Hospital in Pakistan"],"prefix":"10.1002","volume":"15","author":[{"given":"Hafsa","family":"Waseem","sequence":"first","affiliation":[{"name":"Institute of Pathology and Diagnostic Medicine (IPDM), Khyber Medical University Peshawar Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3737-2321","authenticated-orcid":false,"given":"Ihsan","family":"Ullah","sequence":"additional","affiliation":[{"name":"Institute of Pathology and Diagnostic Medicine (IPDM), Khyber Medical University Peshawar Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3953-593X","authenticated-orcid":false,"given":"Shahzad","family":"Ahmad","sequence":"additional","affiliation":[{"name":"School of Medicine, The University of Buckingham Buckingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irfan Ali","family":"Mirza","sequence":"additional","affiliation":[{"name":"Armed Forces Postgraduate Medical Institute (AFPGMI) Rawalpindi Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benish","family":"Aleem","sequence":"additional","affiliation":[{"name":"Institute of Pathology and Diagnostic Medicine (IPDM), Khyber Medical University Peshawar Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shazia","family":"Iqbal","sequence":"additional","affiliation":[{"name":"School of Medicine, The University of Buckingham Buckingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,5]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0317798"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-025-11169-x"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.3855\/jidc.19119"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S459739"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.9.4382-4390.2005"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciad109"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.4103\/0253-7176.116232"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11756-022-01119-2"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S310478"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0251508"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.1045206"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0041-1740019"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms13091983"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2020.01.001"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.5145\/ACM.2024.27.2.8"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2024.1338395"},{"issue":"3","key":"e_1_2_13_18_1","first-page":"18","article-title":"Antibiotic Resistance Patterns and Detection of OXA\u201023 and OXA\u201048 Genes in Acinetobacter baumannii Isolated From Ventilator\u2010Associated Pneumonia","volume":"8","author":"Lari A. 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