{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T08:20:47Z","timestamp":1787732447389,"version":"build-2784847793"},"reference-count":72,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,7,19]],"date-time":"2026-07-19T00:00:00Z","timestamp":1784419200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,19]],"date-time":"2026-07-19T00:00:00Z","timestamp":1784419200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study focused on biological activities and chemical characterization of secondary metabolites from the fungus\n                    <jats:italic>Talaromyces annesophieae<\/jats:italic>\n                    MD2 (GenBank: PQ252671). The dichloromethane extract (DCM\u2010E) of its culture supernatant was fractionated using chromatographic techniques. Initially, three main fractions were first prepared from DCM\u2010E. Subsequently, the selected active fraction 2 was subfractionated into six subfractions. The subfraction 3 demonstrated high water solubility and strong antibacterial activity against both gram\u2010positive and gram\u2010negative bacteria (MIC values of 105.41, 82.76, 71.92, and 70.38\u2009\u00b5g\/mL against\n                    <jats:italic>B. cereus, S. aureus, E. coli<\/jats:italic>\n                    , and\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    , respectively). It exhibited selective anticancer activity through regulation of autophagy\u2010 and apoptosis\u2010related pathways without affecting healthy cells (IC\n                    <jats:sub>50<\/jats:sub>\n                    values of 7.65, 3.76, 10.76\u2009\u00b5g\/mL against HT\u201029, DU\u2010145 and SH\u2010SY5Y, respectively). Furthermore, it exhibited DPPH and ABTS radical\u2010scavenging activities (48.3% and 44.1% at the concentration of 100\u2009\u03bcg\/mL, respectively) and protected dermal fibroblasts against H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    \u2013induced toxicity. Structural analyses elucidated that the subfraction 3 contained four nitrogenous polar compounds. Three compounds (C\n                    <jats:sub>5<\/jats:sub>\n                    H\n                    <jats:sub>3<\/jats:sub>\n                    O\n                    <jats:sub>4<\/jats:sub>\n                    N\n                    <jats:sub>8<\/jats:sub>\n                    , C\n                    <jats:sub>15<\/jats:sub>\n                    H\n                    <jats:sub>25<\/jats:sub>\n                    O\n                    <jats:sub>4<\/jats:sub>\n                    N\n                    <jats:sub>4,<\/jats:sub>\n                    and C\n                    <jats:sub>23<\/jats:sub>\n                    H\n                    <jats:sub>21<\/jats:sub>\n                    N\n                    <jats:sub>4<\/jats:sub>\n                    ) were suggested to be alkaloid\u2010like or nitrogen\u2010rich heterocyclic secondary metabolites, while the remaining compound (C\n                    <jats:sub>9<\/jats:sub>\n                    H\n                    <jats:sub>9<\/jats:sub>\n                    O\n                    <jats:sub>4<\/jats:sub>\n                    N) was probably an aminobenzoic acid\u2010type or heteroaromatic carboxylic acid derivative. This is the first report on bioactive metabolites of\n                    <jats:italic>T. annesophieae<\/jats:italic>\n                    . Due to multifunctional biological activities, its metabolites can find application in dermocosmetics and pharmacology.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.70367","type":"journal-article","created":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T06:43:33Z","timestamp":1784529813000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Water Soluble\u2010Nitrogenous Secondary Metabolites From\n                    <i>Talaromyces annesophieae<\/i>\n                    MD2 Exhibit Anti\u2010Bacterial, Anti\u2010Cancer, Anti\u2010Oxidant, and Cytoprotective Activities"],"prefix":"10.1002","volume":"15","author":[{"given":"Meryem","family":"Doymus\u2010Yilmaz","sequence":"first","affiliation":[{"name":"Vocational School of Health Services of Hinis Ataturk University Erzurum T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huseyin","family":"Aksit","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, Faculty of Pharmacy Erzincan Binali Yildirim University Erzincan T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9350-9628","authenticated-orcid":false,"given":"Mesut","family":"Taskin","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics, Faculty of Science Ataturk University Erzurum T\u00fcrkiye"},{"name":"Institute of Medicine, Vaccine and Biotechnology Ataturk University Erzurum T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,19]]},"reference":[{"issue":"4","key":"e_1_2_10_2_1","first-page":"2021","article-title":"Identification and Evaluation of Antimicrobial and Cytotoxic Activities of Penicillium Islandicum and Aspergillus tamarii Ethyle Acetate Extracts","volume":"6","author":"Abdel\u2010Hady H.","year":"2016","journal-title":"Pharmaceuticals"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/bbb.70099"},{"key":"e_1_2_10_4_1","first-page":"966","volume-title":"Molecular Biology of the Cell","author":"Alberts B.","year":"1994"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/md20050326"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/md20030217"},{"key":"e_1_2_10_7_1","first-page":"1189","article-title":"Natural Phenolic Compounds: A Potential Antifungal Agent","volume":"1","author":"Ansari M. 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