{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T08:21:05Z","timestamp":1787732465518,"version":"build-2784847793"},"reference-count":89,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Macroalgae and their associated microbes form tightly integrated holobionts, yet cross\u2010study comparisons of epiphytic microbiomes remain constrained by heterogeneous sampling designs, primer selection, and analytical pipelines. We performed a harmonized meta\u2010analysis of publicly available 16S rRNA gene datasets to examine diversity, community structure, and inferred functional profiles within a common analytical framework. A structured search identified 16 studies, totaling 763 samples, from macroalgae and adjacent seawater across four oceanic regions. All datasets were reprocessed using a standardized workflow to ensure cross\u2010study comparability. The compiled dataset was strongly skewed towards brown macroalgae (Phaeophyceae) and the V4 region of the 16S gene, highlighting both the prominence of Laminariales in current research and the need for methodological harmonization. Within this framework, alpha diversity differed among macroalgal orders, with Fucales hosting the richest epiphytic assemblages, Desmarestiales intermediate diversity, and Laminariales the lowest, while seawater exhibited intermediate values. Community composition was structured by host taxonomic identity and sample type. Within Laminariales, thalli supported assemblages distinct from seawater, sharing a limited taxonomic core and displaying reproducible genus\u2010level signatures. Trait\u2010based functional annotation indicated enrichment of aerobic chemoheterotrophy and polysaccharide\u2010associated metabolisms in Laminariales communities, whereas seawater showed greater representation of nitrogen\u2010 and sulfur\u2010related processes. These findings demonstrate that cross\u2010study reprocessing resolves consistent host\u2010associated patterns and establishes a quantitative framework for future multi\u2010omics and experimental investigations.<\/jats:p>","DOI":"10.1002\/mbo3.70372","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:56:31Z","timestamp":1787032591000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Standardized 16S Meta\u2010Analysis Reveals Consistent Host\u2010Associated Patterns in Laminariales Epiphytic Microbiomes"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6114-6650","authenticated-orcid":false,"given":"J. Z.","family":"Florez","sequence":"first","affiliation":[{"name":"Centro i~mar, CeBiB and MASH Universidad de Los Lagos  Puerto Montt Chile"},{"name":"Departamento de Ciencias y Geograf\u00eda, Facultad de Ciencias Naturales y Exactas and HUB Ambiental UPLA Universidad de Playa Ancha  Valpara\u00edso Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3803-1430","authenticated-orcid":false,"given":"A.","family":"Z\u00e1rate","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias de la Salud, Instituto de Ciencias Aplicadas Universidad Aut\u00f3noma de Chile  Temuco Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6327-1785","authenticated-orcid":false,"given":"P.","family":"Bruna","sequence":"additional","affiliation":[{"name":"N\u00facleo Cient\u00edfico y Tecnol\u00f3gico en Biorecursos (BIOREN) Universidad de La Frontera  Temuco Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6362-3529","authenticated-orcid":false,"given":"B.","family":"Leyton\u2010Carcaman","sequence":"additional","affiliation":[{"name":"N\u00facleo Cient\u00edfico y Tecnol\u00f3gico en Biorecursos (BIOREN) Universidad de La Frontera  Temuco Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9090-1344","authenticated-orcid":false,"given":"V.","family":"Molina","sequence":"additional","affiliation":[{"name":"Departamento de Ciencias y Geograf\u00eda, Facultad de Ciencias Naturales y Exactas and HUB Ambiental UPLA Universidad de Playa Ancha  Valpara\u00edso Chile"},{"name":"Centro de Investigaci\u00f3n Oceanogr\u00e1fica COPAS COASTAL Universidad de Concepci\u00f3n  Concepci\u00f3n Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2435-8504","authenticated-orcid":false,"given":"M. B.","family":"Hengst","sequence":"additional","affiliation":[{"name":"Departamento de Ciencias Farmac\u00e9uticas, Facultad de Ciencias Universidad Cat\u00f3lica del Norte  Antofagasta Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6929-6514","authenticated-orcid":false,"given":"C.","family":"Camus","sequence":"additional","affiliation":[{"name":"Centro i~mar, CeBiB and MASH Universidad de Los Lagos  Puerto Montt Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9136-0041","authenticated-orcid":false,"given":"M.","family":"Abanto","sequence":"additional","affiliation":[{"name":"N\u00facleo Cient\u00edfico y Tecnol\u00f3gico en Biorecursos (BIOREN) Universidad de La Frontera  Temuco Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5344-054X","authenticated-orcid":false,"given":"L.","family":"Barrientos","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias de la Salud, Instituto de Ciencias Aplicadas Universidad Aut\u00f3noma de Chile  Temuco Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3246-681X","authenticated-orcid":false,"given":"A. H.","family":"Buschmann","sequence":"additional","affiliation":[{"name":"Centro i~mar, CeBiB and MASH Universidad de Los Lagos  Puerto Montt Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,17]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.01202-20"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsec\/fiae160"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.b.22931"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-018-0024-1"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2011.119"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1609\/icwsm.v3i1.13937"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2014.00219"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbz155"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-019-0209-9"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1101591108"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/09670262.2017.1376709"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3869"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2486.2011.02456.x"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2018.1887"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.839465"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.10911"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/1574-6976.12011"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-91955-9_1"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2832"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/07388551.2021.1922351"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.02561"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.algal.2019.101706"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSystems.00217-17"},{"key":"e_1_2_11_25_1","volume-title":"Algae","author":"Graham & Wilcox","year":"1999"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0146307"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-444-64192-2.00016-0"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1529-8817.2012.01224.x"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.algal.2024.103656"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsmc\/xtad023"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-018-0029-9"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpy.12394"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0709959105"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1080\/08927010903474189"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-540-74181-7_11"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2016.11.008"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.13993"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2019.03.002"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf4507"},{"key":"e_1_2_11_40_1","first-page":"936","article-title":"Function and Functional Redundancy in Microbial Systems. Nat","volume":"2","author":"Louca S.","year":"2018","journal-title":"Ecology and Evolution"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-020-00857-2"},{"key":"e_1_2_11_42_1","first-page":"1","volume-title":"Symbiosis as a source of evolutionary innovation: Speciation and morphogenesis","author":"Margulis L.","year":"1991"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1341"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12972"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpy.13485"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-023-01845-1"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.3390\/md18120641"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01603792"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0192772"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1128\/msystems.00592-22"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0053608"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.01800"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01240"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-69362-y"},{"key":"e_1_2_11_55_1","first-page":"1","article-title":"Interactions Within the Microbiome Alter Microbial Interactions With Host Chemical Defences and Affect Disease in a Marine Holobiont","volume":"9","author":"Nielsen S.","year":"2019","journal-title":"Scientific Reports"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.00494"},{"key":"e_1_2_11_57_1","volume-title":"Proceedings of the Colloquium on Conservation Problems in Antarctica Lawrence","author":"Paul K. D.","year":"1972"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/218776a0"},{"key":"e_1_2_11_59_1","doi-asserted-by":"crossref","unstructured":"Priest T. N.Henry T.Weber et al.2025. \u201cThe Jedi Marker as a Universal Measure of Planetary Biodiversity.\u201d Preprint bioRxiv August 13.https:\/\/doi.org\/10.1101\/2025.08.11.669668.","DOI":"10.1101\/2025.08.11.669668"},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/1751-7915.14014"},{"key":"e_1_2_11_61_1","doi-asserted-by":"publisher","DOI":"10.1080\/08927014.2015.1041020"},{"key":"e_1_2_11_62_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12352"},{"key":"e_1_2_11_63_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.14529"},{"key":"e_1_2_11_64_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fuy030"},{"key":"e_1_2_11_65_1","first-page":"1","article-title":"A Review on the Chemical Ecology of the Fucaceae Holobionts: From Fundamental Knowledge to Applications","volume":"26","author":"Rousseau C.","year":"2024","journal-title":"Comptes Rendus Chimie"},{"key":"e_1_2_11_66_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40793-025-00825-z"},{"key":"e_1_2_11_67_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.20018"},{"key":"e_1_2_11_68_1","doi-asserted-by":"publisher","DOI":"10.1111\/1365-2745.13193"},{"key":"e_1_2_11_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.csbj.2020.01.005"},{"key":"e_1_2_11_70_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-62094-7_15"},{"key":"e_1_2_11_71_1","doi-asserted-by":"publisher","DOI":"10.1111\/1574-6941.12297"},{"key":"e_1_2_11_72_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.01488"},{"key":"e_1_2_11_73_1","doi-asserted-by":"publisher","DOI":"10.1128\/msystems.00002-23"},{"key":"e_1_2_11_74_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.16564"},{"key":"e_1_2_11_75_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature24621"},{"key":"e_1_2_11_76_1","doi-asserted-by":"publisher","DOI":"10.1038\/sdata.2017.203"},{"key":"e_1_2_11_77_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41597-023-02877-7"},{"key":"e_1_2_11_78_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-69538-6"},{"key":"e_1_2_11_79_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2012.00292"},{"key":"e_1_2_11_80_1","doi-asserted-by":"publisher","DOI":"10.1127\/pip\/2015\/0019"},{"key":"e_1_2_11_81_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00062-07"},{"key":"e_1_2_11_82_1","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbz105"},{"key":"e_1_2_11_83_1","article-title":"FIGARO: An Efficient and Objective Tool for Optimizing Microbiome rRNA Gene Trimming Parameters","author":"Weinstein M. M.","year":"2019","journal-title":"BioRxiv"},{"key":"e_1_2_11_84_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-017-0237-y"},{"key":"e_1_2_11_85_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2015.00086"},{"key":"e_1_2_11_86_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsle\/fnz152"},{"key":"e_1_2_11_87_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.16378"},{"key":"e_1_2_11_88_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2008.00123.x"},{"key":"e_1_2_11_89_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00146"},{"key":"e_1_2_11_90_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.9143"}],"container-title":["MicrobiologyOpen"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mbo3.70372","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/mbo3.70372","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mbo3.70372","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T07:54:11Z","timestamp":1787730851000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mbo3.70372"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":89,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/mbo3.70372"],"URL":"https:\/\/doi.org\/10.1002\/mbo3.70372","archive":["Portico"],"relation":{},"ISSN":["2045-8827","2045-8827"],"issn-type":[{"value":"2045-8827","type":"print"},{"value":"2045-8827","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"2026-03-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70372"}}