{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T08:21:08Z","timestamp":1787732468612,"version":"build-2784847793"},"reference-count":84,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100010747","name":"Consejo Nacional de Ciencia, Tecnolog\u00eda e Innovaci\u00f3n Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["PE501088097-2024-PROCIENCIA"],"award-info":[{"award-number":["PE501088097-2024-PROCIENCIA"]}],"id":[{"id":"10.13039\/501100010747","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Polyethylene terephthalate (PET) waste represents a major environmental challenge due to limited recycling solutions. Thermophilic bacteria from geothermal environments harbor diverse enzymatic machinery adapted to extreme conditions, offering promising biocatalysts for plastic degradation; however, biological resources from Peru and other South American countries remain scarce. We characterized four bacterial strains isolated from two geothermal sites in Cajamarca, Peru, screened for PET hydrolysis at 50\u00b0C. Whole\u2010genome sequencing using hybrid assembly achieved near\u2010complete circular genomes. GTDB\u2010Tk classification identified three species:\n                    <jats:italic>Neobacillus thermocopriae<\/jats:italic>\n                    (strain 19A),\n                    <jats:italic>Bacillus licheniformis<\/jats:italic>\n                    (strains 16P and BI2), and\n                    <jats:italic>Brevibacillus agri<\/jats:italic>\n                    (strain BI8). Quantitative assays revealed that strain 16P achieved the highest mass loss (0.598%), followed by strain BI8 (0.449%). ATR\u2010FTIR analysis of the incubated sheets showed a significant reduction of the ester carbonyl index in strains 16P, 19A, and BI8 relative to both non\u2010incubated PET and an abiotic control, whereas strain BI2 did not differ from the controls, indicating preferential modification of ester bonds at the sheet surface. Genome mining and structure\u2010based homology searches identified multiple candidate enzymes similar to validated PETases and carboxylesterases, including PETase46\u2010like homologs in strains BI8 and 16P and a terephthalate\u2010active carboxylesterase homolog in strain 16P. Molecular docking supported the conservation of catalytic geometry and substrate\u2010binding sites in these candidates. This work represents one of the first systematic genomic and structural characterizations of putative PET\u2010hydrolases in Peruvian geothermal bacteria, expanding knowledge of extremophile diversity and advancing thermostable enzymes for sustainable plastic waste management.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.70376","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T04:44:36Z","timestamp":1786682676000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Experimental, Genomic, and Structural Evidence Supporting Putative PET\u2010Hydrolases in Thermophilic Bacteria Isolated From Hot Springs in Cajamarca, Peru"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9046-5359","authenticated-orcid":false,"given":"Marco A.","family":"Rivera\u2010Jacinto","sequence":"first","affiliation":[{"name":"Laboratorio de Microbiolog\u00eda, 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