{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T11:37:07Z","timestamp":1793101027227,"version":"4.5.0"},"reference-count":92,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study aimed to assess the genetic diversity and potential epidemiological overlap of\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    using molecular (spa and SCCmec typing) and phenotypic characterization of 108 isolates obtained along the farm\u2010to\u2010fork continuum (dairy and meat chains) and 50 human clinical isolates. Forty\u2010four\n                    <jats:italic>spa<\/jats:italic>\n                    types, including 17 novel patterns, were identified, with t11284 and t127 predominating among animal\u2010related MRSA and clinical MRSA, respectively. Six SCCmec types (I\u2013VI) were detected in the majority of isolates (85.2%), with SCCmec IVa prevalent in farm\u2010to\u2010fork isolates (67%) and SCCmec III dominant in clinical isolates (28%). Spa repeat\u2010based MST analysis revealed a heterogeneous distribution of isolates across clusters, with identical spa types detected in multiple source categories, indicating genetic relatedness rather than direct transmission events. Overall, 54.6% of isolates exhibited a multidrug\u2010resistant phenotype. Farm\u2010to\u2010fork isolates showed mainly \u03b2\u2010lactam resistance (\u2265\u200985%), whereas clinical MRSA exhibited broader resistance profiles, including high fluoroquinolone resistance (\u2265\u200992%). PVL was detected in 41 isolates (38%), predominantly in MRSA, and was associated with SCCmec IV\/V and diverse spa types. Toxin genes (\n                    <jats:italic>tst<\/jats:italic>\n                    \u22121,\n                    <jats:italic>sea<\/jats:italic>\n                    ,\n                    <jats:italic>seb<\/jats:italic>\n                    , and\n                    <jats:italic>sed<\/jats:italic>\n                    ) were mainly confined to clinical MRSA, suggesting source\u2010associated distribution of virulence determinants. Biofilm formation was observed in 49 isolates (45.3%), more frequently among farm\u2010to\u2010fork isolates. Our study demonstrate marked genetic and phenotypic diversity of\n                    <jats:italic>S. aureus<\/jats:italic>\n                    across farm\u2010to\u2010fork and human clinical sources and suggest the presence of shared genetic lineages among isolates from different sources. The results support the importance of integrated One Health surveillance for monitoring antimicrobial\u2010resistant and virulent\n                    <jats:italic>S. aureus<\/jats:italic>\n                    populations across interconnected ecological compartments.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.70397","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T04:20:14Z","timestamp":1788495614000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genetic Diversity and Epidemiological Overlap of\n                    <i>Staphylococcus aureus<\/i>\n                    at the Animal\u2013Food\u2013Environment\u2013Human Interface Within a One Health Framework"],"prefix":"10.1002","volume":"15","author":[{"given":"Pinar","family":"Sagiroglu","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine Erciyes University Kayseri T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dursun Alp","family":"Gundog","sequence":"additional","affiliation":[{"name":"Veterinary Faculty, Department of Food Hygiene and Technology Yozgat Bozok University Yozgat T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Candan","family":"Gungor","sequence":"additional","affiliation":[{"name":"Department of Veterinary Food Hygiene and Technology, Faculty of Veterinary Medicine Necmettin Erbakan University Ere\u011fli T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kursat","family":"Koskeroglu","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health, Faculty of Veterinary Medicine Erciyes University Kayseri T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4169-0637","authenticated-orcid":false,"given":"Mustafa Altay","family":"Atalay","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine Erciyes University Kayseri T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adalet","family":"Dishan","sequence":"additional","affiliation":[{"name":"Veterinary Faculty, Department of Food Hygiene and Technology Yozgat Bozok University Yozgat T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8783-3889","authenticated-orcid":false,"given":"Yeliz","family":"Ucar","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health, Faculty of Veterinary Medicine Erciyes University Kayseri T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aytac","family":"Akcay","sequence":"additional","affiliation":[{"name":"Department of Biostatistics Ankara University, Veterinary Faculty Ankara T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huseyin Burak","family":"Disli","sequence":"additional","affiliation":[{"name":"Eville and Jones, Century House Leeds UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harun","family":"Hizlisoy","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health, Faculty of Veterinary Medicine Erciyes University Kayseri T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mukaddes","family":"Barel","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health, Faculty of Veterinary Medicine Erciyes University Kayseri T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tekin","family":"Kececi","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health, Faculty of Veterinary Medicine Erciyes University Kayseri T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4679-6548","authenticated-orcid":false,"given":"Nurhan Ertas","family":"Onmaz","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health, Faculty of Veterinary Medicine Erciyes University Kayseri T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,3]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.2.619-621.2006"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.32707\/ERCIVET.1389025"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/S41598-019-54794-8"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.JKSUS.2018.07.018"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2009.04.006"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-83578-2"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/S13104-020-05339-0\/TABLES\/1"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-73.12.2225"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/ANTIBIOTICS11121755"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/TOXINS9080241"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/S00248-023-02236-4"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.IJMM.2016.11.007"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1099\/JMM.0.000540"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.MIMET.2019.105733"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.MICPATH.2018.08.031"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/FMICB.2024.1361217\/BIBTEX"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/FMICB.2019.02912"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-026-49184-w"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.3906\/SAG-1712-207"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-025-17476-2"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/S13620-021-00200-7"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/S43555-025-00067-7"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/ANTIBIOTICS4040521"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.JGAR.2016.02.006"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.IJFOODMICRO.2010.06.002"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.HELIYON.2019.E01415"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/ANTIBIOTICS12101520"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.5812\/GCT.82372"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.7508\/IBJ.2016.03.007"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.3389\/FMICB.2020.00663\/BIBTEX"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1089\/MDR.2018.0033"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/J.1574-695X.2007.00262.X"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02045-12;"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01866-06"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.12.5442-5448.2003"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/DISEASES11040160\/S1"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.5812\/JJM.98852"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/OFID\/OFAE261"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.16718\/J.1009-7708.2021.04.001"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.26.11.2465-2466.1988"},{"key":"e_1_2_12_42_1","unstructured":"ISO 6888\u20101.2021. ISO 6888\u20101:2021 \u2010 Microbiology of the Food Chain\u2014Horizontal Method for the Enumeration of Coagulase\u2010Positive Staphylococci (Staphylococcus aureus and Other Species)\u2014Part 1: Method Using Baird\u2010Parker Agar Medium.https:\/\/www.iso.org\/standard\/76672.html."},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.7717\/PEERJ.11195\/SUPP-1"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.14202\/VETWORLD.2023.46-58"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.DIAGMICROBIO.2012.03.012"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.29252\/IBJ.24.6.381"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2017.22.44.16-00696"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.5578\/mb.6589"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.2.792-799.2004\/FORMAT\/EPUB"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.FOODCONT.2018.08.030"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.IJFOODMICRO.2022.109825"},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S192611"},{"key":"e_1_2_12_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.MICPATH.2021.104915"},{"key":"e_1_2_12_54_1","doi-asserted-by":"publisher","DOI":"10.1002\/JCLA.22456"},{"key":"e_1_2_12_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.JGAR.2013.08.003"},{"key":"e_1_2_12_56_1","doi-asserted-by":"publisher","DOI":"10.1111\/J.1469-0691.2011.03570.X"},{"key":"e_1_2_12_57_1","doi-asserted-by":"publisher","DOI":"10.3389\/FCIMB.2015.00001\/FULL"},{"key":"e_1_2_12_58_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.3.1032-1035.2000"},{"key":"e_1_2_12_59_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-7-98"},{"key":"e_1_2_12_60_1","doi-asserted-by":"publisher","DOI":"10.1128\/SPECTRUM.02189-22"},{"key":"e_1_2_12_61_1","doi-asserted-by":"publisher","DOI":"10.1371\/JOURNAL.PONE.0264762"},{"key":"e_1_2_12_62_1","doi-asserted-by":"publisher","DOI":"10.1186\/S12866-020-01881-W"},{"key":"e_1_2_12_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2023.10.023"},{"key":"e_1_2_12_64_1","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12452"},{"key":"e_1_2_12_65_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/6035987"},{"key":"e_1_2_12_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.IJANTIMICAG.2023.106932"},{"key":"e_1_2_12_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.FM.2019.103249"},{"key":"e_1_2_12_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.IJFOODMICRO.2018.11.025"},{"key":"e_1_2_12_69_1","doi-asserted-by":"publisher","DOI":"10.3389\/FMICB.2025.1564943\/TEXT"},{"key":"e_1_2_12_70_1","doi-asserted-by":"publisher","DOI":"10.3390\/TOXINS14020097"},{"key":"e_1_2_12_71_1","doi-asserted-by":"publisher","DOI":"10.1371\/JOURNAL.PONE.0005082"},{"key":"e_1_2_12_72_1","doi-asserted-by":"publisher","DOI":"10.1186\/S12866-023-03065-8"},{"key":"e_1_2_12_73_1","doi-asserted-by":"publisher","DOI":"10.3390\/ANTIBIOTICS9030122"},{"key":"e_1_2_12_74_1","doi-asserted-by":"publisher","DOI":"10.1099\/MIC.0.001245\/CITE\/REFWORKS"},{"key":"e_1_2_12_75_1","doi-asserted-by":"publisher","DOI":"10.1186\/S40104-024-01007-6"},{"key":"e_1_2_12_76_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.CHROMA.2012.03.022"},{"key":"e_1_2_12_77_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(00)00122-6"},{"key":"e_1_2_12_78_1","doi-asserted-by":"publisher","DOI":"10.3390\/MICROORGANISMS11010054"},{"key":"e_1_2_12_79_1","doi-asserted-by":"publisher","DOI":"10.1007\/S42770-024-01270-9\/TABLES\/6"},{"key":"e_1_2_12_80_1","doi-asserted-by":"publisher","DOI":"10.1002\/FSN3.70791;WGROUP:STRING:PUBLICATION"},{"key":"e_1_2_12_81_1","doi-asserted-by":"publisher","DOI":"10.24099\/VET.ARHIV.1315"},{"key":"e_1_2_12_82_1","doi-asserted-by":"publisher","DOI":"10.1038\/S41579-018-0147-4"},{"key":"e_1_2_12_83_1","doi-asserted-by":"publisher","DOI":"10.1128\/MBIO.01375-16"},{"issue":"2","key":"e_1_2_12_84_1","first-page":"217","article-title":"Inducible Clindamycin Resistance in Methicillin\u2010Susceptible and Methicillin\u2010Resistant Staphylococcus aureus of Inpatient, Outpatient and Healthy Carriers in Bosnia and Herzegovina","volume":"10","author":"Uzunovi\u0107 S.","year":"2013","journal-title":"Medicinski glasnik: official publication of the Medical Association of Zenica\u2010Doboj Canton, Bosnia and Herzegovina"},{"key":"e_1_2_12_85_1","doi-asserted-by":"publisher","DOI":"10.3390\/MICROORGANISMS11040841"},{"key":"e_1_2_12_86_1","doi-asserted-by":"publisher","DOI":"10.3390\/IJMS23020948"},{"key":"e_1_2_12_87_1","volume-title":"Global Priority List of Antibiotic\u2010Resistant Bacteria to Guide Research, Discovery and Development of New Antibiotics","author":"World Health Organization","year":"2017"},{"key":"e_1_2_12_88_1","doi-asserted-by":"publisher","DOI":"10.3389\/FMICB.2019.00304"},{"key":"e_1_2_12_89_1","doi-asserted-by":"publisher","DOI":"10.3389\/FCIMB.2023.1102779"},{"key":"e_1_2_12_90_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.10.5026-5033.2005"},{"key":"e_1_2_12_91_1","doi-asserted-by":"publisher","DOI":"10.3389\/FMICB.2024.1392134\/BIBTEX"},{"key":"e_1_2_12_92_1","doi-asserted-by":"publisher","DOI":"10.3389\/FMICB.2019.01388\/TEXT"},{"key":"e_1_2_12_93_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2019.103797"}],"container-title":["MicrobiologyOpen"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mbo3.70397","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/mbo3.70397","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mbo3.70397","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T11:27:35Z","timestamp":1793100455000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mbo3.70397"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,3]]},"references-count":92,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1002\/mbo3.70397"],"URL":"https:\/\/doi.org\/10.1002\/mbo3.70397","archive":["Portico"],"relation":{},"ISSN":["2045-8827","2045-8827"],"issn-type":[{"value":"2045-8827","type":"print"},{"value":"2045-8827","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,3]]},"assertion":[{"value":"2026-02-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70397"}}