{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T15:07:06Z","timestamp":1788880026552,"version":"build-2803163510"},"reference-count":44,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003819","name":"Natural Science Foundation of Hubei Province","doi-asserted-by":"publisher","award":["2024AFC034"],"award-info":[{"award-number":["2024AFC034"]}],"id":[{"id":"10.13039\/501100003819","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Basal cell carcinoma (BCC) is the most common malignant skin tumor. Skin\u2010resident lipophilic\n                    <jats:italic>Malassezia<\/jats:italic>\n                    yeasts are associated with various cutaneous disorders, while their correlative patterns and potential biological effects in BCC tissues remain insufficiently defined. We used RT\u2010qPCR screening of archived FFPE BCC specimens and metagenomic sequencing of three paired fresh tumor and peritumoral tissues to characterize tissue\u2010associated\n                    <jats:italic>Malassezia<\/jats:italic>\n                    colonization.\n                    <jats:italic>M. globosa<\/jats:italic>\n                    was the most abundant species in FFPE samples and was also detectable in fresh tissues. In vitro functional assays (CCK\u20108, EdU) in HaCaT keratinocytes and A\u2010431 epidermoid carcinoma cells showed that 12\u2009h stimulation with optimal concentrations of\n                    <jats:italic>M. globosa<\/jats:italic>\n                    (1.2\u2009\u00d7\u200910\n                    <jats:sup>7<\/jats:sup>\n                    CFU\/mL) and\n                    <jats:italic>M. yamatoensis<\/jats:italic>\n                    (1.6\u2009\u00d7\u200910\n                    <jats:sup>7<\/jats:sup>\n                    CFU\/mL) significantly promoted epithelial cell proliferation. Transcriptome sequencing and subsequent RT\u2010qPCR validation further showed that both strains significantly upregulate pro\u2010inflammatory genes (\n                    <jats:italic>IL\u20101\u03b2<\/jats:italic>\n                    ,\n                    <jats:italic>IL\u20106<\/jats:italic>\n                    ,\n                    <jats:italic>TNF\u2010\u03b1<\/jats:italic>\n                    ) and oxidative stress\u2010related genes (\n                    <jats:italic>SOD1, SOD2)<\/jats:italic>\n                    in these cell lines. Collectively, our findings describe a correlative association between\n                    <jats:italic>Malassezia<\/jats:italic>\n                    colonization and BCC lesions and offer preliminary in vitro mechanistic clues.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.70401","type":"journal-article","created":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T14:15:02Z","timestamp":1788876902000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Malassezia May Contribute to Basal Cell Carcinoma Development via Inflammatory and Oxidative Stress Pathways"],"prefix":"10.1002","volume":"15","author":[{"given":"Xiaolei","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Dermatology, Hubei Provincial Clinical Research Center for Diagnosis and Therapeutics of Pathogenic Fungal Infection Jingzhou Hospital Affiliated to Yangtze University Jingzhou Hubei Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Hubei Provincial Clinical Research Center for Diagnosis and Therapeutics of Pathogenic Fungal Infection Jingzhou Hospital Affiliated to Yangtze University Jingzhou Hubei Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-8936-2552","authenticated-orcid":false,"given":"Tinghong","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Hubei Provincial Clinical Research Center for Diagnosis and Therapeutics of Pathogenic Fungal Infection Jingzhou Hospital Affiliated to Yangtze University Jingzhou Hubei Province 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4489-3803","authenticated-orcid":false,"given":"Yi","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Hubei Provincial Clinical Research Center for Diagnosis and Therapeutics of Pathogenic Fungal Infection Jingzhou Hospital Affiliated to Yangtze University Jingzhou Hubei Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,8]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1608-2"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2017.11.004"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2024.5640"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.12865\/chsj.48.01.16"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.5826\/dpc.1204a159"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1500542"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2023.122390"},{"issue":"1","key":"e_1_2_13_9_1","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.mehy.2011.03.020","article-title":"Could Malassezia Yeasts Be Implicated in Skin Carcinogenesis Through the Production of Aryl\u2010Hydrocarbon Receptor 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