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This study employed a comparative genomic approach to investigate the genomic characteristics, antimicrobial resistance (AMR) profiles, virulence determinants, of poultry\u2010derived MDR\n                    <jats:italic>E. coli<\/jats:italic>\n                    isolates from Bangladesh. Whole\u2010genome sequencing of three representative MDR isolates, identified with 83 globally diverse poultry, human, and environmental\n                    <jats:italic>E. coli<\/jats:italic>\n                    genomes. Pangenome analysis identified the characteristic open pangenome of\n                    <jats:italic>E. coli<\/jats:italic>\n                    , with core genes comprising only 4.6% of the combined dataset. Resistome analysis shown diverse AMR determinants, including\n                    <jats:italic>blaCTX\u2010M<\/jats:italic>\n                    ,\n                    <jats:italic>blaTEM<\/jats:italic>\n                    ,\n                    <jats:italic>sul<\/jats:italic>\n                    ,\n                    <jats:italic>tet<\/jats:italic>\n                    , and\n                    <jats:italic>qnrS1<\/jats:italic>\n                    , associated with antibiotic inactivation and efflux mechanisms. Virulence profiling revealed diverse genes involved in adhesion (\n                    <jats:italic>fim<\/jats:italic>\n                    ,\n                    <jats:italic>csg<\/jats:italic>\n                    ), iron acquisition (\n                    <jats:italic>ent<\/jats:italic>\n                    ,\n                    <jats:italic>fep<\/jats:italic>\n                    ,\n                    <jats:italic>chu<\/jats:italic>\n                    ), motility, and secretion systems, with core virulence genes exhibiting &gt;\u200990% sequence identity, whereas accessory virulence genes were more variable. Plasmid analysis demonstrated heterogeneous replicon types, predominantly IncF and Col plasmids, indicating their role in horizontal gene transfer. Jaccard similarity indices revealed moderate to high genetic overlap with global strains (~0.63 for virulence genes and ~0.55 for AMR profiles), suggesting shared evolutionary backgrounds. Phylogenomic and MLST identified all Bangladeshi isolates as ST457, clustering within a globally distributed clonal complex linked to ST10 and ST131 lineages. These findings suggest that the three Bangladeshi poultry\u2010derived\n                    <jats:italic>E. coli<\/jats:italic>\n                    isolates are genetically related to globally circulating strains while harboring extensive resistance and virulence determinants, emphasizing poultry as an important reservoir of MDR pathogens and reinforcing the need for strengthened antimicrobial stewardship and genomic surveillance.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.70406","type":"journal-article","created":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T06:24:16Z","timestamp":1789367056000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative Genomic Analysis of Multidrug\u2010Resistant\n                    <i>Escherichia coli<\/i>\n                    Across Poultry\u2013Human\u2013Environmental Interfaces"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-3680-8891","authenticated-orcid":false,"given":"Md. Rokibul Hasan","family":"Shanto","sequence":"first","affiliation":[{"name":"Department of Medicine Sylhet Agricultural University Sylhet Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. S. M.","family":"Ashab Uddin","sequence":"additional","affiliation":[{"name":"Bangladesh Livestock Research Institute (BLRI) Dhaka Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-2522-5828","authenticated-orcid":false,"given":"Md. Shakil Mahmud","family":"Supto","sequence":"additional","affiliation":[{"name":"Department of Medicine Sylhet Agricultural University Sylhet Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-8438-8828","authenticated-orcid":false,"given":"Nabil Jahan","family":"Mahim","sequence":"additional","affiliation":[{"name":"Department of Medicine Sylhet Agricultural University Sylhet Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. 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