{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T11:36:54Z","timestamp":1793101014274,"version":"4.5.0"},"reference-count":46,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Gout is a common inflammatory disease closely associated with hyperuricemia, and its global prevalence is increasing, highlighting the need for safer and more effective therapeutic strategies. Recently, microbiome\u2010based therapeutics have emerged as promising alternatives, and the development of functionally validated NGP and their advanced forms, post\u2013NGP strains, is gaining increasing attention. In this study, microbiome\u2010derived bacterial strains were screened based on their in vitro uric acid (UA)\u2010reducing capacity and hypoxanthine utilization. Among the tested strains,\n                    <jats:italic>Mitsuokella jalaludinii<\/jats:italic>\n                    exhibited the most pronounced uric acid reduction and growth enhancement in response to purine substrates compared to conventional lactic acid bacteria. This strain reduced uric acid levels by approximately 30% after 48\u2009h (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001) and showed significantly increased growth in purine\u2010enriched minimal medium (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01). WGS further confirmed its strain\u2010level novelty, leading to its designation as PMC73, and revealed the presence of genetic features associated with purine metabolism and UA regulation. In a MSU\u2010induced RAW 264.7 macrophage model, PMC73 significantly reduced UA levels (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001) and modulated immune responses by increasing anti\u2010inflammatory cytokines while decreasing pro\u2010inflammatory cytokines. These effects were associated with suppression of the NLRP3\u2013caspase\u20101, along with downregulation of URAT1 and XOD, indicating coordinated regulation of uric acid production, reabsorption, and inflammatory pathways. Based on safety evaluation, including\n                    <jats:sc>d<\/jats:sc>\n                    \u2010lactate production, cytotoxicity assays and oral toxicity assessment in a mouse model, demonstrated that PMC73 exhibited no pathogenic or toxic properties. Therefore, PMC73 represents a promising microbiome\u2010based candidate with relevance to gout\u2010associated hyperuricemia, warranting further in vivo and clinical investigations.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.70410","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T14:26:25Z","timestamp":1789482385000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Post\u2013NGP\n                    <i>Mitsuokella jalaludinii<\/i>\n                    as a Therapeutic Candidate for Gout"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-8313-0886","authenticated-orcid":false,"given":"Mohammed Solayman","family":"Hossain","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, School of Medicine Soonchunhyang University Cheonan Chungnam Republic of Korea"},{"name":"K\u2010Microbiome Institute Asan Chungnam Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sukyung","family":"Kim","sequence":"additional","affiliation":[{"name":"K\u2010Microbiome Institute Asan Chungnam Republic of Korea"},{"name":"Next\u2010Generation Microbiome Training Center Soonchunhyang University Asan Chungnam Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md Tareque","family":"Aziz","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, School of Medicine Soonchunhyang University Cheonan Chungnam Republic of Korea"},{"name":"K\u2010Microbiome Institute Asan Chungnam Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Izaz","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, School of Medicine Soonchunhyang University Cheonan Chungnam Republic of Korea"},{"name":"K\u2010Microbiome Institute Asan Chungnam Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-1785-1639","authenticated-orcid":false,"given":"Md Sarower Hossen","family":"Shuvo","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, School of Medicine Soonchunhyang University Cheonan Chungnam Republic of Korea"},{"name":"K\u2010Microbiome Institute Asan Chungnam Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-2946-1238","authenticated-orcid":false,"given":"Hyewon","family":"Yang","sequence":"additional","affiliation":[{"name":"Next\u2010Generation Microbiome Training Center Soonchunhyang University Asan Chungnam Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunjeong","family":"Jang","sequence":"additional","affiliation":[{"name":"Next\u2010Generation Microbiome Training Center Soonchunhyang University Asan Chungnam Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minseo","family":"Kim","sequence":"additional","affiliation":[{"name":"Next\u2010Generation Microbiome Training Center Soonchunhyang University Asan Chungnam Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seohyeon","family":"Jang","sequence":"additional","affiliation":[{"name":"Next\u2010Generation Microbiome Training Center Soonchunhyang University Asan Chungnam Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusol","family":"Kim","sequence":"additional","affiliation":[{"name":"Next\u2010Generation Microbiome Training Center Soonchunhyang University Asan Chungnam Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soeun","family":"Oh","sequence":"additional","affiliation":[{"name":"Next\u2010Generation Microbiome Training Center Soonchunhyang University Asan Chungnam Republic of 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