{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T06:06:34Z","timestamp":1788933994483,"version":"build-2803163510"},"reference-count":32,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2026,10]]},"abstract":"ABSTRACT<\/jats:title>\n \n Livestock wound infections are a major challenge in veterinary medicine due to the increasing prevalence of bacterial pathogens and antimicrobial resistance. This study evaluated the phytochemical composition and antibacterial activity of selected medicinal plants traditionally used for the treatment of livestock wound infections. A total of 250 livestock wound samples were processed using standard bacteriological methods, resulting in the identification of 216 bacterial isolates. The predominant bacterial pathogens included\n Staphylococcus aureus<\/jats:italic>\n (19.2%),\n Escherichia coli<\/jats:italic>\n (13.6%),\n Staphylococcus epidermidis<\/jats:italic>\n (12.0%),\n Streptococcus agalactiae<\/jats:italic>\n (9.2%),\n Salmonella Typhimurium<\/jats:italic>\n (9.2%),\n Klebsiella pneumoniae<\/jats:italic>\n (8.0%),\n Enterococcus faecalis<\/jats:italic>\n (7.6%), and\n Proteus mirabilis<\/jats:italic>\n (7.6%). Methanol, ethanol, and aqueous extracts of\n Withania somnifera, Achyranthes aspera, Verbascum sinaiticum, Aloe vera<\/jats:italic>\n , and\n Capparis zeylanica<\/jats:italic>\n were evaluated for antibacterial activity using the agar well diffusion method. Phytochemical screening revealed the presence of alkaloids, flavonoids, tannins, saponins, steroids, and phenolic compounds in most plant extracts. Among the tested plants,\n Withania somnifera<\/jats:italic>\n exhibited strong antibacterial activity against\n Staphylococcus aureus<\/jats:italic>\n , with inhibition zones ranging from 21 to 24\u2009mm, while\n Achyranthes aspera<\/jats:italic>\n demonstrated notable activity against\n E. coli<\/jats:italic>\n , producing inhibition zones of 13\u201317\u2009mm. Overall, Gram\u2010positive bacteria were more susceptible to the plant extracts than Gram\u2010negative bacteria. These findings suggest that the investigated medicinal plants possess significant antibacterial potential and may serve as promising alternative therapeutic agents for the management of livestock wound infections.\n <\/jats:p>","DOI":"10.1002\/mbo3.70414","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T05:18:26Z","timestamp":1788931106000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phytochemical Composition and Antibacterial Activity of Medicinal Plants Against Bacterial Pathogens Isolated From Livestock Wound Infections"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9940-9708","authenticated-orcid":false,"given":"Safia","family":"Arbab","sequence":"first","affiliation":[{"name":"Medicine and Engineering Interdisciplinary Research Laboratory of Nursing & Materials\/Nursing Key Laboratory of Sichuan Province, West China Hospital, Sichuan University\/West China School of Nursing Sichuan University Chengdu Sichuan China"},{"name":"Lanzhou Institute of Husbandry and Pharmaceutical Sciences Chinese Academy of Agricultural Sciences Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1052-8522","authenticated-orcid":false,"given":"Hanif","family":"Ullah","sequence":"additional","affiliation":[{"name":"Medicine and Engineering Interdisciplinary Research Laboratory of Nursing & Materials\/Nursing Key Laboratory of Sichuan Province, West China Hospital, Sichuan University\/West China School of Nursing Sichuan University Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mian Sayed","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Zoology University of Swabi Swabi Khyber Pakhtunkhwa Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khalaf F.","family":"Alsharif","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Sciences, College of Applied Medical Sciences Taif University Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuad M.","family":"Alzahrani","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Sciences, College of Applied Medical Sciences Taif University Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6688-0106","authenticated-orcid":false,"given":"Khalid J.","family":"Alzahrani","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Sciences, College of Applied Medical Sciences Taif University Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongyi","family":"Zeng","sequence":"additional","affiliation":[{"name":"General Administration Office, Jinjiang Campus, West China Hospital of Sichuan University\/West China School of Nursing Sichuan University Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Ka","sequence":"additional","affiliation":[{"name":"Medicine and Engineering Interdisciplinary Research Laboratory of Nursing & Materials\/Nursing Key Laboratory of Sichuan Province, West China Hospital, Sichuan University\/West China School of Nursing Sichuan University Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,8]]},"reference":[{"issue":"8","key":"e_1_2_11_2_1","first-page":"152","article-title":"Chemical Composition of Senecio Biafrae Leaf","volume":"2","author":"Ajiboye B.","year":"2013","journal-title":"Scientific Journal of Biological Sciences"},{"key":"e_1_2_11_3_1","first-page":"165","volume-title":"Secondary Metabolites: Secondary Metabolic Products Consisting of C and H; C, H, and O; N, S, and P Elements; and O\/N Heterocycles, Therapeutic use of Medicinal Plants and Their Extracts: Volume 2: Phytochemistry and Bioactive Compounds","author":"Alamgir A.","year":"2018"},{"issue":"8","key":"e_1_2_11_4_1","doi-asserted-by":"crossref","first-page":"480","DOI":"10.3390\/antibiotics9080480","article-title":"Plant\u2010Based Phytochemicals as Possible Alternative to Antibiotics in Combating Bacterial Drug Resistance","volume":"9","author":"AlSheikh H. 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