{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T11:10:02Z","timestamp":1788952202115,"version":"build-2803163510"},"reference-count":47,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T00:00:00Z","timestamp":1788912000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T00:00:00Z","timestamp":1788912000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study provides the first metagenomic assessment of microbial diversity from the tea rhizosphere of the Kangra valley. Tea rhizosphere soil samples were collected from 4 locations (Dharamshala, Baijnath, Palampur, and Joginder Nagar) of the Kangra valley. DNA extracts of rhizosphere samples were analysed for bacterial and Archaeal diversity using amplicon sequencing (V3\u2010V4) region of the 16S rRNA gene and Fungal diversity using ITS1 and ITS2 regions. Baijnath and Palampur samples showed the highest bacterial richness, while Dharamshala and Palampur had the highest fungal richness. Proteobacteria was a dominant phylum in all the rhizosphere samples, followed by Firmicutes, Actinobacteria, Acidobacteria, and Bacteroidetes. A total of 11 fungal phyla were identified among all the locations, with abundance of Ascomycota and Basidiomycota. For the Archaea domain, uncultured archaeon and\n                    <jats:italic>Aeropyrum camini<\/jats:italic>\n                    were the most common found among all the locations. A small fraction (&lt;\u20090.5%) of\n                    <jats:italic>Bacillus<\/jats:italic>\n                    and\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    species were observed among all the locations. Alpha and beta diversity indices displayed notable differences within and between microbial diversities. Soil factors were variably associated with microbial diversity, with nitrogen positively aligned with fungal diversity, while EC and K were associated with Archaeal diversity. Soil pH and OM% showed moderate associations with bacterial diversity. These findings provided valuable and comprehensive insights into tea rhizosphere microbial ecology and could be used to better understand microbial functions and their role in plant health.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.70416","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T10:15:52Z","timestamp":1788948952000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Metagenomic Insights Into Microbial Diversity of Tea Rhizosphere of the Kangra Valley"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-4153-5658","authenticated-orcid":false,"given":"Rishu","family":"Thakur","sequence":"first","affiliation":[{"name":"CSIR\u2010Institute of Himalayan Bioresource Technology Palampur Himachal Pradesh India"},{"name":"Menzies School of Health Research Charles Darwin University Alice Springs Northern Territory Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hena","family":"Dhar","sequence":"additional","affiliation":[{"name":"CSIR\u2010Institute of Himalayan Bioresource Technology Palampur Himachal Pradesh India"},{"name":"School of Biotechnology, Faculty of Applied Sciences and Biotechnology Shoolini University Solan Himachal Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shashi","family":"Kiran","sequence":"additional","affiliation":[{"name":"CSIR\u2010Institute of Himalayan Bioresource Technology Palampur Himachal Pradesh India"},{"name":"Universit\u00e9 Paris Cit\u00e9, INSERM UMR\u2010S1451, CNRS UMR\u2010S10253, Institut Necker Enfants Malades Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arvind","family":"Gulati","sequence":"additional","affiliation":[{"name":"CSIR\u2010Institute of Himalayan Bioresource Technology Palampur Himachal Pradesh 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