{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T06:49:34Z","timestamp":1791528574496,"version":"4.3.4"},"reference-count":44,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:00:00Z","timestamp":1791417600000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001688","name":"International Centre for Genetic Engineering and Biotechnology","doi-asserted-by":"publisher","award":["CRP\/ZAF22\u201093"],"award-info":[{"award-number":["CRP\/ZAF22\u201093"]}],"id":[{"id":"10.13039\/501100001688","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The rhizosphere microbiome is extremely important for soil health and plant productivity, with fungi primarily acting as the drivers of soil ecosystem functions. This study explores the fungal diversity and predicted functional potential of the soybean (Glycine max L.) rhizosphere under different organic fertilization treatments, including cattle dung, poultry waste, untreated control, and bulk. Fungal communities were profiled using ITS rRNA gene amplicon sequencing on the Illumina NovaSeq. 6000 platform following DNA extraction from rhizosphere soil. Raw sequences were processed and analyzed using QIIME 2 version 2019.1. Significant differences in fungal richness were observed among treatments. Bulk soil and cattle dung\u2010amended soils showed the highest richness (Chao1\u2009=\u20091603.33 and 1456.64, respectively), whereas poultry waste\u2010amended soil exhibited the lowest richness (Chao1\u2009=\u2009957.93) and the lowest evenness (Pielou's index\u2009=\u20090.560), and the untreated control showed intermediate richness (Chao1\u2009=\u20091242.11). The dominant phyla include Ascomycota, Basidiomycota, and Zygomycota, with enrichment in organic matter degradation and plant symbiosis. Beta\u2010diversity analysis showed the greatest compositional divergence between cattle dung and poultry waste (ANOSIM\n                    <jats:italic>R<\/jats:italic>\n                    \u2009=\u20090.74), none of the pairwise comparisons reached conventional statistical significance (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&gt;\u20090.05), indicating ecologically relevant but statistically subtle shifts in community structure. LEfSe identified Hypocreales as a significant biomarker of cattle dung and Onygenales of poultry waste, consistent with FUNGuild predictions of enriched dung\/wood\u2010saprotrophic and keratinolytic functions, respectively. FUNGuild predictions showed increased saprotroph and symbiotroph diversity in organically amended soils. Organic fertilizers enrich beneficial fungi, enhancing ecosystem functions in sustainable soybean cultivation.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.70437","type":"journal-article","created":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T05:59:16Z","timestamp":1791525556000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Amplicon Characterization of Fungal Diversity and Functional Prediction of Glycine max L. Rhizosphere Microbiome Under Different Organic Fertilization"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-8933-7623","authenticated-orcid":false,"given":"Ijeoma Emelda","family":"Osuji","sequence":"first","affiliation":[{"name":"Soil\u2010Plant\u2010Microbe Interactions Group, Faculty of Natural and Agricultural Sciences North\u2010West University Mmabatho South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2816-9820","authenticated-orcid":false,"given":"Akinlolu Olalekan","family":"Akanmu","sequence":"additional","affiliation":[{"name":"Soil\u2010Plant\u2010Microbe Interactions Group, Faculty of Natural and Agricultural Sciences North\u2010West University Mmabatho South Africa"},{"name":"Department of Biological Sciences, College of Basic Sciences Lagos State University of Science and Technology Ikorodu Lagos Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4344-1909","authenticated-orcid":false,"given":"Olubukola Oluranti","family":"Babalola","sequence":"additional","affiliation":[{"name":"Soil\u2010Plant\u2010Microbe Interactions Group, Faculty of Natural and Agricultural Sciences North\u2010West University Mmabatho South Africa"},{"name":"Department of Life Sciences Imperial College London Ascot Berkshire UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"crossref","DOI":"10.3390\/ijms23169422","article-title":"Functional Diversity of Microbial Communities in the Soybean (Glycine max L.) 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O.","year":"2023","journal-title":"Horticulturae"},{"key":"e_1_2_10_5_1","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1186\/s12863-025-01351-0","article-title":"Dataset of 16S rRNA and ITS Gene Amplicon Sequencing of Celery and Parsley Rhizosphere Soils","volume":"26","author":"Babalola O. O.","year":"2025","journal-title":"BMC Genomic Data"},{"key":"e_1_2_10_6_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12863-025-01333-2","article-title":"Amplicon\u2010Based Metagenomic Survey of Microbes Associated With the Organic and Inorganic Rhizosphere Soil of Glycine max L","volume":"26","author":"Babalola O. 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