{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T08:51:31Z","timestamp":1791967891558,"version":"4.3.4"},"reference-count":65,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Early\u2010life gut microbiota plays a critical role in livestock health and productivity. Probiotic interventions, particularly those employing bacteriocin\u2010producing lactic acid bacteria (LAB), may contribute to modulation of microbial development during this critical period. This study evaluated\n                    <jats:italic>Lactiplantibacillus pentosus<\/jats:italic>\n                    AA86 (formerly\n                    <jats:italic>Lactobacillus pentosus<\/jats:italic>\n                    ) and\n                    <jats:italic>Pediococcus pentosaceus<\/jats:italic>\n                    AA94 for genomic safety, bacteriocin potential, and their effects on calf gut microbiota. Whole\u2010genome sequencing assessed antimicrobial resistance genes, virulence determinants, plasmid\u2010associated elements, and bacteriocin\u2010encoding genes. Both strains lacked detectable antimicrobial resistance genes, virulence determinants, and plasmid\u2010related elements, while harboring pediocin\u2010encoding genes consistent with potential antimicrobial functionality. In a 30\u2010day feeding trial, eight calves (50\u2009\u00b1\u200910\u2009kg) were assigned to a control group (sterile skimmed milk,\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20092) or a probiotic group (milk supplemented with both strains at ~8.6\u2009\u00d7\u200910\n                    <jats:sup>10<\/jats:sup>\n                    CFU\/day,\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20096). Gut microbiota was evaluated at baseline, mid\u2010, and post\u2010intervention using 16S rRNA gene (V3\/V4) sequencing and QIIME2. Alpha diversity and overall community structure did not differ significantly between groups. However, supplementation was associated with selective temporal compositional shifts, particularly in Firmicutes and Bacteroidetes. At the genus level,\n                    <jats:italic>Lactobacillus<\/jats:italic>\n                    showed significant temporal changes within treatment group, although the treatment\u2010by\u2010time interaction was not significant.\n                    <jats:italic>L. pentosus<\/jats:italic>\n                    AA86 and\n                    <jats:italic>P. pentosaceus<\/jats:italic>\n                    AA94 exhibited favorable genomic safety profiles and bacteriocin\u2010associated antimicrobial potential. Their supplementation was associated with selective temporal modulation of the developing calf gut microbiota without substantial alteration of overall community structure. These findings provide a foundation for larger and longer\u2010term studies incorporating strain\u2010resolved, functional, metabolomic, and host health analyses.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.70438","type":"journal-article","created":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T08:22:47Z","timestamp":1791966167000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Modulation of Early\u2010Life Calf Gut Microbiota by Bacteriocin\u2010Producing\n                    <i>Lactiplantibacillus pentosus<\/i>\n                    AA86 and\n                    <i>Pediococcus pentosaceus<\/i>\n                    AA94"],"prefix":"10.1002","volume":"15","author":[{"given":"Adewale A.","family":"Adetoye","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmacy Lead City University Ibadan  Oyo State Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1405-2365","authenticated-orcid":false,"given":"Ayorinde O.","family":"Afolayan","sequence":"additional","affiliation":[{"name":"Leibniz Institute DSMZ \u2013 German Collection of Microorganisms and Cell Cultures Braunschweig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isaac O.","family":"Olatoye","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine University of Ibadan  Oyo State Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6163-286X","authenticated-orcid":false,"given":"Adedayo O.","family":"Faneye","sequence":"additional","affiliation":[{"name":"Department of Virology, College of Medicine University of Ibadan  Oyo State Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-4333-6174","authenticated-orcid":false,"given":"Funmilola A.","family":"Ayeni","sequence":"additional","affiliation":[{"name":"Department of Environmental and Occupational Health, School of Public Health Indiana University, Bloomington Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,14]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.4314\/ahs.v15i3.24"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-018-1248-y"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.csbj.2021.01.035"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/msystems.00191-16"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.851140"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aninu.2021.03.004"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1487664"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2025.1672780"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2276"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.7287\/peerj.preprints.27295v2"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114516001045"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01840-06"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.csbj.2025.10.050"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11259-025-10927-1"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0204253"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1101\/672295"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12916-025-04602-0"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.772863"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.afres.2025.101097"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-70549-6"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-72296-0"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvfa.2019.08.002"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-74677-7"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods6070053"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/applmicrobiol5040149"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.709959"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aninu.2023.03.003"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms10091700"},{"key":"e_1_2_10_30_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13073-016-0300-5","article-title":"Alterations in Fecal Microbiota Composition by Probiotic Supplementation in Healthy Adults: A Systematic Review of Randomized Con\u2010Trolled Trials","volume":"8","author":"Kristensen N. B.","year":"2016","journal-title":"Genome Medicine"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/vms3.70742"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2676"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms13081810"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1249628"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics10050578"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.12.8228-8235.2005"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu12051474"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2015.00036"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-16786-z"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2011.139"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0061217"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.00582"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2010.05.001"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2016.02.002"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10123-023-00462-x"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2025.1605215"},{"issue":"2017","key":"e_1_2_10_47_1","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1186\/s40168-017-0268-4","article-title":"A Critical Assessment of the \u201cSterile Womb\u201d and \u201cIn Utero Colonization\u201d Hypotheses: Implications for Research on the Pioneer Infant Microbiome","volume":"5","author":"Perez\u2010Mu\u00f1oz M. E.","year":"2017","journal-title":"Microbiome"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0009490"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.5772\/intechopen.1009998"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-024-03948-y"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/asj.13872"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1240267"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-40703-7"},{"key":"e_1_2_10_54_1","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1186\/s12916-016-0629-z","article-title":"Probiotics and Microbiota Composition","volume":"14","author":"Sanders M. E.","year":"2016","journal-title":"BMC Medicine"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.02.044"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lwt.2020.110550"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods11193145"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1264\/jsme2.ME14176"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1503141112"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky383"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2025.11.009"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40104-022-00806-z"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms10071391"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms12010012"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani11123431"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxics13050348"}],"container-title":["MicrobiologyOpen"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mbo3.70438","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/mbo3.70438","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mbo3.70438","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T08:22:49Z","timestamp":1791966169000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mbo3.70438"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":65,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1002\/mbo3.70438"],"URL":"https:\/\/doi.org\/10.1002\/mbo3.70438","archive":["Portico"],"relation":{},"ISSN":["2045-8827","2045-8827"],"issn-type":[{"value":"2045-8827","type":"print"},{"value":"2045-8827","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"2026-04-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70438"}}