{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T18:28:46Z","timestamp":1782930526054,"version":"3.54.5"},"reference-count":20,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,8,29]],"date-time":"2018-08-29T00:00:00Z","timestamp":1535500800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2018,8,29]],"date-time":"2018-08-29T00:00:00Z","timestamp":1535500800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100008370","name":"National Pork Board","doi-asserted-by":"publisher","award":["16\u2010192"],"award-info":[{"award-number":["16\u2010192"]}],"id":[{"id":"10.13039\/100008370","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Salmonella<\/jats:italic>\n                    is a pathogen of public health concern. Each year,\n                    <jats:italic>Salmonella<\/jats:italic>\n                    infections cost to the food industry approximately $2.3 billion and 33% of the reported cases are associated with beef, poultry, or pork. Pathogen presence in feed mills can represent one of the many potential routes for entry and transmission into the food production chain. Nevertheless, little is known about\n                    <jats:italic>Salmonella<\/jats:italic>\n                    incidence and association with these types of environments. The objective of this study was to investigate\n                    <jats:italic>Salmonella<\/jats:italic>\n                    presence in different feed mills across the United States. Eleven facilities were selected in eight states and 12 sites were sampled within each feed mill. Samples were analyzed following the FSIS guidelines for isolation and identification of\n                    <jats:italic>Salmonella<\/jats:italic>\n                    . Positive isolates were further investigated by a PCR analysis targeting the\n                    <jats:italic>invA<\/jats:italic>\n                    gene to differentiate for\n                    <jats:italic>Salmonella enterica<\/jats:italic>\n                    . The total number of environmental samples collected was 237: 66% resulted culture positive and 13.1% were PCR positive. All sampled feed mills had at least one culture positive site and following production flow the number of positive samples decreased from ingredient receiving to final product. These preliminary results demonstrate the presence of\n                    <jats:italic>Salmonella<\/jats:italic>\n                    in selected United States feed mills and suggest their potential role as vehicle for pathogen transmission and spread into the food production chain.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.711","type":"journal-article","created":{"date-parts":[[2018,8,30]],"date-time":"2018-08-30T03:30:30Z","timestamp":1535599830000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Evaluation of\n                    <i>Salmonella<\/i>\n                    presence in selected United States feed mills"],"prefix":"10.1002","volume":"8","author":[{"given":"Gabriela","family":"Magossi","sequence":"first","affiliation":[{"name":"Food Science Institute Kansas State University  Manhattan Kansas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Cernicchiaro","sequence":"additional","affiliation":[{"name":"Diagnostic Medicine\/Pathobiology College of Veterinary Medicine Kansas State University  Manhattan Kansas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steve","family":"Dritz","sequence":"additional","affiliation":[{"name":"Diagnostic Medicine\/Pathobiology College of Veterinary Medicine Kansas State University  Manhattan Kansas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terry","family":"Houser","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences and Industry Kansas State University  Manhattan Kansas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason","family":"Woodworth","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences and Industry Kansas State University  Manhattan Kansas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cassandra","family":"Jones","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences and Industry Kansas State University  Manhattan Kansas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3329-3172","authenticated-orcid":false,"given":"Valentina","family":"Trinetta","sequence":"additional","affiliation":[{"name":"Food Science Institute Kansas State University  Manhattan Kansas"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,8,29]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2009.01.009"},{"key":"e_1_2_8_3_1","unstructured":"Bacterial Analytical Manual. (2011).Chapter 5: Salmonella.https:\/\/www.fda.gov\/Food\/FoodScienceResearch\/LaboratoryMethods\/ucm070149.htm"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/1541-4337.12001"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2018.03.019"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2015.07.002"},{"key":"e_1_2_8_7_1","unstructured":"Centers for Disease Control and Prevention (CDC). (2014).National Enteric disease surveillance: Salmonella surveillance overview.https:\/\/www.cdc.gov\/nationalsurveillance\/salmonella-surveillance.html"},{"key":"e_1_2_8_8_1","doi-asserted-by":"crossref","first-page":"154","DOI":"10.54846\/jshap\/952","article-title":"Feed mill biosecurity plans: A systematic approach to prevent biological pathogens in swine feed","volume":"24","author":"Cochrane R. A.","year":"2015","journal-title":"Journal of Swine Health and Production"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1086\/342885"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2004.1.202"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.5367\/000000001101293427"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02991-09"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3382\/japr.2010-00281"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/ps\/83.3.384"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2011.1083"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01169-10"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/1751-0147-47-13"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-73.10.1919"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2017.03.017"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2011.04.041"},{"key":"e_1_2_8_21_1","volume-title":"Food Safety and Inspection Service. Isolation and identification of Salmonella from meat, poultry, pasteurized egg, and catfish products and carcass and environmental sponges","author":"United States Department of Agriculture Food Safety and Inspection Service (USDA\/FSIS)","year":"2014"}],"container-title":["MicrobiologyOpen"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmbo3.711","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mbo3.711","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/mbo3.711","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mbo3.711","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T08:55:52Z","timestamp":1772960152000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mbo3.711"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8,29]]},"references-count":20,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2019,5]]}},"alternative-id":["10.1002\/mbo3.711"],"URL":"https:\/\/doi.org\/10.1002\/mbo3.711","archive":["Portico"],"relation":{},"ISSN":["2045-8827","2045-8827"],"issn-type":[{"value":"2045-8827","type":"print"},{"value":"2045-8827","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,8,29]]},"assertion":[{"value":"2018-04-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-07-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-08-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00711"}}