{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T06:56:29Z","timestamp":1792133789489,"version":"4.4.0"},"reference-count":63,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,9,21]],"date-time":"2018-09-21T00:00:00Z","timestamp":1537488000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2018,9,21]],"date-time":"2018-09-21T00:00:00Z","timestamp":1537488000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"name":"NSF NEON","award":["1010-0264-000"],"award-info":[{"award-number":["1010-0264-000"]}]},{"name":"University of North Texas Health Science Center"},{"name":"State of Texas"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Ixodes scapularis<\/jats:italic>\n                    , the blacklegged deer tick, is the principal vector of Lyme disease in North America. Environmental factors are known to influence regional and seasonal incidence of Lyme disease and possibly the endemicity of the disease to the northeastern and upper mid\u2010western regions of the United States. With a goal to understand the impact of environmental temperature on microbial communities within the tick, we investigated the bacterial microbiome of colony\u2010reared\n                    <jats:italic>I.\u00a0scapularis<\/jats:italic>\n                    ticks statically incubated at different temperatures (4, 20, 30, and 37\u00b0C) at a constant humidity in a controlled laboratory setting by comparison of sequenced amplicons of the bacterial 16S V4\n                    <jats:styled-content style=\"fixed-case\">rRNA<\/jats:styled-content>\n                    gene to that of the untreated baseline controls. The microbiomes of colony\u2010reared\n                    <jats:italic>I.\u00a0scapularis<\/jats:italic>\n                    males were distinct than that of females, which were entirely dominated by\n                    <jats:italic>Rickettsia<\/jats:italic>\n                    . In silico removal of\n                    <jats:italic>Rickettsia<\/jats:italic>\n                    sequences from female data revealed the underlying bacterial community, which is consistent in complexity with those seen among male ticks. The bacterial community composition of these ticks changes upon incubation at 30\u00b0C for a week and 37\u00b0C for more than 5\u00a0days. Moreover, the male ticks incubated at 30 and 37\u00b0C exhibited significantly different bacterial diversity compared to the initial baseline microbiome, and the change in bacterial diversity was dependent upon duration of exposure.\n                    <jats:italic>Rickettsia<\/jats:italic>\n                    \u2010free data revealed a significantly different bacterial diversity in female ticks incubated at 37\u00b0C compared to that of 4 and 20\u00b0C treatments. These results provide experimental evidence that environmental temperature can impact the tick bacterial microbiome in a laboratory setting.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.719","type":"journal-article","created":{"date-parts":[[2018,9,21]],"date-time":"2018-09-21T18:54:31Z","timestamp":1537556071000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":81,"title":["Effects of temperature on bacterial microbiome composition in\n                    <i>Ixodes scapularis<\/i>\n                    ticks"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4534-1349","authenticated-orcid":false,"given":"Santosh","family":"Thapa","sequence":"first","affiliation":[{"name":"Tick Borne Disease Research Laboratory Department of Microbiology, Immunology and Genetics University of North Texas Health Science Center  Fort Worth Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Tick Borne Disease Research Laboratory Department of Microbiology, Immunology and Genetics University of North Texas Health Science Center  Fort Worth Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2293-8078","authenticated-orcid":false,"given":"Michael S.","family":"Allen","sequence":"additional","affiliation":[{"name":"Tick Borne Disease Research Laboratory Department of Microbiology, Immunology and Genetics University of North Texas Health Science Center  Fort Worth Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,9,21]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1890\/12-2010.1"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid0402.980205"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1467-9868.2010.00746.x"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.21.10034"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2017.00236"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7043737"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0025604"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.mm6436a8"},{"key":"e_1_2_10_10_1","volume-title":"Tickborne diseases of the United States: A reference manual for health care providers","author":"CDC","year":"2015"},{"key":"e_1_2_10_11_1","unstructured":"CDC(Centers for Disease Control and Prevention) (2017a).Geographic distribution of ticks that bite humans: blacklegged tick (Ixodes scapularis)[December 15 2017]. 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