{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T04:23:17Z","timestamp":1790310197048,"version":"4.1.0"},"reference-count":26,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,10,8]],"date-time":"2018-10-08T00:00:00Z","timestamp":1538956800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2018,10,8]],"date-time":"2018-10-08T00:00:00Z","timestamp":1538956800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2015\/10332\u20106"],"award-info":[{"award-number":["2015\/10332\u20106"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The aims of this study were to determine the antimicrobial susceptibility profile and genetic diversity of\n                    <jats:italic>Staphylococcus<\/jats:italic>\n                    spp. isolated from dairy cows in Minas Gerais, Brazil, and to assess the relationship among the isolates\u2019 susceptibility profiles and pulsed\u2010field gel electrophoresis (\n                    <jats:styled-content style=\"fixed-case\">PFGE<\/jats:styled-content>\n                    ) genotypes. Seventy\u2010nine isolates were used, including\n                    <jats:italic>S.\u00a0aureus<\/jats:italic>\n                    (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a071) and coagulase\u2010negative staphylococci (Co\n                    <jats:styled-content style=\"fixed-case\">NS<\/jats:styled-content>\n                    ) (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a08). Susceptibility to 12 antimicrobial agents was performed. All\n                    <jats:italic>Staphylococcus<\/jats:italic>\n                    spp. were subjected to\n                    <jats:styled-content style=\"fixed-case\">PFGE<\/jats:styled-content>\n                    .\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    and Co\n                    <jats:styled-content style=\"fixed-case\">NS<\/jats:styled-content>\n                    isolates exhibited full susceptibility only to cephalothin. The greatest percentages of resistance among\n                    <jats:italic>Staphylococcus<\/jats:italic>\n                    spp. were observed to penicillins, folate pathway inhibitors, and tetracyclines. Twelve\n                    <jats:italic>S.\u00a0aureus<\/jats:italic>\n                    and four Co\n                    <jats:styled-content style=\"fixed-case\">NS<\/jats:styled-content>\n                    were classified as multidrug resistance strains. Percentage of\n                    <jats:styled-content style=\"fixed-case\">MRSA<\/jats:styled-content>\n                    was also higher among Co\n                    <jats:styled-content style=\"fixed-case\">NS<\/jats:styled-content>\n                    (75%), compared to\n                    <jats:italic>S.\u00a0aureus<\/jats:italic>\n                    isolates (2.81%). Adopting 100% of similarity, 34 different genotypes were identified. Association of minimum\u2010spanning tree (\n                    <jats:styled-content style=\"fixed-case\">MST<\/jats:styled-content>\n                    ) analysis with data from municipalities, herds, methicillin\u2010resistant\n                    <jats:italic>S.\u00a0aureus<\/jats:italic>\n                    (\n                    <jats:styled-content style=\"fixed-case\">MRSA<\/jats:styled-content>\n                    ), and resistance patterns for all isolates did not show any clustering. However, a clustering pattern of bacterial species was observed. Results from this study indicate a high frequency of antimicrobial resistance, especially among Co\n                    <jats:styled-content style=\"fixed-case\">NS<\/jats:styled-content>\n                    , and a high genetic diversity among\n                    <jats:italic>Staphylococcus<\/jats:italic>\n                    spp. isolated from dairy cows with mastitis in Minas Gerais, Brazil.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.736","type":"journal-article","created":{"date-parts":[[2018,10,9]],"date-time":"2018-10-09T01:04:44Z","timestamp":1539047084000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Genetic diversity and antimicrobial resistance in\n                    <i>Staphylococcus aureus<\/i>\n                    and coagulase\u2010negative\n                    <i>Staphylococcus<\/i>\n                    isolates from bovine mastitis in Minas Gerais, Brazil"],"prefix":"10.1002","volume":"8","author":[{"given":"Elaine M. S.","family":"Dorneles","sequence":"first","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Universidade Federal de Lavras Lavras Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana D. A. M.","family":"Fonseca","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Preventiva e Sa\u00fade Animal Faculdade de Medicina Veterin\u00e1ria e Zootecnia Universidade de S\u00e3o Paulo S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana A. P.","family":"Abreu","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Preventiva e Sa\u00fade Animal Faculdade de Medicina Veterin\u00e1ria e Zootecnia Universidade de S\u00e3o Paulo S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrey P.","family":"Lage","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Preventiva Escola de Veterin\u00e1ria Universidade Federal de Minas Gerais Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria A. V. P.","family":"Brito","sequence":"additional","affiliation":[{"name":"N\u00facleo de Sa\u00fade Animal e Qualidade do Leite Embrapa Gado de Leite Juiz de Fora Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carine R.","family":"Pereira","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Universidade Federal de Lavras Lavras Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Humberto M.","family":"Brand\u00e3o","sequence":"additional","affiliation":[{"name":"N\u00facleo de Sa\u00fade Animal e Qualidade do Leite Embrapa Gado de Leite Juiz de Fora Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro S.","family":"Guimar\u00e3es","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Universidade Federal de Lavras Lavras Minas Gerais Brazil"},{"name":"N\u00facleo de Sa\u00fade Animal e Qualidade do Leite Embrapa Gado de Leite Juiz de Fora Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6444-9788","authenticated-orcid":false,"given":"Marcos B.","family":"Heinemann","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Preventiva e Sa\u00fade Animal Faculdade de Medicina Veterin\u00e1ria e Zootecnia Universidade de S\u00e3o Paulo S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,10,8]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-736X2016000700001"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2016.11.002"},{"key":"e_1_2_9_4_1","unstructured":"Alves C. 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