{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T03:24:58Z","timestamp":1790652298331,"version":"4.1.0"},"reference-count":53,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,10,24]],"date-time":"2018-10-24T00:00:00Z","timestamp":1540339200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2018,10,24]],"date-time":"2018-10-24T00:00:00Z","timestamp":1540339200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31501980"],"award-info":[{"award-number":["31501980"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31572436"],"award-info":[{"award-number":["31572436"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The objective of this study was to investigate the impact of a high\u2010grain (HG) diet on the microbial fermentation, the composition of the mucosa\u2010associated bacterial microbiota, and the gene expression of tight junction proteins in the small intestine of goats. In the present study, we randomly assigned 10 male goats to either a hay diet (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a05) or a HG diet (56.5% grain;\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a05) and then examined changes in the bacterial community using Illumina MiSeq sequencing and the expression of tight junction proteins using qRT\u2010PCR in the mucosa of the small intestine. The results showed that HG diet decreased the luminal pH (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0=\u00a00.005) and increased the lipopolysaccharide content (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.001) in the digesta of the ileum, and it increased the concentration of total volatile fatty acids in the digesta of the jejunum (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0=\u00a00.015) and ileum (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0=\u00a00.007) compared with the hay diet. MiSeq sequencing results indicated that the HG diet increased (FDR\u00a0=\u00a00.007\u20130.028) the percentage of the genera\n                    <jats:italic>Stenotrophomonas<\/jats:italic>\n                    ,\n                    <jats:italic>Moraxella<\/jats:italic>\n                    ,\n                    <jats:italic>Lactobacillus<\/jats:italic>\n                    , and\n                    <jats:italic>Prevotella<\/jats:italic>\n                    in jejunal mucosa but decreased (FDR\u00a0=\u00a00.016) the abundance of\n                    <jats:italic>Christensenellaceae R7<\/jats:italic>\n                    group in the ileal mucosa compared with the hay diet. Furthermore, the HG diet caused downregulation of the mRNA expression of claudin\u20104, occludin, and ZO\u20101 in jejunal and ileal mucosa (\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0&lt;\u00a00.05). Collectively, our data suggested that the HG diet induced changes in the relative abundance of some mucosa\u2010associated bacteria, in addition to downregulation of the mRNA expression of tight junction proteins in the small intestine. These findings provide new insights into the adaptation response of the small intestine to HG feeding in ruminants.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.745","type":"journal-article","created":{"date-parts":[[2018,10,25]],"date-time":"2018-10-25T00:08:41Z","timestamp":1540426121000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Impact of high\u2010grain diet feeding on mucosa\u2010associated bacterial community and gene expression of tight junction proteins in the small intestine of goats"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1028-5586","authenticated-orcid":false,"given":"Junhua","family":"Liu","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology Nanjing Agricultural University Nanjing Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunxu","family":"Xue","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology Nanjing Agricultural University Nanjing Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daming","family":"Sun","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology Nanjing Agricultural University Nanjing Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiyun","family":"Zhu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology Nanjing Agricultural University Nanjing Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengyong","family":"Mao","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology Nanjing Agricultural University Nanjing Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,10,24]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1086\/502197"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfoodeng.2005.12.053"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-47-2-284"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)51379-X"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2005-747"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.2527\/1999.7771889x"},{"key":"e_1_2_8_8_1","first-page":"1","article-title":"Isolation and identification of sodium fluoroacetate degrading bacteria from caprine rumen in Brazil","volume":"2012","author":"Camboim E. 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