{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T12:03:09Z","timestamp":1786622589675,"version":"build-2736575974"},"reference-count":41,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2019,3,19]],"date-time":"2019-03-19T00:00:00Z","timestamp":1552953600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Elizabethkingia anophelis<\/jats:italic> 12012\u20102 PRCM was isolated from a patient with multiple organ dysfunction syndrome and lower respiratory tract infection in China. Minimum inhibitory concentration (MIC) analysis demonstrated that it was resistant to 20 antibiotics including trimethoprim\/sulfamethoxazole and ciprofloxacin, which were effective for the elimination of other <jats:italic>Elizabethkingia<\/jats:italic> infections. To investigate multidrug resistance and pathogenicity mechanisms, we analyzed genome features of 12012\u20102 PRCM and compared them to the other <jats:italic>Elizabethkingia<\/jats:italic> species. The draft genome size was 4.02\u00a0Mb with a GC content of 32%, comparable to that of other <jats:italic>E. anophelis<\/jats:italic> strains. Phylogenetic analysis showed that <jats:italic>E. anophelis<\/jats:italic> 12012\u20102 PRCM formed a sister group with <jats:italic>E. anophelis<\/jats:italic> 502, distinct from clades formed by other clinical and environmental <jats:italic>E. anophelis<\/jats:italic> isolates. <jats:italic>E. anophelis<\/jats:italic> 12012\u20102 PRCM contained multiple copies of <jats:italic>\u03b2<\/jats:italic>\u2010lactamase genes as well as genes predicted to function in antimicrobial efflux. It also contained 92 genes that were potentially involved in virulence, disease, and defense, and were associated with resistance and pathogenicity. Comparative genomic analysis showed high homology among three clinical and two environmental <jats:italic>E. anophelis<\/jats:italic> strains having a variety of similar antibiotic resistance and virulence factor genes, and similar genomic structure. Applications of this analysis will contribute to understanding the antibiotic resistance and pathogenic mechanisms of <jats:italic>E. anophelis <\/jats:italic>infections, which will assist in the management of infections as it increases in prevalence.<\/jats:p>","DOI":"10.1002\/mbo3.804","type":"journal-article","created":{"date-parts":[[2019,3,20]],"date-time":"2019-03-20T04:51:14Z","timestamp":1553057474000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["The antibiotic resistance and pathogenicity of a multidrug\u2010resistant <i>Elizabethkingia anophelis<\/i> isolate"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8093-0384","authenticated-orcid":false,"given":"Mingxi","family":"Wang","sequence":"first","affiliation":[{"name":"Yun Leung Laboratory for Molecular Diagnostics, School of Medicine Huaqiao University Xiamen, Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongzhi","family":"Gao","sequence":"additional","affiliation":[{"name":"Clinical Center for Molecular Diagnosis and Therapy Fujian Medical University 2nd Affiliated Hospital Quanzhou, Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nanfei","family":"Lin","sequence":"additional","affiliation":[{"name":"Clinical Center for Molecular Diagnosis and Therapy Fujian Medical University 2nd Affiliated Hospital Quanzhou, Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaping","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine Fujian Medical University 2nd Affiliated Hospital Quanzhou, Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Huang","sequence":"additional","affiliation":[{"name":"Quanzhou Medical College Quanzhou, Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward D.","family":"Walker","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics Michigan State University East Lansing Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Desong","family":"Ming","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Quanzhou First Hospital Affiliated to Fujian Medical University Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shicheng","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics Michigan State University East Lansing Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8687-6905","authenticated-orcid":false,"given":"Shaohua","family":"Hu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases The First Affiliated Hospital of Medical College, Zhejiang University Hangzhou, Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,3,19]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2008.86"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-9-75"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plasmid.2017.04.002"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msu088"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr846"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-8-209"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw255"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep30379"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr989"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv1239"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0011147"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)60318-9"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M701876200"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01924-15"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01424-15"},{"issue":"1","key":"e_1_2_10_17_1","first-page":"274","article-title":"Relative prevalence and antimicrobial susceptibility of clinical Isolates of Elizabethkingia species based on 16S rRNA","volume":"55","author":"Han M. 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