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No specific treatment is currently available and the preventative vaccine is only partially protective. To develop a potential drug target for dengue fever, we need to understand its biology and pathogenesis thoroughly. <jats:italic>N<\/jats:italic>\u2010myristoyltransferase (NMT) is an N\u2010terminal protein lipidation enzyme that catalyzes the covalent cotranslational attachment of fatty acids to the amino\u2010terminal glycine residue of a number of proteins, leading to the modulation of various signaling molecules. In this study, we investigated the interaction of dengue viral proteins with host NMT and its subsequent effect on DENV. Our bioinformatics, molecular docking, and far\u2010western blotting analyses demonstrated the interaction of viral envelope protein (E) with NMT. The gene expression of NMT was strongly elevated in a dependent manner during the viral replication phase in dendritic cells. Moreover, NMT gene silencing significantly inhibited DENV replication in dendritic cells. Further studies investigating the target cell types of other host factors are suggested.<\/jats:p>","DOI":"10.1002\/mbo3.831","type":"journal-article","created":{"date-parts":[[2019,3,8]],"date-time":"2019-03-08T06:05:14Z","timestamp":1552025114000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Inhibition of <i>N<\/i>\u2010myristoyltransferase1 affects dengue virus replication"],"prefix":"10.1002","volume":"8","author":[{"given":"San","family":"Suwanmanee","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology Faculty of Tropical Medicine Mahidol University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuvadee","family":"Mahakhunkijcharoen","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Faculty of Tropical Medicine Mahidol University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumate","family":"Ampawong","sequence":"additional","affiliation":[{"name":"Department of Tropical Pathology Faculty of Tropical Medicine Mahidol University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pornsawan","family":"Leaungwutiwong","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Faculty of Tropical Medicine Mahidol University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doroth\u00e9e","family":"Miss\u00e9","sequence":"additional","affiliation":[{"name":"MIVEGEC UMR 224, Universit\u00e9 de Montpellier, IRD, CNRS Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7964-5298","authenticated-orcid":false,"given":"Natthanej","family":"Luplertlop","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Faculty of Tropical Medicine Mahidol University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,3,7]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2007.03.030"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-800098-4.00001-5"},{"key":"e_1_2_10_4_1","first-page":"e52065","article-title":"Viral concentration determination through plaque assays: Using traditional and novel overlay systems","volume":"93","author":"Baer A.","year":"2014","journal-title":"Journal of Visualzed Experiment"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.4103\/0974-777X.93761"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02761992000400017"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001625"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2009.12.032"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/cb5008647"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep37721"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2580625"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00354-15"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1010811107"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003632"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkj063"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0127661"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-011-1202-x"},{"key":"e_1_2_10_18_1","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1128\/jcm.30.3.545-551.1992","article-title":"Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase\u2010polymerase chain reaction","volume":"30","author":"Lanciotti R. 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