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The Chesapeake Bay (CB) and its upper tributaries (UT) is a large and biologically diverse estuary. This pilot study evaluated the presence of AR of gram\u2010negative bacteria isolated from water samples collected at various sites of the Chesapeake Bay. Bacterial organisms were identified and antimicrobial susceptibility testing was performed by phenotypic and genotypic methods. Ninety\u2010two distinctly different gram\u2010negative bacteria were identified; <jats:italic>Klebsiella pneumoniae<\/jats:italic>, <jats:italic>Enterobacter cloacae<\/jats:italic>, <jats:italic>Enterobacter aerogenes<\/jats:italic>, <jats:italic>Serratia marcescens<\/jats:italic>, and <jats:italic>Escherichia coli<\/jats:italic> were most often isolated. <jats:italic>Serratia marcescens <\/jats:italic>was more frequently isolated in samples from the UT compared to the CB. Antimicrobial resistance was more frequently detected in organisms from the CB by phenotypic and genotypic methods. Antimicrobial resistance to ampicillin, imipenem, tetracycline, and chloramphenicol were the most frequently observed resistance patterns. ACT\u20101, CMY, and SHV genes were the most frequently detected resistance genes, with predominance in organism isolated from the CB. The results from this study emphasize the importance for further developing comprehensive surveillance programs of AR in bacterial isolates in the various environments, such as recreational and other water systems.<\/jats:p>","DOI":"10.1002\/mbo3.839","type":"journal-article","created":{"date-parts":[[2019,4,5]],"date-time":"2019-04-05T15:49:31Z","timestamp":1554479371000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["A survey of antimicrobial resistance in Enterobacteriaceae isolated from the Chesapeake Bay and adjacent upper tributaries"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0573-4957","authenticated-orcid":false,"given":"Stefan","family":"Riedel","sequence":"first","affiliation":[{"name":"Department of Pathology Beth Israel Deaconess Medical Center Boston Massachusetts"},{"name":"Harvard Medical School Boston Massachusetts"},{"name":"Division of Microbiology, Department of Pathology, School of Medicine The Johns Hopkins University Baltimore Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Boire","sequence":"additional","affiliation":[{"name":"Division of Microbiology, Department of Pathology, School of Medicine The Johns Hopkins University Baltimore Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathryn A.","family":"Carson","sequence":"additional","affiliation":[{"name":"Department of Epidemiology Johns Hopkins Bloomberg School of Public Health Baltimore Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aravinda","family":"Vadlamudi","sequence":"additional","affiliation":[{"name":"Division of Microbiology, Department of Pathology, School of Medicine The Johns Hopkins University Baltimore Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua","family":"Khuvis","sequence":"additional","affiliation":[{"name":"Division of Microbiology, Department of Pathology, School of Medicine The Johns Hopkins University Baltimore Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vivek","family":"Vadlamudi","sequence":"additional","affiliation":[{"name":"Division of Microbiology, Department of Pathology, School of Medicine The Johns Hopkins University Baltimore Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vajini","family":"Atukorale","sequence":"additional","affiliation":[{"name":"Division of Microbiology, Department of Pathology, School of Medicine The Johns Hopkins University Baltimore Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria A. 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