{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T12:41:07Z","timestamp":1791722467164,"version":"4.3.4"},"reference-count":59,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,8,17]],"date-time":"2019-08-17T00:00:00Z","timestamp":1566000000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The results from this study revealed that crude extracts isolated from bacterial endophytes obtained from <jats:italic>Crinum macowanii<\/jats:italic> bulbs showed activity against both Gram\u2010positive and Gram\u2010negative pathogenic bacteria, while <jats:italic>Acinetobacter guillouiae<\/jats:italic> crude extracts displayed anticancer activity. This study aimed to isolate and characterize bacterial endophytes and their crude extracts from <jats:italic>C.\u00a0macowanii<\/jats:italic> bulbs. Endophytes were isolated using validated surface sterilization techniques, followed by phenotypic and genotypic profiles of the isolates. Crude extracts were extracted from the endophytes using ethyl acetate, while methanol:dichloromethane (1:1) was used to obtain crude extracts from the bulbs. Antibacterial activity of crude extract from each endophyte was investigated against selected pathogenic strains using the broth microdilution method, and anticancer activity against U87MG glioblastoma and A549 lung carcinoma cells was determined by the MTS (3\u2010(4,5\u2010dimethylthiazol\u20102\u2010yl)\u20105\u2010(3\u2010carboxymethoxy\u2010phenyl)\u20102\u2010(4\u2010sulfophenyl)\u20102H\u2010tetrazolium) assay. <jats:italic>Acinetobacter guillouiae<\/jats:italic>, <jats:italic>Pseudomonas moraviensis<\/jats:italic>, <jats:italic>Pseudomonas<\/jats:italic> sp., <jats:italic>Rahnella aquatilis<\/jats:italic>, <jats:italic>Bacillus cereus<\/jats:italic>, <jats:italic>Novosphingobium<\/jats:italic> sp., <jats:italic>Raoultella ornithinolytica<\/jats:italic>, and <jats:italic>Burkholderia tropica<\/jats:italic> were successfully isolated. The crude extracts from the majority of endophytes showed antibacterial activity, ranging from 0.125 to &gt;16.00\u00a0mg\/ml against Gram\u2010negative and Gram\u2010positive pathogenic bacteria. <jats:italic>Acinetobacter guillouiae<\/jats:italic> extracts showed a high bioactive potential against U87MG glioblastoma cell lines by reducing their growth by 50% at concentrations of 12.5, 6.25, and 3.13\u00a0\u00b5g\/ml. Crude extracts isolated from <jats:italic>C<\/jats:italic>.<jats:italic>\u00a0macowanii<\/jats:italic> bulbs showed potential for possible drug lead against common pathogenic bacteria.<\/jats:p>","DOI":"10.1002\/mbo3.914","type":"journal-article","created":{"date-parts":[[2019,8,17]],"date-time":"2019-08-17T07:09:22Z","timestamp":1566025762000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Evaluating antibacterial and anticancer activity of crude extracts of bacterial endophytes from <i>Crinum macowanii<\/i> Baker bulbs"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5245-5670","authenticated-orcid":false,"given":"Tendani E.","family":"Sebola","sequence":"first","affiliation":[{"name":"Department of Biotechnology and Food Technology, Faculty of Science University of Johannesburg Johannesburg South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9133-0624","authenticated-orcid":false,"given":"Nkemdinma C.","family":"Uche\u2010Okereafor","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Food Technology, Faculty of Science University of Johannesburg Johannesburg South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5161-6695","authenticated-orcid":false,"given":"Kudzanai I.","family":"Tapfuma","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Food Technology, Faculty of Science University of Johannesburg Johannesburg South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3069-4624","authenticated-orcid":false,"given":"Lukhanyo","family":"Mekuto","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Faculty of Engineering and the Built Environment University of Johannesburg Johannesburg South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4962-0724","authenticated-orcid":false,"given":"Ezekiel","family":"Green","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Food Technology, Faculty of Science University of Johannesburg Johannesburg South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4672-6176","authenticated-orcid":false,"given":"Vuyo","family":"Mavumengwana","sequence":"additional","affiliation":[{"name":"Division of Molecular Biology and Human Genetics, Department of Medicine and Health Sciences, South African Medical Research Council Centre for Tuberculosis Research Stellenbosch University Cape Town South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,8,17]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/48.suppl_1.5"},{"issue":"6","key":"e_1_2_11_3_1","first-page":"1","article-title":"Bacterial degradation of monocyclic aromatic amines","volume":"820","author":"Arora P. 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