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In this study, we describe the development and evaluation of a simple, sensitive, and specific loop\u2010mediated isothermal amplification (LAMP) assay for the detection of <jats:italic>Chlamydia pecorum<\/jats:italic> in koalas as a point\u2010of\u2010care diagnostic tool that can be used in any wildlife hospital and in the field on specialized instrumentation. A set of primers targeting a 188\u2010bp region of the <jats:italic>C.\u00a0pecorum<\/jats:italic> genome was designed. 100% specificity of the LAMP assay was revealed by demonstrating no cross\u2010reactivity with 33 nontarget pathogens, and complete correlation with qPCR results for 43 clinical swabs collected opportunistically from wildlife hospitals. In sensitivity evaluations, the technique successfully detected serial dilutions of extracted <jats:italic>C. pecorum<\/jats:italic> DNA with a detection limit of 44 IFU\/ml.<\/jats:p>","DOI":"10.1002\/mbo3.916","type":"journal-article","created":{"date-parts":[[2019,8,16]],"date-time":"2019-08-16T13:04:11Z","timestamp":1565960651000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Rapid point\u2010of\u2010care diagnostics for the detection of <i>Chlamydia pecorum<\/i> in koalas (<i>Phascolarctos cinereus<\/i>) using loop\u2010mediated isothermal amplification without nucleic acid purification"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5701-7175","authenticated-orcid":false,"given":"Lyndal S.","family":"Hulse","sequence":"first","affiliation":[{"name":"School of Agriculture and Food Sciences University of Queensland Gatton QLD 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