{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T11:09:30Z","timestamp":1790766570587,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,9,26]],"date-time":"2019-09-26T00:00:00Z","timestamp":1569456000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81372869"],"award-info":[{"award-number":["81372869"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81572628"],"award-info":[{"award-number":["81572628"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Ankylosing spondylitis is a chronic, progressive disease, and its treatment is relevant to the gut microbiota. Anti\u2010tumor necrosis factor\u2010alpha (anti\u2010TNF\u2010\u03b1) therapy alters the gut microbiota in many diseases, including inflammatory bowel disease. However, little is known about the effect of TNF\u2010\u03b1 blocker treatment on the gut microbiota in ankylosing spondylitis. Herein, the effect of a TNF\u2010\u03b1 blocker on the gut microbiota in proteoglycan\u2010induced arthritis was investigated. Proteoglycan\u2010induced mice were treated with an rhTNFR:Fc solution of etanercept (5\u00a0\u00b5g\/g) for 4\u00a0weeks. rhTNFR:Fc treatment attenuated the arthritis incidence and severity of arthritis in the proteoglycan\u2010induced mice and decreased inflammation in the ankle joints and ameliorated ileal tissue destruction. Moreover, high gut permeability occurred, and zonula occludens\u20101 and occludin protein levels were reduced in proteoglycan\u2010induced mice. These levels were significantly restored by the administration of rhTNFR:Fc. The serum TNF\u2010\u03b1 and IL\u201017 levels were also decreased. In addition, flora analysis via 16S rDNA high\u2010throughput sequencing revealed that rhTNFR:Fc treatment restored the gut microbiota composition to a composition similar to that in control mice. In conclusion, anti\u2010TNF\u2010\u03b1 therapy attenuated proteoglycan\u2010induced arthritis progression and modulated the gut microbiota and intestinal barrier function. These results provide new insights for anti\u2010TNF\u2010\u03b1 therapy strategies via regulating the gut microbiota in ankylosing spondylitis.<\/jats:p>","DOI":"10.1002\/mbo3.927","type":"journal-article","created":{"date-parts":[[2019,9,26]],"date-time":"2019-09-26T08:41:26Z","timestamp":1569487286000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Anti\u2010TNF\u2010\u03b1 therapy alters the gut microbiota in proteoglycan\u2010induced ankylosing spondylitis in mice"],"prefix":"10.1002","volume":"8","author":[{"given":"Bin","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Spine surgery The Fifth Affiliated Hospital, Sun Yat\u2010sen University Zhuhai Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lianjun","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Spine surgery The Fifth Affiliated Hospital, Sun Yat\u2010sen University Zhuhai Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhifei","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Spine surgery The Fifth Affiliated Hospital, Sun Yat\u2010sen University Zhuhai Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junchi","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Academy of Orthopedics of Guangdong Province, Orthopaedic Hospital of Guangdong Province, The Third Affiliated Hospital Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jincheng","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, Henan Provincial People's Hospital Zhengzhou University People's Hospital Zhengzhou Henan 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Surgery, Academy of Orthopedics of Guangdong Province, Orthopaedic Hospital of Guangdong Province, The Third Affiliated Hospital Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinshi","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Academy of Orthopedics of Guangdong Province, Orthopaedic Hospital of Guangdong Province, The Third Affiliated Hospital Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingxuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Academy of Orthopedics of Guangdong Province, Orthopaedic Hospital of Guangdong Province, The Third Affiliated Hospital Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingchu","family":"Li","sequence":"additional","affiliation":[{"name":"Department of 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