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Another important target of EPEC is the tight junction (TJ), and EspF induces displacement of TJ proteins and increased intestinal permeability. Previously, we determined that an EPEC strain lacking EspF did not cause TJ disruption; meanwhile, pedestals were located on the TJ and smaller than those induced by the wild\u2010type strain. Therefore, EspF could be playing an important role in both phenotypes. Here, using different cell models, we found that EspF was essential for pedestal maturation through ZO\u20101 disassembly from TJ, leading to (a) ZO\u20101 recruitment to the pedestal structure; no other main TJ proteins were required. Recruited ZO\u20101 allowed the afadin recruitment. (b) Afadin recruitment caused an afadin\u2013ZO\u20101 transient interaction, like during TJ formation. (c) Afadin and ZO\u20101 were segregated to the tip and the stem of pedestal, respectively, causing pedestal maturation. Initiation of these three discrete phases for pedestal maturation functionally and physically required EspF expression. Pedestal maturation process could help coordinate the epithelial actomyosin function by maintaining the actin\u2010rich column composing the pedestal structure and could be important in the dynamics of the pedestal movement on epithelial cells.<\/jats:p>","DOI":"10.1002\/mbo3.931","type":"journal-article","created":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T05:30:05Z","timestamp":1569907805000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Coordinated transient interaction of ZO\u20101 and afadin is required for pedestal maturation induced by EspF from enteropathogenic <i>Escherichia coli<\/i>"],"prefix":"10.1002","volume":"8","author":[{"given":"Paul","family":"Ugalde\u2010Silva","sequence":"first","affiliation":[{"name":"Department of Cell Biology Centro de Investigaci\u00f3n y de Estudios Avanzados del IPN (CINVESTAV\u2010IPN) M\u00e9xico City 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