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Producer organisms are protected from their bacteriocin by a dedicated immunity protein. The immunity proteins for two\u2010peptide bacteriocins contain putative transmembrane domains (TMDs) and might therefore be associated with the membrane. The immunity protein CbnZ for the two\u2010peptide bacteriocin carnobacteriocin XY (CbnXY) was identified by heterologously expressing the <jats:italic>cbnZ<\/jats:italic> gene in sensitive host strains. Using protein topology prediction methods and the dual <jats:italic>pho\u2010lac<\/jats:italic> reporter system, we mapped out the membrane topology of CbnZ, along with those of the immunity proteins LagC and LciM for the two\u2010peptide bacteriocins lactococcin G and lactococcin MN, respectively. Our results reveal wide structural variety between these immunity proteins that can contain as little as one TMD or as many as four TMDs.<\/jats:p>","DOI":"10.1002\/mbo3.957","type":"journal-article","created":{"date-parts":[[2019,10,31]],"date-time":"2019-10-31T04:29:09Z","timestamp":1572496149000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["The membrane topology of immunity proteins for the two\u2010peptide bacteriocins carnobacteriocin XY, lactococcin G, and lactococcin MN shows structural diversity"],"prefix":"10.1002","volume":"9","author":[{"given":"Angelle P.","family":"Britton","sequence":"first","affiliation":[{"name":"Department of Chemistry The King's University Edmonton AB Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah R.","family":"van der Ende","sequence":"additional","affiliation":[{"name":"Department of Chemistry The King's University Edmonton AB Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco J.","family":"van Belkum","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Alberta Edmonton AB Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7307-9328","authenticated-orcid":false,"given":"Leah A.","family":"Martin\u2010Visscher","sequence":"additional","affiliation":[{"name":"Department of Chemistry The King's University Edmonton AB Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,10,30]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fuy033"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/1873-3468.12648"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/aem.56.8.2503-2510.1990"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-016-7343-9"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12915-017-0404-4"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2015.07.011"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1605(01)00560-8"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1240"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4419-7692-5_19"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.05576-11"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-38518-6"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0185279"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2008.05.003"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-148-4-973"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/mbo3.827"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00194-12"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02312-14"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2018.10.007"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-7033-9_10"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12632"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01553-09"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0196-9781(01)00494-6"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12010-015-1786-9"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/386410"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.12.4757-4766.1998"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781118868386.ch5"},{"key":"e_1_2_10_28_1","first-page":"645","article-title":"Genetics of non\u2010lantibiotic bacteriocins","volume":"85","author":"Nes I. 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