{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:12:57Z","timestamp":1772118777526,"version":"3.50.1"},"reference-count":52,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,11,19]],"date-time":"2019-11-19T00:00:00Z","timestamp":1574121600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010654","name":"Universidad San Francisco de Quito","doi-asserted-by":"publisher","award":["HUBI12441"],"award-info":[{"award-number":["HUBI12441"]}],"id":[{"id":"10.13039\/501100010654","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["MicrobiologyOpen"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Probiotic bacteria are frequently used to treat intestinal diseases or to improve health; however, little is known about the evolutionary changes of these bacteria during probiotic manufacture and the bacterial ability to colonize the intestine. It has been observed that when bacteria adapt to a new environment, they lose some traits required to thrive in the original niche. In this study, a strain of\n                    <jats:italic>Lactobacillus reuteri<\/jats:italic>\n                    was isolated from mouse duodenum and subjected to 150 serial passes in milk to simulate the industrial propagation of probiotic bacteria. The strains adapted to milk outperformed their ancestor when grown in milk; we also showed evidence of reduced intestinal colonization of milk\u2010adapted strains. Whole\u2010genome sequencing showed that bacterial adaptation to milk selects mutants with altered metabolic functions.\n                  <\/jats:p>","DOI":"10.1002\/mbo3.972","type":"journal-article","created":{"date-parts":[[2019,11,20]],"date-time":"2019-11-20T01:53:50Z","timestamp":1574214830000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Evolutionary changes of an intestinal\n                    <i>Lactobacillus reuteri<\/i>\n                    during probiotic manufacture"],"prefix":"10.1002","volume":"9","author":[{"given":"L\u00e1zaro","family":"L\u00f3pez","sequence":"first","affiliation":[{"name":"Instituto de Microbiolog\u00eda Colegio de Ciencias Biol\u00f3gicas y Ambientales Universidad San Francisco de Quito Quito Ecuador"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diana","family":"Calder\u00f3n","sequence":"additional","affiliation":[{"name":"Instituto de Microbiolog\u00eda Colegio de Ciencias Biol\u00f3gicas y Ambientales Universidad San Francisco de Quito Quito Ecuador"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9626-4489","authenticated-orcid":false,"given":"Pa\u00fal","family":"Cardenas","sequence":"additional","affiliation":[{"name":"Instituto de Microbiolog\u00eda Colegio de Ciencias Biol\u00f3gicas y Ambientales Universidad San Francisco de Quito Quito Ecuador"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda B.","family":"Prado","sequence":"additional","affiliation":[{"name":"Instituto de Microbiolog\u00eda Colegio de Ciencias Biol\u00f3gicas y Ambientales Universidad San Francisco de Quito Quito Ecuador"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos","family":"Valle","sequence":"additional","affiliation":[{"name":"Instituto de Microbiolog\u00eda Colegio de Ciencias Biol\u00f3gicas y Ambientales Universidad San Francisco de Quito Quito Ecuador"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2617-9021","authenticated-orcid":false,"given":"Gabriel","family":"Trueba","sequence":"additional","affiliation":[{"name":"Instituto de Microbiolog\u00eda Colegio de Ciencias Biol\u00f3gicas y Ambientales Universidad San Francisco de Quito Quito Ecuador"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,11,19]]},"reference":[{"key":"e_1_2_10_2_1","volume-title":"Current protocols in molecular biology","author":"Ausubel F. M.","year":"1987"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2008-1457"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0102-09352009000200006"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature18849"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/mec.13979"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-0-387-21609-6"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0911253107"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1001790"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.2289704"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0011147"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3920\/BM2016.0146"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200700974"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0020338"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aat2456"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.1991.tb00453.x"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2014.66"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1003617"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-016-1016-1"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000002658"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.11.4949-4956.1999"},{"key":"e_1_2_10_22_1","volume-title":"Handbook of milk composition","author":"Jensen R. G.","year":"1995"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yrtph.2012.04.003"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tree.2012.06.001"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSystems.00192-16"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msw054"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2015.2292"},{"key":"e_1_2_10_28_1","first-page":"916","article-title":"Enhanced production of bacitracin by knocking out of amino acid permease gene yhdG in Bacillus licheniformis DW2","volume":"34","author":"Li Y.","year":"2018","journal-title":"Sheng Wu Gong Cheng Xue Bao"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0307195101"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0043910"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.8.2481-2486.2004"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3002701"},{"key":"e_1_2_10_33_1","volume-title":"A short course in bacterial genetics: A laboratory manual and handbook for Escherichia coli and related bacteria","author":"Miller J. H.","year":"1992"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M011041200"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2009.123"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.03.003"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.10.4.696-701.2003"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.127.2.679-690.1976"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1996.tb03535.x"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/evo.13569"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1403169"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu153"},{"key":"e_1_2_10_43_1","volume-title":"snippy: Fast bacterial variant calling from NGS reads","author":"Seemann T.","year":"2015"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/237921"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2005.01228.x"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature18959"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.01994.x"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.2.1176-1181.2004"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0081882"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt1099"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1080\/07315724.2006.10719554"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0126210"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0278-6915(98)00090-8"}],"container-title":["MicrobiologyOpen"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mbo3.972","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/mbo3.972","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mbo3.972","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T04:14:26Z","timestamp":1694146466000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mbo3.972"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11,19]]},"references-count":52,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2020,2]]}},"alternative-id":["10.1002\/mbo3.972"],"URL":"https:\/\/doi.org\/10.1002\/mbo3.972","archive":["Portico"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/567974","asserted-by":"object"}]},"ISSN":["2045-8827","2045-8827"],"issn-type":[{"value":"2045-8827","type":"print"},{"value":"2045-8827","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,11,19]]},"assertion":[{"value":"2019-09-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-10-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-11-19","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e972"}}