{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T22:04:24Z","timestamp":1790460264479,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2002,2,20]],"date-time":"2002-02-20T00:00:00Z","timestamp":1014163200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Carcinogenesis"],"published-print":{"date-parts":[[2002,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The tuberous sclerosis (<jats:italic>TSC<\/jats:italic>) 2 tumor suppressor gene encodes the protein tuberin, which has recently been shown to play a crucial role in the intracellular trafficking of polycystin\u20101, the product of the polycystic kidney disease (<jats:italic>PDK<\/jats:italic>) 1 gene. <jats:italic>PKD1<\/jats:italic> is responsible for most cases of autosomal dominant polycystic kidney disease, which has been described as \u201cneoplasia in disguise.\u201d Polycystin\u20101 is a membrane protein localized to adherens junctions in a complex containing E\u2010cadherin and \u03b1\u2010, \u03b2\u2010, and \u03b3\u2010catenins. To determine whether loss of membrane localization of polycystin\u20101 and E\u2010cadherin affects the function of \u03b2\u2010catenin, \u03b2\u2010catenin localization and signaling were characterized in tuberin\u2010null EKT2 and ERC15 cells and in tuberin\u2010positive TRKE2 cells derived from polycystic, neoplastic, and normal rat kidney epithelial cells, respectively. EKT2 cells lacking tuberin because of inactivation of the <jats:italic>Tsc2<\/jats:italic> gene fail to localize polycystin\u20101 and E\u2010cadherin appropriately to these junctions. However, \u03b2\u2010catenin was retained at lateral cell membranes in both tuberin\u2010null and tuberin\u2010positive cells. Moreover, gene transcription mediated by \u03b2\u2010catenin T\u2010cell\u2013specific transcription factor complexes showed no differences among EKT2, ERC15, and TRKE2 cells. Thus, \u03b2\u2010catenin was stably retained at the lateral cell membrane in tuberin\u2010null renal cells lacking membrane\u2010localized polycystin\u20101 and E\u2010cadherin. These data suggest that, although loss of <jats:italic>Tsc2<\/jats:italic> tumor suppressor gene function disrupts normal polycystin\u20101 function and membrane localization of E\u2010cadherin, normal \u03b2\u2010catenin signaling is retained in tuberin\u2010null cells. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mc.10034","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T23:07:59Z","timestamp":1030316879000},"page":"131-136","source":"Crossref","is-referenced-by-count":19,"title":["Retention of membrane\u2010localized \u03b2\u2010catenin in cells lacking functional polycystin\u20101 and tuberin"],"prefix":"10.1002","volume":"33","author":[{"given":"Hiroyuki","family":"Kugoh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Kleymenova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheryl Lyn","family":"Walker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,2,20]]},"reference":[{"key":"e_1_2_6_2_2","volume-title":"Developmental perspectives in psychiatry","author":"Gomez MR","year":"1999"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0142(19981115)83:10<2208::AID-CNCR21>3.0.CO;2-K"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-199802000-00005"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90618-Z"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90137-6"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199307293290508"},{"key":"e_1_2_6_8_2","doi-asserted-by":"crossref","first-page":"1841","DOI":"10.1681\/ASN.V3121841","article-title":"1992 Homer Smith Award. 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