{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,30]],"date-time":"2024-05-30T05:07:19Z","timestamp":1717045639889},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2003,1,28]],"date-time":"2003-01-28T00:00:00Z","timestamp":1043712000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Carcinogenesis"],"published-print":{"date-parts":[[2003,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Alteration of the <jats:italic>Fhit<\/jats:italic> gene was investigated in pancreatic duct adenocarcinomas induced by <jats:italic>N<\/jats:italic>\u2010nitrosobis(2\u2010oxopropyl)amine (BOP) in Syrian golden hamsters. The animals received 70 mg\/kg BOP, followed by repeated exposure to an augmentation pressure regimen consisting of a choline\u2010deficient diet combined with <jats:sc>DL<\/jats:sc>\u2010ethionine and then <jats:sc>L<\/jats:sc>\u2010methionine and administration of 20 mg\/kg BOP. A total of 15 pancreatic duct adenocarcinomas were obtained 10 wk after the beginning of the experiment, and total RNAs were extracted from each for assessment of aberrant transcription of the <jats:italic>Fhit<\/jats:italic> gene by reverse transcription\u2013polymerase chain reaction analysis. Aberrant transcripts lacking nucleotides in the regions of nt \u221275 to 348, nt \u221215 to 348, or nt \u221275 to 178 were detected in 11 adenocarcinomas (73.3%). Southern blot analysis of eight tumors did not show any evidence of gross rearrangement or deletion. These results indicated that changes in the <jats:italic>Fhit<\/jats:italic> gene occurred frequently and thus may have played a role in the development of pancreatic duct adenocarcinomas induced by BOP in hamsters. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mc.10099","type":"journal-article","created":{"date-parts":[[2003,1,30]],"date-time":"2003-01-30T14:01:44Z","timestamp":1043935304000},"page":"60-66","source":"Crossref","is-referenced-by-count":14,"title":["Alterations in the <i>Fhit<\/i> gene in pancreatic duct adenocarcinomas induced by <i>N<\/i>\u2010nitrosobis(2\u2010oxopropyl)amine in hamsters"],"prefix":"10.1002","volume":"36","author":[{"given":"Toshifumi","family":"Tsujiuchi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasutaka","family":"Sasaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tadahiko","family":"Kubozoe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoichi","family":"Konishi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Tsutsumi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,1,28]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/91.8.675"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006676-199804000-00006"},{"key":"e_1_2_6_4_2","first-page":"349","article-title":"Cancer 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