{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T11:03:57Z","timestamp":1779275037575,"version":"3.51.4"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2003,5,19]],"date-time":"2003-05-19T00:00:00Z","timestamp":1053302400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Carcinogenesis"],"published-print":{"date-parts":[[2003,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We investigated the effects of overexpression of the tuberous sclerosis\u20101 and \u20102 (<jats:italic>TSC1\/2<\/jats:italic>) gene products (hamartin and tuberin, respectively) in the human kidney epithelial cell line 293 with an inducible expression system. As we had observed previously in fibroblasts, 293 cells overexpressing hamartin and\/or tuberin grew more slowly in vitro. However, here we also observed that the 293 overexpressing cells underwent a dramatic morphological change in which groups of cells formed compact clusters. The overexpressing cells also displayed decreased dissociation and increased reaggregation in vitro. These changes were found to be associated with an increased level of E\u2010cadherin, which is known to regulate cell\u2010cell interactions in epithelial cells, and of its binding partner \u03b2\u2010catenin. Consistent with the role of E\u2010cadherin in these effects, we found that the observed changes in 293 cell morphology, dissociation, and adhesion were calcium\u2010dependent, and were reproduced by overexpression of E\u2010cadherin. In contrast, overexpression of <jats:italic>TSC1<\/jats:italic> in rat embryo fibroblasts, which lack E\u2010cadherin, failed to elicit the same changes as in 293 cells. We conclude that the hamartin\/tuberin complex exerted a direct effect on the morphology and adhesive properties of 293 cells through regulation of the level and\/or activity of cellular E\u2010cadherin\/\u03b2\u2010catenin. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mc.10125","type":"journal-article","created":{"date-parts":[[2003,5,21]],"date-time":"2003-05-21T10:02:10Z","timestamp":1053511330000},"page":"98-109","source":"Crossref","is-referenced-by-count":9,"title":["Regulation of cell morphology and adhesion by the tuberous sclerosis complex (<i>TSC1\/2<\/i>) gene products in human kidney epithelial cells through increased E\u2010cadherin\/\u03b2\u2010catenin activity"],"prefix":"10.1002","volume":"37","author":[{"given":"Shaowei","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Braverman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongzhen","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William C.","family":"Vass","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas R.","family":"Lowy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey E.","family":"DeClue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,5,19]]},"reference":[{"key":"e_1_2_7_2_2","volume-title":"Developmental perspectives in psychiatry","author":"Gomez MR","year":"1999"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004390000348"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1177\/088307389801301206"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.23.10914"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90618-Z"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.277.5327.805"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/7.6.1053"},{"key":"e_1_2_7_9_2","first-page":"4766","article-title":"Hamartin, the product of the tuberous sclerosis 1 (TSC1) gene, interacts with tuberin and appears to be localized to cytoplasmic vessicles","volume":"58","author":"Plank TL","year":"1998","journal-title":"Cancer Res"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.50.35647"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-4547(20010201)63:3<276::AID-JNR1021>3.0.CO;2-A"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/10.25.2899"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/10.25.2889"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C100136200"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00333-6"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00332-4"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.901101"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-8049(00)00158-1"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.10083"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2168.2000.01513.x"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI0215429"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004280100516"},{"key":"e_1_2_7_23_2","first-page":"1201","article-title":"Tuberous sclerosis\u2010associated renal cell carcinoma","volume":"149","author":"Bjornsson J","year":"1996","journal-title":"Am J Pathol"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204009"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/16.1.369"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.153.5.1049"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.278.5342.1464"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.17.9154"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.142.4.1105"},{"key":"e_1_2_7_30_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.149.2.457"},{"key":"e_1_2_7_31_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1998.8714"},{"key":"e_1_2_7_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/9.12.1721"},{"key":"e_1_2_7_33_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1996.0183"},{"key":"e_1_2_7_34_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnen\/61.2.154"},{"key":"e_1_2_7_35_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/11.5.525"},{"key":"e_1_2_7_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/35010550"},{"key":"e_1_2_7_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb758"},{"key":"e_1_2_7_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(01)00226-X"},{"key":"e_1_2_7_39_2","doi-asserted-by":"publisher","DOI":"10.1002\/mc.10034"}],"container-title":["Molecular Carcinogenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fmc.10125","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mc.10125","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T13:57:26Z","timestamp":1695909446000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mc.10125"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,5,19]]},"references-count":38,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2003,6]]}},"alternative-id":["10.1002\/mc.10125"],"URL":"https:\/\/doi.org\/10.1002\/mc.10125","archive":["Portico"],"relation":{},"ISSN":["0899-1987","1098-2744"],"issn-type":[{"value":"0899-1987","type":"print"},{"value":"1098-2744","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,5,19]]}}}