{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T07:37:23Z","timestamp":1791185843096,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2003,11,19]],"date-time":"2003-11-19T00:00:00Z","timestamp":1069200000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Carcinogenesis"],"published-print":{"date-parts":[[2003,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Different mechanisms, such as chromosomal rearrangements, deletions, mutations, and methylation\/demethylation of the promoter regions of genes, have been shown to be involved in acute lymphoblastic leukaemia (ALL). These genetic and epigenetic alterations lead to the activation of protooncogenes or to inactivation of tumour supressor genes promoting cell proliferation. One of the most frequently inactivated tumour supressor genes is <jats:italic>TP53<\/jats:italic>, which is altered in 50% of human tumours. In this study, we have analysed: (1) the complete coding region, all intron\u2010exon junctions and noncoding regions of exons 1\u201311 of <jats:italic>TP53<\/jats:italic> by <jats:italic>lexon<\/jats:italic>\u2010DGGE; (2) the methylation status of the 5\u2032 region of <jats:italic>TP53<\/jats:italic> and (3) the deletion of one or both alleles of the gene by fluorescence in situ hybridisation (FISH) in 57 ALL patients. Using these techniques, we have found promoter methylation in 32% of the cases, missense mutations in 8.8%, and deletion of one allele in 7.5% of the samples, with <jats:italic>TP53<\/jats:italic> being altered in 40% of the ALL samples studied in this series. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mc.10159","type":"journal-article","created":{"date-parts":[[2003,11,20]],"date-time":"2003-11-20T18:19:07Z","timestamp":1069352347000},"page":"201-208","source":"Crossref","is-referenced-by-count":62,"title":["<i>TP53<\/i> is frequently altered by methylation, mutation, and\/or deletion in acute lymphoblastic leukaemia"],"prefix":"10.1002","volume":"38","author":[{"given":"Xabier","family":"Agirre","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco J.","family":"Novo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda 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