{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T22:43:33Z","timestamp":1791153813862,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2001,5,22]],"date-time":"2001-05-22T00:00:00Z","timestamp":990489600000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Carcinogenesis"],"published-print":{"date-parts":[[2001,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The low\u2010affinity nerve growth factor receptor p75<jats:sup>NTR<\/jats:sup> is a 75\u2010kDa glycoprotein that belongs to the tumor necrosis factor receptor superfamily and has been implicated in the induction of apoptosis in various tissues and cell lines. Immunohistochemistry on tissue sections from radical prostatectomies has shown that expression of p75<jats:sup>NTR<\/jats:sup> is limited to the epithelial cells. Western blot and immunohistochemical analyses have also shown a progressive loss of p75<jats:sup>NTR<\/jats:sup> expression in prostate epithelial cells during the malignant progression of organ\u2010confined adenocarcinomas, with complete loss of expression in the naturally occurring prostate tumor cell lines DU\u2010145, PC\u20103, LNCaP, and TSU\u2010pr1, which were derived from metastases. Reintroduction of p75<jats:sup>NTR<\/jats:sup> expression into the TSU\u2010pr1 tumor cell line was shown to reestablish the ability of these cells to undergo p75<jats:sup>NTR<\/jats:sup>\u2010mediated apoptosis. It is not known whether this loss of expression is due to deletion of part or the entire p75<jats:sup>NTR<\/jats:sup> gene or to other factors. Through the use of southern blotting and polymerase chain reaction (PCR), we showed that loss of p75<jats:sup>NTR<\/jats:sup> protein expression was not due to deletion or loss of the gene. Furthermore, through reverse transcription\u2013PCR, RNase protection, and the chromatin immunoprecipitation assay, we showed that transcription of the p75<jats:sup>NTR<\/jats:sup> gene occurred in these prostate tumor cell lines. Finally, through transient transfection using two constructs of p75<jats:sup>NTR<\/jats:sup>, one containing the full 2\u2010kb 3\u2032 untranslated region and one that contains only a few hundred bases of the 3\u2032 untranslated region (UTR), we showed that the 3\u2032 UTR may have a role in the loss of p75<jats:sup>NTR<\/jats:sup> expression in prostate cancer. \u00a9 2001 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mc.1038","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T20:35:44Z","timestamp":1030307744000},"page":"46-55","source":"Crossref","is-referenced-by-count":40,"title":["Molecular characterization of the loss of p75<sup>NTR<\/sup> expression in human prostate tumor cells"],"prefix":"10.1002","volume":"31","author":[{"given":"Scott","family":"Krygier","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Djakiew","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2001,5,22]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.3322\/canjclin.48.1.6"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0045(20000201)42:2<150::AID-PROS10>3.0.CO;2-H"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3008331"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/325593a0"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8128229"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/383166a0"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1200972"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/roi.2970030617"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0045(19970301)30:4<274::AID-PROS8>3.0.CO;2-L"},{"key":"e_1_2_7_11_2","first-page":"5403","article-title":"Reduced 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