{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T16:05:25Z","timestamp":1764259525558},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,5,3]],"date-time":"2005-05-03T00:00:00Z","timestamp":1115078400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Carcinogenesis"],"published-print":{"date-parts":[[2005,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Immortalization is an early and essential step of human carcinogenesis. Amplification of chromosome 20q has been shown to be a common event in immortalized cells and cancers. We have previously reported that gain and amplification of chromosome 20q is a non\u2010random and common event in immortalized human ovarian surface epithelial (HOSE) cells. The chromosome 20q harbors genes including <jats:italic>TGIF2<\/jats:italic> (20q11.2\u2010q12), <jats:italic>AIB1<\/jats:italic> (20q12), <jats:italic>PTPN1<\/jats:italic> (20q13.1), <jats:italic>ZNF217<\/jats:italic> (20q13.2), and <jats:italic>AURKA<\/jats:italic> (20q13.2\u2010q13.3), which were previously reported to be amplified and overexpressed in ovarian cancers. Some of these genes may be involved in immortalization of HOSE cells and represent crucial premalignant changes in ovarian surface epithelium. Investigation of the involvement of these genes was examined in four pairs of pre\u2010crisis (preimmortalized) and post\u2010crisis (immortalized) HOSE cells. Overexpression of <jats:italic>AURKA<\/jats:italic> (<jats:italic>Aurora kinase A<\/jats:italic>), also known as BTAK and STK15, by both real time\u2010quantitative polymerase chain reaction (RT\u2010QPCR) and Western blotting was detected in all the four immortalized HOSE cells examined while overexpression of <jats:italic>AIB1<\/jats:italic> and <jats:italic>ZNF217<\/jats:italic> was observed in two of four immortalized HOSE cells examined. Overexpression of <jats:italic>TGIF2<\/jats:italic> and <jats:italic>PTPN1<\/jats:italic> was not significant in our immortalized HOSE cell systems. The degree of overexpression of <jats:italic>AURKA<\/jats:italic> was shown to be closely associated with the amplification of chromosome 20q in immortalized HOSE cells. Fluorescence in situ hybridization (FISH) with labeled P1 artificial clone (PAC) confirmed the amplification of the chromosomal region (20q13.2\u201013.3) where <jats:italic>AURKA<\/jats:italic> resides. DNA amplification of <jats:italic>AURKA<\/jats:italic> was also confirmed using semi\u2010quantitative PCR. Our study showed that amplification and overexpression of <jats:italic>AURKA<\/jats:italic> is a common and significant event during immortalization of HOSE cells and may represent an important premalignant change in ovarian carcinogenesis. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mc.20098","type":"journal-article","created":{"date-parts":[[2005,5,6]],"date-time":"2005-05-06T19:48:07Z","timestamp":1115408887000},"page":"165-174","source":"Crossref","is-referenced-by-count":39,"title":["Amplification and overexpression of Aurora kinase A (AURKA) in immortalized human ovarian epithelial (HOSE) cells"],"prefix":"10.1002","volume":"43","author":[{"given":"C.M.","family":"Chung","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Man","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Jin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Jin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X.Y.","family":"Guan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Q.","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.S.K.","family":"Wan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.L.M.","family":"Cheung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.W.","family":"Tsao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,5,3]]},"reference":[{"key":"e_1_2_7_2_2","volume-title":"Cancer 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