{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T22:23:28Z","timestamp":1778711008155,"version":"3.51.4"},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,11,7]],"date-time":"2006-11-07T00:00:00Z","timestamp":1162857600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Carcinogenesis"],"published-print":{"date-parts":[[2007,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In this study we explored the mutation types of <jats:italic>p16<jats:sup>CDKN2A<\/jats:sup><\/jats:italic> exon 1 and the corresponding frequencies in experimental rat tongue carcinogenesis. Twenty barrier Sprague\u2013Dawley (SD) rats were divided into the control (<jats:italic>n<\/jats:italic>\u2009=\u20095) and experimental group (<jats:italic>n<\/jats:italic>\u2009=\u200915), to which 4\u2010nitroquinoline\u20101\u2010oxide (4\u2010NQO) in drinking water was administered. Two samples of normal, three samples of moderate\/severe dysplasia and four samples of invasive squamous cell carcinoma lesions were selected following strict histopathological examination in double\u2010blind manner. The PCR products of <jats:italic>p16<jats:sup>CDKN2A<\/jats:sup><\/jats:italic> exon 1 amplified from these tissues were sequenced. Point mutations of <jats:italic>p16<jats:sup>CDKN2A<\/jats:sup><\/jats:italic> exon 1 were found in all of the precancerous and cancerous lesions. Half of the mutations were detected on guanine (G). Twenty mutations, including a missense mutation of the start codon resulting in alternative reading frame of <jats:italic>p16<jats:sup>CDKN2A<\/jats:sup><\/jats:italic> exon 1, were also identified. These preliminary results suggested that mutation of <jats:italic>p16<jats:sup>CDKN2A<\/jats:sup><\/jats:italic> exon 1 might be an early molecular event of rat tongue carcinogenesis induced by 4NQO and G was the mutation hotspot. \u00a9 2006 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mc.20197","type":"journal-article","created":{"date-parts":[[2006,11,7]],"date-time":"2006-11-07T14:46:40Z","timestamp":1162910800000},"page":"85-90","source":"Crossref","is-referenced-by-count":11,"title":["Frequent mutation of <i>p16<sup>CDKN2A<\/sup><\/i> exon 1 during rat tongue carcinogenesis induced by 4\u2010nitroquinoline\u20101\u2010oxide"],"prefix":"10.1002","volume":"46","author":[{"given":"Yun","family":"Hong","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linglan","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunyang","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongbin","family":"Xia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nelson L.","family":"Rhodus","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,11,7]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.MP.0000085760.74313.DD"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8153634"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1998.16.3.1197"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/emm.2004.23"},{"key":"e_1_2_6_6_2","first-page":"325","article-title":"Detection of p16CDKN2A exon1 methylation in experimental tongue carcinogenesis in rats","volume":"19","author":"Cheng B","year":"2003","journal-title":"J Pract 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precancerous lesions and squamous cell carcinoma of oral mucosa","volume":"38","author":"Wang WX","year":"2003","journal-title":"Zhonghua Kou Qiang Yi Xue Za Zhi"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.17.3.1366"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1089\/104454904322964760"}],"container-title":["Molecular 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