{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:29:14Z","timestamp":1762100954958},"reference-count":26,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2009,7,6]],"date-time":"2009-07-06T00:00:00Z","timestamp":1246838400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Carcinogenesis"],"published-print":{"date-parts":[[2009,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The <jats:italic>MSH6<\/jats:italic> G39E germline polymorphism is not associated with an increased risk of either microsatellite stable or unstable sporadic colorectal cancer. Other than microsatellite instability, however, most genetic and epigenetic changes of tumors associated with this common variant have not been studied. The objective of our investigation was to evaluate associations between the <jats:italic>MSH6<\/jats:italic> G39E (116G&gt;A) polymorphism and CpG island methylator phenotype (CIMP) and <jats:italic>BRAF<\/jats:italic> V600E mutations in tumors from a sample of 1048 individuals with colon cancer and 1964 controls from Utah, Northern California, and Minnesota. The G39E polymorphism (rs1042821) was determined by the five prime nuclease assay. CIMP was determined by methylation\u2010specific polymerase chain reaction (PCR) of CpG islands in <jats:italic>MLH1<\/jats:italic>, methylated in tumors (MINT)1, MINT2, MINT31, and <jats:italic>CDKN2A<\/jats:italic>. The <jats:italic>BRAF<\/jats:italic> V600E mutation was determined by sequencing exon 15. In microsatellite stable tumors, homozygous carriers of the G39E polymorphism had an increased risk of CIMP+ colon cancer (odds ratio (OR) 2.2, 95% confidence interval (CI) 1.1, 4.2) and <jats:italic>BRAF<\/jats:italic> V600E mutation (OR 3.1, 95% CI 1.01, 9.7) in a case\u2013control comparison. This finding was not observed in unstable tumors; however, power may have been low to detect an association. Age at diagnosis, family history, and alcohol use did not interact with <jats:italic>MSH6<\/jats:italic> G39E and CIMP. The <jats:italic>MSH6<\/jats:italic> G39E germline polymorphism may be associated with CIMP+ colon cancer. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mc.20566","type":"journal-article","created":{"date-parts":[[2009,7,7]],"date-time":"2009-07-07T02:19:03Z","timestamp":1246933143000},"page":"989-994","source":"Crossref","is-referenced-by-count":11,"title":["<i>MSH6<\/i> G39E polymorphism and CpG island methylator phenotype in colon cancer"],"prefix":"10.1002","volume":"48","author":[{"given":"Karen","family":"Curtin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wade S.","family":"Samowitz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roger K.","family":"Wolff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bette 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