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Inhibition of proliferation of four cancer cell lines compared with normal human hepatic L\u2010O2 cells, cancer cell apoptosis, and expression of related mRNA and protein were detected using the methyl thiazolyl tetrazolium (MTT), flow cytometry, reverse transcription polymerase chain reaction (RT\u2010PCR), Western blot, and DNA ladder assays, and electron microscopy and immunocytochemistry. DBDCT decreased cancer cell proliferation rates in a dose\u2010 and time\u2010dependent manner and changed the cycle distribution of SGC\u20107901 cells; the proportion of cells in G<jats:sub>0<\/jats:sub>\u2013G<jats:sub>1<\/jats:sub> phase was increased, whereas the numbers in S and G<jats:sub>2<\/jats:sub>\u2013M phases were decreased. Blockade of the cell cycle was perhaps associated with increased levels of p21, p27, p53 and the decreased level of proliferating cell nuclear antigen (PCNA). Apoptosis was characterized by DNA fragmentation, chromosomal condensation, apoptotic bodies, sub\u2010G<jats:sub>1<\/jats:sub> peaks, and an increased apoptotic rate, as shown using the annexin V\u2010FITC method. Pretreatment of cells with <jats:italic>N<\/jats:italic>\u2010acetylcysteine and caspase\u20109 inhibitor could reduce growth inhibition and DBDCT\u2010induced apoptosis. The results showed that DBDCT\u2010mediated cell\u2010cycle arrest might occur through the induction of p21 in a p53\u2010dependent manner and that DBDCT induction of the mitochondrial apoptotic signaling pathway is perhaps mediated by increasing Bax\/Bcl\u20102 ratios, which result in the loss of \u0394\u03a8<jats:sub>m<\/jats:sub>, release of cytochrome <jats:italic>c<\/jats:italic> into the cytoplasm, activation of caspase\u20103 and \u20109, and increased reactive oxygen species (ROS) generation. Mol. Carcinog. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mc.20623","type":"journal-article","created":{"date-parts":[[2010,3,25]],"date-time":"2010-03-25T04:40:35Z","timestamp":1269492035000},"page":"566-581","source":"Crossref","is-referenced-by-count":32,"title":["Mechanisms by which the antitumor compound di\u2010<i>n<\/i>\u2010butyl\u2010di\u2010(4\u2010chlorobenzohydroxamato)tin(IV) induces apoptosis and the mitochondrial\u2010mediated signaling pathway in human cancer SGC\u20107901 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