{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T03:31:46Z","timestamp":1773113506921,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2010,8,18]],"date-time":"2010-08-18T00:00:00Z","timestamp":1282089600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Carcinogenesis"],"published-print":{"date-parts":[[2010,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The chromosomal 3q25.31 region was consistently amplified in primary cancer of cervix (CACX). <jats:italic>CyclinL1<\/jats:italic> is a candidate gene of this region and already have been implicated as an oncogene in head and neck cancers. In this study, we aimed to investigate the involvement of <jats:italic>CyclinL1<\/jats:italic> in cervical carcinogenesis and for this purpose its copy number variation (CNV) was studied in 23 cervical intraepithelial neoplasia (CIN) and 110 CACX samples. In CIN lesions <jats:italic>CyclinL1<\/jats:italic> was not amplified; however, the amplification frequency was 16% (9\/56) in stage I\/II tumors which remained comparable during subsequent stages of tumorigenesis. This implied association of <jats:italic>CyclinL1<\/jats:italic> amplification with development of early invasiveness. Quantitation of mRNA expression revealed 2.6\u2009\u00b1\u20091.53\u2010fold overexpression of this gene in primary CACX. The amplification\/copy number gain of <jats:italic>CyclinL1<\/jats:italic> and its mRNA profile were concordant, in tumors. Immunohistochemical (IHC) analysis in primary CACX, cell lines: SiHa and HeLa revealed intense nuclear expression of cyclinL1, which was further confirmed by Western blot in the cell lines. However 47% (7\/15) CACX samples expressed high\/intermediate level of cyclin L1. Kaplan\u2013Meier survival analysis indicated <jats:italic>CyclinL1<\/jats:italic> amplification as a determinant of poor patient outcome. Tumor radio\u2010resistance developed as a consequence of <jats:italic>CyclinL1<\/jats:italic> amplification. Cox multivariate analysis revealed that multiparous (\u22655) CACX patients with amplified <jats:italic>CyclinL1<\/jats:italic> locus along with advanced tumor stage (III\/IV) had worst prognosis. Our data suggest importance of <jats:italic>CyclinL1<\/jats:italic> in cervical carcinogenesis with its associated pathways viz: pre\u2010mRNA splicing, cell\u2010cycle regulation (G<jats:sub>0<\/jats:sub>\/G<jats:sub>1<\/jats:sub> and G<jats:sub>2<\/jats:sub>\/M) being potential targets of therapeutic interventions in CACX. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/mc.20671","type":"journal-article","created":{"date-parts":[[2010,8,19]],"date-time":"2010-08-19T03:05:32Z","timestamp":1282187132000},"page":"935-943","source":"Crossref","is-referenced-by-count":16,"title":["Amplification of <i>CyclinL1<\/i> in uterine cervical carcinoma has prognostic implications"],"prefix":"10.1002","volume":"49","author":[{"given":"Sraboni","family":"Mitra","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dipanjana","family":"Mazumder 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