{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T05:50:19Z","timestamp":1780638619593,"version":"3.54.1"},"reference-count":37,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2011,8,1]],"date-time":"2011-08-01T00:00:00Z","timestamp":1312156800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Carcinogenesis"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study aimed to understand the importance of <jats:italic>RASSF1A<\/jats:italic> and <jats:italic>CACNA2D2<\/jats:italic>, located in chromosomal 3p21.31 region, in the development of uterine cervical carcinoma (CACX). To this end, firstly the expression (RNA) profiles of <jats:italic>RASSF1A<\/jats:italic> and <jats:italic>CACNA2D2<\/jats:italic> were screened in primary cervical carcinoma (CACX) samples which indicated highly reduced expression for both genes. Thereafter alterations (deletion\/methylation) of these genes were analyzed in 23 cervical intraepithelial neoplasia (CIN) and 110 CACX samples. In CIN, deletion was observed only for <jats:italic>RASSF1A<\/jats:italic> (26%), whereas methylation was in the following order: <jats:italic>RASSF1A<\/jats:italic> (35%)\u2009&gt;\u2009<jats:italic>CACNA2D2<\/jats:italic> (9%). However, in CACX their deletion frequencies were the same (50%) and methylation frequencies were comparable <jats:italic>RASSF1A<\/jats:italic> (33%), <jats:italic>CACNA2D2<\/jats:italic> (27%). The reduced expression and molecular alterations of these genes were concordant. Overall alterations of <jats:italic>RASSF1A<\/jats:italic> showed association with CIN lesions and <jats:italic>CACNA2D2<\/jats:italic> with disease progression from CIN\u2009\u2192\u2009stage I\/II. Interestingly, alterations of these genes showed significant association in CACX suggesting possible functional synergism during tumor progression. Alterations of <jats:italic>RASSF1A<\/jats:italic> and <jats:italic>CACNA2D2<\/jats:italic> predicted poor prognosis for the patients. Moreover, <jats:italic>RASSF1A<\/jats:italic> alterations along with multiparity (\u22655\u2009yr) and early sexual debut (&lt;19\u2009yr) were determinants of worse prognosis. Our data suggests the association of <jats:italic>RASSF1A<\/jats:italic> and <jats:italic>CACNA2D2<\/jats:italic> in cervical carcinogenesis and its importance in early diagnosis and prognosis of the tumor. \u00a9 2011 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/mc.20837","type":"journal-article","created":{"date-parts":[[2011,8,1]],"date-time":"2011-08-01T12:07:20Z","timestamp":1312200440000},"page":"723-733","source":"Crossref","is-referenced-by-count":17,"title":["Alterations of <i>RASSF1A<\/i> in premalignant cervical lesions: Clinical and prognostic significance"],"prefix":"10.1002","volume":"51","author":[{"given":"Sraboni","family":"Mitra","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dipanjana","family":"Mazumder (Indra)","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Partha 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