{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T09:12:45Z","timestamp":1762161165771,"version":"3.41.0"},"reference-count":40,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2015,9,17]],"date-time":"2015-09-17T00:00:00Z","timestamp":1442448000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000646","name":"Japan Society for the Promotion of Science London","doi-asserted-by":"publisher","award":["24590493"],"award-info":[{"award-number":["24590493"]}],"id":[{"id":"10.13039\/501100000646","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Carcinogenesis"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:sec><jats:label\/><jats:p>Lysophosphatidic acid (LPA) signaling via LPA receptors (LPA<jats:sub>1<\/jats:sub>to LPA<jats:sub>6<\/jats:sub>) mediates a variety of cellular functions, including cell motility. In the present study, we investigated the effects of LPA receptors on cell motile activity during multi\u2010stage hepatocarcinogenesis in rat liver epithelial WB\u2010F344 cells treated with chemical liver carcinogens. Cells were treated with a initiator (N\u2010nitrosodiethylamine (DEN)) and three promoters (phenobarbital (PB), okadaic acid (OA) and clofibrate) every 24\u2009h for 2 days. Cell motile activity was elevated by DEN, correlating with<jats:italic>Lpar3<\/jats:italic>expression. PB, OA, and clofibrate elevated<jats:italic>Lpar1<\/jats:italic>expression and inhibited cell motile activity. To evaluate the effects of long\u2010term treatment on cell motility, cells were treated with DEN and\/or PB for at least 6 months.<jats:italic>Lpar3<\/jats:italic>expression and cell motile activity were significantly elevated by the long\u2010term DEN treatment with or without further PB treatment. In contrast, long\u2010term PB treatment with or without further DEN elevated<jats:italic>Lpar1<\/jats:italic>expression and inhibited cell motility. When the synthesis of extracellular LPA was blocked by a potent ATX inhibitor S32826 before cell motility assay, the cell motility induced by DEN and PB was markedly suppressed. These results suggest that activation of the different LPA receptors may regulate the biological functions of cells treated with chemical carcinogens. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mc.22410","type":"journal-article","created":{"date-parts":[[2015,9,17]],"date-time":"2015-09-17T06:01:29Z","timestamp":1442469689000},"page":"1573-1583","source":"Crossref","is-referenced-by-count":8,"title":["Different induction of LPA receptors by chemical liver carcinogens regulates cellular functions of liver epithelial WB\u2010F344 cells"],"prefix":"10.1002","volume":"55","author":[{"given":"Miku","family":"Hirane","sequence":"first","affiliation":[{"name":"Division of Molecular Oncology Department of Life Science Faculty of Science and Engineering, Kinki University Kowakae, Higashiosaka Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuhei","family":"Ishii","sequence":"additional","affiliation":[{"name":"Division of Molecular 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