{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T16:04:30Z","timestamp":1770566670007,"version":"3.49.0"},"reference-count":45,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2016,2,3]],"date-time":"2016-02-03T00:00:00Z","timestamp":1454457600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81471176"],"award-info":[{"award-number":["81471176"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81301045"],"award-info":[{"award-number":["81301045"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Carcinogenesis"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:sec><jats:label\/><jats:p>Methylation of the neurofibromatosis type 2 (NF2) gene in low\u2010grade meningioma (WHO grade I) has crucial roles in tumorigenesis and development. Meningioma formation might also occur in the setting of an inflammatory microenvironment. However, the association between inflammation and the methylation of NF2 remains unclear. The present study investigates the role and regulatory mechanism of IL\u20101\u03b2, one of the most important pro\u2010inflammatory cytokines, in the methylation of NF2 in benign meningioma. Three primary low\u2010grade meningioma cells and leptomeningeal cells were cultured. CCK\u20108 and BrdU assays demonstrated that proliferation of meningioma\/leptomeningeal cells treated with IL\u20101\u03b2 occurred in a dose\u2010 and time\u2010dependent manner. Methylation\u2010specific PCR verified that IL\u20101\u03b2 induced methylation of the NF2 promoter and decreased NF2\/merlin expression in meningioma\/leptomeningeal cells. Real\u2010time PCR, western blotting, and immunofluorescence showed that IL\u20101\u03b2 up\u2010regulated DNMT1 in meningioma cells and DNMT1\/3b in leptomeningeal cells but did not up\u2010regulate DNMT3a. After co\u2010treatment with the DNMT inhibitor 5\u2010Aza\u20102\u2032\u2010deoxycytidine and DNMT siRNA, methylation of NF2 induced by IL\u20101\u03b2 was attenuated and merlin expression was restored. Furthermore, we showed that DNMT1 in meningiomas and DNMT1\/3b in leptomeninges were regulated via activation of the MAPK (p38, ERK, JNK) and NF\u2010\u03baB pathways. These results suggest that IL\u20101\u03b2 induces methylation of NF2 by up\u2010regulating DNMT1 in benign meningioma cells and DNMT1\/3b in leptomeningeal cells via MAPK and NF\u2010\u03baB pathways. Therefore, NF2 methylation is a linker between IL\u20101\u03b2 and tumor development, and DNMTs might be potential therapeutic targets in meningioma for regulating NF2 and inhibiting tumor development. \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mc.22467","type":"journal-article","created":{"date-parts":[[2016,2,3]],"date-time":"2016-02-03T19:34:07Z","timestamp":1454528047000},"page":"2268-2277","source":"Crossref","is-referenced-by-count":21,"title":["The role and regulatory mechanism of IL\u20101\u03b2 on the methylation of the NF2 gene in benign meningiomas and leptomeninges"],"prefix":"10.1002","volume":"55","author":[{"given":"Baofeng","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Neurosurgery, Ruijin Hospital Shanghai Jiao Tong University School of Medicine Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenwen","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of 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