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Sixty\u2010four canine sarcoma samples (20 snap\u2010frozen, 44 FFPE) as well as six canine sarcoma cell lines were screened for ALT by C\u2010circle assay. ALT was further evaluated by measuring telomere length via qPCR and telomere restriction\u2010fragments including pulsed\u2010field electrophoresis. ALT\u2010associated proteins were validated by immunohistochemistry. Further, telomerase activity (TA) and gene expression were analyzed by TRAP and qPCR. DNA from 20 human neuroblastomas and 8 sarcoma cell lines served as comparative controls. ALT was detected in 9.4% (6\/64) canine sarcomas including aggressive subtypes as hemangiosarcoma, osteosarcoma, and histiocytic sarcoma. C\u2010circle levels were comparable with human ALT\u2010positive controls. All ALT tumors demonstrated loss of ATRX expression and 5\/6 showed strong p53 expression. TA was detected in 93% (14\/15) snap\u2010frozen samples including a sarcoma with ALT activity. This tumor showed long heterogeneous telomeres, and a high level of colocalization of DAXX with telomeres. One sarcoma was ALT and TA negative. All canine and human sarcoma cell lines were ALT negative. In this study, we demonstrated that canine sarcomas use ALT. As in humans, ALT was identified in aggressive sarcomas subtypes and coexisted with TA in one tumor. Overall, canine sarcomas seem to share many similarities with their human counterparts and appear an attractive model for comparative telomere research. \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/mc.22546","type":"journal-article","created":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T22:34:31Z","timestamp":1472769271000},"page":"923-935","source":"Crossref","is-referenced-by-count":11,"title":["Alternative lengthening of telomeres does exist in various canine sarcomas"],"prefix":"10.1002","volume":"56","author":[{"given":"Theresa","family":"Kreilmeier","sequence":"first","affiliation":[{"name":"Department for Companion Animals and Horses University of Veterinary Medicine Vienna Vienna Austria"},{"name":"Institute of Cancer Research Comprehensive Cancer Center Medical University of Vienna Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Sampl","sequence":"additional","affiliation":[{"name":"Institute 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