{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T02:26:33Z","timestamp":1789179993959,"version":"build-2803163510"},"reference-count":68,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2020,3,5]],"date-time":"2020-03-05T00:00:00Z","timestamp":1583366400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009634","name":"Susan G. Komen","doi-asserted-by":"publisher","award":["PDF17480342"],"award-info":[{"award-number":["PDF17480342"]}],"id":[{"id":"10.13039\/100009634","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007804","name":"Oregon Clinical and Translational Research Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007804","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Carcinogenesis"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>A hallmark of ductal carcinoma in situ (DCIS) progression is a loss of the surrounding ductal myoepithelium. However, whether compromise in myoepithelial differentiation, rather than overt cellular loss, can be used to predict the risk of DCIS progression is unknown. Here we address this question utilizing pure and mixed DCIS cases (N\u2009=\u200930) as surrogates for DCIS at low and high\u00a0risk for progression, respectively. We used multiplex immunohistochemical staining to evaluate the relationship between myoepithelial cell differentiation and lymphoid immune cell types associated with poor prognostic DCIS. Our results show that myoepithelial calponin\u20101 discriminates between pure and mixed DCIS lesions better than histological subtype, presence of necrosis, or nuclear grade. Additionally, focal loss of myoepithelial cells associated with increased PD\u20101+CD8+ T cells, which suggests a link between the myoepithelium and immune surveillance. To identify associations between calponin\u20101 expression and immune response, we performed unsupervised hierarchical clustering of myoepithelial and immune cell biomarkers on 219 DCIS lesions from 30 cases. Notably, the majority of pure (low\u2010risk) DCIS lesions clustered in a high calponin\u20101, T cell low group, whereas the majority of mixed (high\u2010risk) DCIS lesions clustered in a low calponin\u20101, T cell high group, specifically with CD8+ and PD\u20101+CD8+ T cells. However, a subset of pure DCIS lesions had a similar calponin\u20101 and immune signature as the majority of mixed DCIS lesions, which have low calponin\u20101 and T cell enrichment\u2014raising the possibility that these pure DCIS lesions might be at a high\u00a0risk for progression.<\/jats:p>","DOI":"10.1002\/mc.23171","type":"journal-article","created":{"date-parts":[[2020,3,5]],"date-time":"2020-03-05T06:37:26Z","timestamp":1583390246000},"page":"701-712","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Loss of myoepithelial calponin\u20101 characterizes high\u2010risk ductal carcinoma in situ cases, which are further stratified by T cell composition"],"prefix":"10.1002","volume":"59","author":[{"given":"Elizabeth","family":"Mitchell","sequence":"first","affiliation":[{"name":"Department of Cell, Developmental, and Cancer Biology Oregon Health and Science University  Portland 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Health Research Kaiser Permanente Northwest  Portland Oregon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4244-987X","authenticated-orcid":false,"given":"Pepper","family":"Schedin","sequence":"additional","affiliation":[{"name":"Department of Cell, Developmental, and Cancer Biology Oregon Health and Science University  Portland Oregon"},{"name":"Cancer Prevention and Control, Knight Cancer Institute Oregon Health and Science University  Portland Oregon"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,3,5]]},"reference":[{"key":"e_1_2_9_2_1","volume-title":"Breast Cancer Facts & Figures 2017\u20102018","author":"American Cancer 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