{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T20:22:10Z","timestamp":1760300530085,"version":"3.37.3"},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,12,31]],"date-time":"2020-12-31T00:00:00Z","timestamp":1609372800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Carcinogenesis"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>MicroRNA (miR)\u2212141\u20103p, which functions as an oncogene in multiple malignancies, has been shown to be highly overexpressed in esophageal cancer cells in our previous work. miR\u2010141\u20103p is predicted to bind the messenger RNA (mRNA) of tuberous sclerosis complex 1 (TSC1), a tumor suppressor, with high affinity. In this study, we investigated the expression and functional interaction between miR\u2010141\u20103p and TSC1 in esophageal cancer cells. Experiments were conducted in four esophageal cancer lines and in tumor cells isolated from human esophageal cancer specimens by laser capture microdissection. miR\u2010141\u20103p expression was measured by real time and droplet digital PCR. Biotinylated RNA pull\u2010down and luciferase reporter assays were used to assess binding. miR\u2010141\u20103p function was tested by assessing proliferation, migration, invasion, and induction of autophagy following its silencing. We found that miR\u2010141\u20103p levels were increased in TE7, OE33, and TE10 esophageal cancer cells compared to FLO\u20101 cells, with similar heterogeneity observed in human esophageal cancer specimens. Silencing of miR\u2010141\u20103p led to increased TSC1 protein expression in these cells and was associated with increased TSC1 translation. Binding studies reveal that miR\u2010141\u20103p binds to each of the predicted binding sites in the 3\u2032\u2010untranslated region of TSC1 mRNA. Following miR\u2010141\u20103p silencing, TE7, OE33, and TE10 cells exhibited decreased proliferation, migration, and invasion, as well as enhanced autophagy. Importantly, these phenotypic effects were replicated by overexpression of TSC1 alone in these cells. Our results indicate that miR\u2010141\u20103p functions in an oncogenic capacity in a subset of esophageal cancer cells, in part by suppressing TSC1 expression.<\/jats:p>","DOI":"10.1002\/mc.23274","type":"journal-article","created":{"date-parts":[[2020,12,31]],"date-time":"2020-12-31T18:22:24Z","timestamp":1609438944000},"page":"125-137","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["MicroRNA\u2010141\u20103p regulates cellular proliferation, migration, and invasion in esophageal cancer by targeting tuberous sclerosis complex 1"],"prefix":"10.1002","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3675-7051","authenticated-orcid":false,"given":"Pornima","family":"Phatak","sequence":"first","affiliation":[{"name":"Birmingham Veterans Affairs Medical Center  Birmingham Alabama USA"},{"name":"Department of Surgery University of Alabama at Birmingham  Birmingham Alabama USA"},{"name":"Baltimore Veterans Affairs Medical Center  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Noe","sequence":"additional","affiliation":[{"name":"Department of Pathology Johns Hopkins School of Medicine  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaushal","family":"Asrani","sequence":"additional","affiliation":[{"name":"Department of Pathology Johns Hopkins School of Medicine  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ingrid E.","family":"Chesnick","sequence":"additional","affiliation":[{"name":"Baltimore Veterans Affairs Medical Center  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruce D.","family":"Greenwald","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Maryland School of Medicine  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James M.","family":"Donahue","sequence":"additional","affiliation":[{"name":"Birmingham Veterans Affairs Medical Center  Birmingham Alabama USA"},{"name":"Department of Surgery University of Alabama at Birmingham  Birmingham Alabama USA"},{"name":"Baltimore Veterans Affairs Medical Center  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,12,31]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21492"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature20805"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41588-018-0331-5"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pathol-012513-104715"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbior.2014.09.013"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2015.271"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2016.12.045"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2017.5653"},{"key":"e_1_2_9_10_1","first-page":"2565","article-title":"Inhibition of miR\u2010141 reverses cisplatin resistance in non\u2010small cell lung cancer cells via upregulation of programmed cell death protein 4","volume":"20","author":"Fu WF","year":"2016","journal-title":"Eur Rev Med Pharmacol Sci"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2016.5214"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2016.01.025"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb999"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.202476899"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jso.23585"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-18-2049"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2016-312734"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1530\/JOE-17-0560"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00432-017-2550-z"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpg.2018.11.013"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-016-2816-9"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/jhg.2011.1"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.2174\/1574892814666181231142136"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2012.06.004"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2018.05.025"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2017.129"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-008-0307-5"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.093708"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/45257"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI20039"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.03.048"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1104361108"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2152"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2016.1271514"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1204592"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v23.i23.4243"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1177\/1010428317705748"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-016-2123-6"}],"container-title":["Molecular Carcinogenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mc.23274","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/mc.23274","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/mc.23274","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,3]],"date-time":"2023-09-03T10:43:56Z","timestamp":1693737836000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/mc.23274"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12,31]]},"references-count":37,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2021,2]]}},"alternative-id":["10.1002\/mc.23274"],"URL":"https:\/\/doi.org\/10.1002\/mc.23274","archive":["Portico"],"relation":{},"ISSN":["0899-1987","1098-2744"],"issn-type":[{"type":"print","value":"0899-1987"},{"type":"electronic","value":"1098-2744"}],"subject":[],"published":{"date-parts":[[2020,12,31]]},"assertion":[{"value":"2020-09-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-31","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}