{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T03:46:56Z","timestamp":1790826416951,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,12,23]],"date-time":"2022-12-23T00:00:00Z","timestamp":1671753600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Carcinogenesis"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Endometrial carcinoma is a common gynecological malignant tumor, small nucleolar RNAs (snoRNAs) are involved in cancer development. However, researches on the roles of snoRNAs in endometrial carcinoma are limited. The expression levels of snoRNAs in endometrial cancer tissues were analyzed using The Cancer Genome Atlas (TCGA) database. Antisense oligonucleotides (ASOs) and plasmids were used for transfection. Moreover, CCK\u20108, EdU, wound\u2010healing assay, transwell, cell apoptosis, western blotting, and xenograft model were employed to examine the biological functions of related molecules. real\u2010time reverse transcription polymerase chain reaction and western blotting were performed to detect messenger\u00a0RNA (mRNA) and protein levels. Including bioinformatics, fluorescence in situ hybridization, RNA pulldown, actinomycin D and RTL\u2010P assays were also carried out to explore the molecular mechanism. Analysis of data from TCGA showed that the expression level of small nucleolar RNA, C\/D box 60 (SNORD60) in endometrial cancer tissues is observably higher than that in normal endometrial tissues. Further research suggested that SNORD60 played a carcinogenic role both in vitro and in vivo, and significantly upregulated the expression of <jats:italic>PIK3CA<\/jats:italic>. However, the carcinogenic effects can be reversed by knocking down fibrillarin (<jats:italic>FBL<\/jats:italic>) or <jats:italic>PIK3CA<\/jats:italic>.\u00a0SNORD60 forms complexes by binding with 2\u2032\u2010O\u2010methyltransferase fibrillarin, thus catalyzes the 2\u2032\u2010O\u2010methylation (Nm) modification of <jats:italic>PIK3CA<\/jats:italic> mRNA and modulates the PI3K\/AKT\/mTOR signaling pathway, so as to promote the development of endometrial cancer. In short, SNORD60 might become a new biomarker for the therapy of endometrial cancer in the future and provide new strategies for diagnosis and treatment.<\/jats:p>","DOI":"10.1002\/mc.23495","type":"journal-article","created":{"date-parts":[[2022,12,23]],"date-time":"2022-12-23T11:51:30Z","timestamp":1671796290000},"page":"413-426","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["SNORD60 promotes the tumorigenesis and progression of endometrial cancer through binding <i>PIK3CA<\/i> and regulating PI3K\/AKT\/mTOR signaling pathway"],"prefix":"10.1002","volume":"62","author":[{"given":"Wu","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Department of Gynecologic Oncology Research Office, Guangdong Provincial Key Laboratory of Major Obstetric Diseases The Third Affiliated Hospital of Guangzhou Medical University Guangzhou 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